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Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin.
Biol Trace Elem Res. 2001 Feb; 79(2):149-59.BT

Abstract

The aim of this work was to determine the protective effects of intraperitoneally administered vitamin E and selenium (as Na2SeO3, Se) on the lipid peroxidation as thiobarbituric acid reactive substances (TBARS) and vitamin E levels, glutathione peroxidase (GSH-Px), reduced glutathione (GSH) activities in the plasma, red blood cell (RBC), liver, and muscle,of rats with streptozotocin-induced diabetes. Fifty adult male Wistar rats were used and all rats were randomly divided into five groups. The first group was used as a control and the second group as a diabetic control. A placebo was given to first and second groups by injection. The third group was intraperitoneally administered with vitamin E (20 mg over 24 h), the fourth group with Se (0.3 mg over 24 h), and the fifth group with vitamin E and Se combination (COM) (20 mg vitamin E + 0.3 mg Se over 24 h). This administration was done for 25 days and the TBARS, vitamin E, GSH-Px, GSH levels in the plasma, RBC, liver, and muscle samples were determined. The vitamin E level in the plasma and liver was significantly (p < 0.05) higher in the control than in the diabetic control group. Also, the TBARS levels in the RBC, liver, and muscle were significantly (p < 0.05) lower in the control than in the diabetic control group. However, GSH-Px and GSH activities in RBC, liver, and muscle were not statistically different between the control and the diabetic control groups. The vitamin E levels in plasma and liver (p < 0.01 and p < 0.001) and GSH-Px activities (p < 0.01, p < 0.001) in RBC were significantly higher in vitamin E, Se, and COM groups than in both control and diabetic control groups. However, the TBARS levels of RBC, muscle, and liver in vitamin E and Se administered groups were significantly (p < 0.05-p < 0.001, respectively) decreased. These results indicate that intraperitoneally administered vitamin E and Se have significant protective effects on the blood, liver, and muscle against oxidative damage of diabetes.

Authors+Show Affiliations

Naziroğlu M
Department of Physiology, Veterinary Faculty of Firat University, Elaziğ, Turkey.
Cay M
No affiliation info available

MeSH

AnimalsAntioxidantsDiabetes Mellitus, ExperimentalDose-Response Relationship, DrugGlutathioneGlutathione PeroxidaseInjections, IntraperitonealLipid PeroxidationLiverMaleMusclesOxygenRatsRats, WistarSeleniumThiobarbituric Acid Reactive SubstancesTime FactorsVitamin E

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11330521

Citation

Naziroğlu, M, and M Cay. "Protective Role of Intraperitoneally Administered Vitamin E and Selenium On the Antioxidative Defense Mechanisms in Rats With Diabetes Induced By Streptozotocin." Biological Trace Element Research, vol. 79, no. 2, 2001, pp. 149-59.
Naziroğlu M, Cay M. Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. Biol Trace Elem Res. 2001;79(2):149-59.
Naziroğlu, M., & Cay, M. (2001). Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. Biological Trace Element Research, 79(2), 149-59.
Naziroğlu M, Cay M. Protective Role of Intraperitoneally Administered Vitamin E and Selenium On the Antioxidative Defense Mechanisms in Rats With Diabetes Induced By Streptozotocin. Biol Trace Elem Res. 2001;79(2):149-59. PubMed PMID: 11330521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. AU - Naziroğlu,M, AU - Cay,M, PY - 2001/5/2/pubmed PY - 2001/10/26/medline PY - 2001/5/2/entrez SP - 149 EP - 59 JF - Biological trace element research JO - Biol Trace Elem Res VL - 79 IS - 2 N2 - The aim of this work was to determine the protective effects of intraperitoneally administered vitamin E and selenium (as Na2SeO3, Se) on the lipid peroxidation as thiobarbituric acid reactive substances (TBARS) and vitamin E levels, glutathione peroxidase (GSH-Px), reduced glutathione (GSH) activities in the plasma, red blood cell (RBC), liver, and muscle,of rats with streptozotocin-induced diabetes. Fifty adult male Wistar rats were used and all rats were randomly divided into five groups. The first group was used as a control and the second group as a diabetic control. A placebo was given to first and second groups by injection. The third group was intraperitoneally administered with vitamin E (20 mg over 24 h), the fourth group with Se (0.3 mg over 24 h), and the fifth group with vitamin E and Se combination (COM) (20 mg vitamin E + 0.3 mg Se over 24 h). This administration was done for 25 days and the TBARS, vitamin E, GSH-Px, GSH levels in the plasma, RBC, liver, and muscle samples were determined. The vitamin E level in the plasma and liver was significantly (p < 0.05) higher in the control than in the diabetic control group. Also, the TBARS levels in the RBC, liver, and muscle were significantly (p < 0.05) lower in the control than in the diabetic control group. However, GSH-Px and GSH activities in RBC, liver, and muscle were not statistically different between the control and the diabetic control groups. The vitamin E levels in plasma and liver (p < 0.01 and p < 0.001) and GSH-Px activities (p < 0.01, p < 0.001) in RBC were significantly higher in vitamin E, Se, and COM groups than in both control and diabetic control groups. However, the TBARS levels of RBC, muscle, and liver in vitamin E and Se administered groups were significantly (p < 0.05-p < 0.001, respectively) decreased. These results indicate that intraperitoneally administered vitamin E and Se have significant protective effects on the blood, liver, and muscle against oxidative damage of diabetes. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/11330521/Protective_role_of_intraperitoneally_administered_vitamin_E_and_selenium_on_the_antioxidative_defense_mechanisms_in_rats_with_diabetes_induced_by_streptozotocin_ DB - PRIME DP - Unbound Medicine ER -