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[Generation of Mx-cre transgenic mice].
Yi Chuan Xue Bao. 2001; 28(4):313-6.YC

Abstract

To generate the transgenic mice expressing cyclization recombination enzyme, the recombinant gene, in which the coding region of cre gene is derived by the promoter of mouse Mx gene, was microinjected into pronuclei of fertilized mouse eggs. Founders of transgenic mice harbouring the recombinant gene were screened by polymerase chain reaction (PCR) at genomic DNA level and confirmed by Southern blot. One line of Mx-cre transgenic mice was obtained. Then, the Mx-cre transgenic mouse line was cultured and propagated.

Authors+Show Affiliations

Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

11329872

Citation

Yao, Y C., et al. "[Generation of Mx-cre Transgenic Mice]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 28, no. 4, 2001, pp. 313-6.
Yao YC, Zi XY, Li JX, et al. [Generation of Mx-cre transgenic mice]. Yi Chuan Xue Bao. 2001;28(4):313-6.
Yao, Y. C., Zi, X. Y., Li, J. X., Xiong, J., Xie, Q. D., Wang, X. M., & Hu, Y. P. (2001). [Generation of Mx-cre transgenic mice]. Yi Chuan Xue Bao = Acta Genetica Sinica, 28(4), 313-6.
Yao YC, et al. [Generation of Mx-cre Transgenic Mice]. Yi Chuan Xue Bao. 2001;28(4):313-6. PubMed PMID: 11329872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Generation of Mx-cre transgenic mice]. AU - Yao,Y C, AU - Zi,X Y, AU - Li,J X, AU - Xiong,J, AU - Xie,Q D, AU - Wang,X M, AU - Hu,Y P, PY - 2001/5/2/pubmed PY - 2001/5/18/medline PY - 2001/5/2/entrez SP - 313 EP - 6 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 28 IS - 4 N2 - To generate the transgenic mice expressing cyclization recombination enzyme, the recombinant gene, in which the coding region of cre gene is derived by the promoter of mouse Mx gene, was microinjected into pronuclei of fertilized mouse eggs. Founders of transgenic mice harbouring the recombinant gene were screened by polymerase chain reaction (PCR) at genomic DNA level and confirmed by Southern blot. One line of Mx-cre transgenic mice was obtained. Then, the Mx-cre transgenic mouse line was cultured and propagated. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/11329872/[Generation_of_Mx_cre_transgenic_mice]_ DB - PRIME DP - Unbound Medicine ER -