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Protective role of superoxide dismutases against ionizing radiation in yeast.
Biochim Biophys Acta. 2001 May 03; 1526(2):191-8.BB

Abstract

The protective role of superoxide dismutases (SODs) against ionizing radiation, which generates reactive oxygen species (ROS) harmful to cellular function, was investigated in the wild-type and in mutant yeast strains lacking cytosolic CuZnSOD (sod1Delta), mitochondrial MnSOD (sod2Delta), or both SODs (sod1Deltasod2Delta). Upon exposure to ionizing radiation, there was a distinct difference between these strains in regard to viability and the level of protein carbonyl content, which is the indicative marker of oxidative damage to protein, intracellular H2O2 level, as well as lipid peroxidation. When the oxidation of 2',7'-dichlorofluorescin was used to examine the hydroperoxide production in yeast cells, the SOD mutants showed a higher degree of increase in fluorescence upon exposure to ionizing radiation as compared to wild-type cells. These results indicated that mutants deleted for SOD genes were more sensitive to ionizing radiation than isogenic wild-type cells. Induction and inactivation of other antioxidant enzymes, such as catalase, glucose 6-phosphate dehydrogenase, and glutathione reductase, were observed after their exposure to ionizing radiation both in wild-type and in mutant cells. However, wild-type cells maintained significantly higher activities of antioxidant enzymes than did mutant cells. These results suggest that both CuZnSOD and MnSOD may play a central role in protecting cells against ionizing radiation through the removal of ROS, as well as in the protection of antioxidant enzymes.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, 702-701, Taegu, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11325541

Citation

Lee, J H., et al. "Protective Role of Superoxide Dismutases Against Ionizing Radiation in Yeast." Biochimica Et Biophysica Acta, vol. 1526, no. 2, 2001, pp. 191-8.
Lee JH, Choi IY, Kil IS, et al. Protective role of superoxide dismutases against ionizing radiation in yeast. Biochim Biophys Acta. 2001;1526(2):191-8.
Lee, J. H., Choi, I. Y., Kil, I. S., Kim, S. Y., Yang, E. S., & Park, J. W. (2001). Protective role of superoxide dismutases against ionizing radiation in yeast. Biochimica Et Biophysica Acta, 1526(2), 191-8.
Lee JH, et al. Protective Role of Superoxide Dismutases Against Ionizing Radiation in Yeast. Biochim Biophys Acta. 2001 May 3;1526(2):191-8. PubMed PMID: 11325541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective role of superoxide dismutases against ionizing radiation in yeast. AU - Lee,J H, AU - Choi,I Y, AU - Kil,I S, AU - Kim,S Y, AU - Yang,E S, AU - Park,J W, PY - 2001/4/28/pubmed PY - 2001/6/15/medline PY - 2001/4/28/entrez SP - 191 EP - 8 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1526 IS - 2 N2 - The protective role of superoxide dismutases (SODs) against ionizing radiation, which generates reactive oxygen species (ROS) harmful to cellular function, was investigated in the wild-type and in mutant yeast strains lacking cytosolic CuZnSOD (sod1Delta), mitochondrial MnSOD (sod2Delta), or both SODs (sod1Deltasod2Delta). Upon exposure to ionizing radiation, there was a distinct difference between these strains in regard to viability and the level of protein carbonyl content, which is the indicative marker of oxidative damage to protein, intracellular H2O2 level, as well as lipid peroxidation. When the oxidation of 2',7'-dichlorofluorescin was used to examine the hydroperoxide production in yeast cells, the SOD mutants showed a higher degree of increase in fluorescence upon exposure to ionizing radiation as compared to wild-type cells. These results indicated that mutants deleted for SOD genes were more sensitive to ionizing radiation than isogenic wild-type cells. Induction and inactivation of other antioxidant enzymes, such as catalase, glucose 6-phosphate dehydrogenase, and glutathione reductase, were observed after their exposure to ionizing radiation both in wild-type and in mutant cells. However, wild-type cells maintained significantly higher activities of antioxidant enzymes than did mutant cells. These results suggest that both CuZnSOD and MnSOD may play a central role in protecting cells against ionizing radiation through the removal of ROS, as well as in the protection of antioxidant enzymes. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/11325541/Protective_role_of_superoxide_dismutases_against_ionizing_radiation_in_yeast_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4165(01)00126-X DB - PRIME DP - Unbound Medicine ER -