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Effects of two highly monounsaturated oils on lipid composition and enzyme activities in rat jejunum.
Biosci Rep. 2000 Oct; 20(5):355-68.BR

Abstract

The effects of two monounsaturated fatty acid (MUFA) oils, olive oil (OO) and high-oleic sunflower oil (HOSO), with high content in oleic acid but differing in their non-fatty acid fraction, on brush-border membrane (BBM) lipid composition and fluidity and on mucosal enzyme activities of rat jejunum were studied. Animals were given semipurified diet with linoleic acid to prevent essential fatty acid deficiency (control group) or semipurified diet containing 10% of either OO or HOSO for 12 weeks. There was a significant decrease in the content of jejunal BBM phospholipids together with an increase in the level of free cholesterol in both oil-fed rats, when compared to control group. Although the increase in the BBM free cholesterol level was not statistically significant in HOSO-fed rats, a significant decrease in the phospholipid/free cholesterol ratio was found in both OO and HOSO-fed animals compared to control group. Rat jejunal BBM had a high level of free fatty acids which was increased in BBM isolated from OO and HOSO-fed animals. There was no statistical significant difference in the phospholipid distribution between the control and the OO group. However, HOSO-fed animals showed the lowest level of phosphatidylethanolamine together with the highest phosphatidylcholine content and the phosphatidylcholine/sphingomyelin ratio. The fatty acid pattern of jejunal BBM lipids was modified according to the major fatty acids in the oils. There was a decrease in both stearic acid (18:0) and linoleic acid (18:2 n-6), together with an increase in oleic acid (18:1 n-9) in jenunal BBM isolated from both oil experimental groups. All these results were accompanied by a significant increase in the BBM fluidity (as assessed by steady-state fluorescence polarization of diphenylhexatriene) isolated from oil-fed rat, when compared to control group. OO and HOSO-fed animals had the lowest activities of sucrase and maltase, while alkaline phosphatase activity only was decreased in HOSO-fed animals. The specific activity of maltase was not modified in any experimental rats. In summary, both MUFA oils induced similar effects on jejunal BBM lipid composition, fluidity, sucrase, maltase and lactase activities. Furthermore, HOSO intake resulted in a lowest alkaline phosphatase activity which was accompanied by changes in individual phospholipid composition. All these results suggest that effects of MUFA oils on jejunal BBM lipid composition and hydrolase activities are most likely due to the presence of high content of oleic acid rather than other components contained in the non-fatty acid of olive oil.

Authors+Show Affiliations

Deparamento Fisiología y Biología Animal, Facultad de Farmacia, Sevilla, Spain. vazquez@fafar.us.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11332598

Citation

Vázquez, C M., et al. "Effects of Two Highly Monounsaturated Oils On Lipid Composition and Enzyme Activities in Rat Jejunum." Bioscience Reports, vol. 20, no. 5, 2000, pp. 355-68.
Vázquez CM, Zanetti R, Santa-María C, et al. Effects of two highly monounsaturated oils on lipid composition and enzyme activities in rat jejunum. Biosci Rep. 2000;20(5):355-68.
Vázquez, C. M., Zanetti, R., Santa-María, C., & Ruíz-Gutiérrez, V. (2000). Effects of two highly monounsaturated oils on lipid composition and enzyme activities in rat jejunum. Bioscience Reports, 20(5), 355-68.
Vázquez CM, et al. Effects of Two Highly Monounsaturated Oils On Lipid Composition and Enzyme Activities in Rat Jejunum. Biosci Rep. 2000;20(5):355-68. PubMed PMID: 11332598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of two highly monounsaturated oils on lipid composition and enzyme activities in rat jejunum. AU - Vázquez,C M, AU - Zanetti,R, AU - Santa-María,C, AU - Ruíz-Gutiérrez,V, PY - 2001/5/3/pubmed PY - 2001/9/21/medline PY - 2001/5/3/entrez SP - 355 EP - 68 JF - Bioscience reports JO - Biosci Rep VL - 20 IS - 5 N2 - The effects of two monounsaturated fatty acid (MUFA) oils, olive oil (OO) and high-oleic sunflower oil (HOSO), with high content in oleic acid but differing in their non-fatty acid fraction, on brush-border membrane (BBM) lipid composition and fluidity and on mucosal enzyme activities of rat jejunum were studied. Animals were given semipurified diet with linoleic acid to prevent essential fatty acid deficiency (control group) or semipurified diet containing 10% of either OO or HOSO for 12 weeks. There was a significant decrease in the content of jejunal BBM phospholipids together with an increase in the level of free cholesterol in both oil-fed rats, when compared to control group. Although the increase in the BBM free cholesterol level was not statistically significant in HOSO-fed rats, a significant decrease in the phospholipid/free cholesterol ratio was found in both OO and HOSO-fed animals compared to control group. Rat jejunal BBM had a high level of free fatty acids which was increased in BBM isolated from OO and HOSO-fed animals. There was no statistical significant difference in the phospholipid distribution between the control and the OO group. However, HOSO-fed animals showed the lowest level of phosphatidylethanolamine together with the highest phosphatidylcholine content and the phosphatidylcholine/sphingomyelin ratio. The fatty acid pattern of jejunal BBM lipids was modified according to the major fatty acids in the oils. There was a decrease in both stearic acid (18:0) and linoleic acid (18:2 n-6), together with an increase in oleic acid (18:1 n-9) in jenunal BBM isolated from both oil experimental groups. All these results were accompanied by a significant increase in the BBM fluidity (as assessed by steady-state fluorescence polarization of diphenylhexatriene) isolated from oil-fed rat, when compared to control group. OO and HOSO-fed animals had the lowest activities of sucrase and maltase, while alkaline phosphatase activity only was decreased in HOSO-fed animals. The specific activity of maltase was not modified in any experimental rats. In summary, both MUFA oils induced similar effects on jejunal BBM lipid composition, fluidity, sucrase, maltase and lactase activities. Furthermore, HOSO intake resulted in a lowest alkaline phosphatase activity which was accompanied by changes in individual phospholipid composition. All these results suggest that effects of MUFA oils on jejunal BBM lipid composition and hydrolase activities are most likely due to the presence of high content of oleic acid rather than other components contained in the non-fatty acid of olive oil. SN - 0144-8463 UR - https://www.unboundmedicine.com/medline/citation/11332598/Effects_of_two_highly_monounsaturated_oils_on_lipid_composition_and_enzyme_activities_in_rat_jejunum_ L2 - https://portlandpress.com/bioscirep/article-lookup/doi/10.1023/a:1010377900745 DB - PRIME DP - Unbound Medicine ER -