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Increased osteoblast apoptosis in apert craniosynostosis: role of protein kinase C and interleukin-1.
Am J Pathol. 2001 May; 158(5):1833-42.AJ

Abstract

Apert syndrome is an autosomal dominant disorder characterized by premature cranial ossification resulting from fibroblast growth factor receptor-2 (FGFR-2)-activating mutations. We have studied the effects of the prominent S252W FGFR-2 Apert mutation on apoptosis and the underlying mechanisms in human mutant osteoblasts. In vivo analysis of terminal deoxynucleotidyl transferase-mediated nick-end labeling revealed premature apoptosis of mature osteoblasts and osteocytes in the Apert suture compared to normal coronal suture. In vitro, mutant osteoblasts showed increased apoptosis, as demonstrated by terminal deoxynucleotidyl transferase-mediated nick-end labeling analysis, trypan blue staining, and DNA fragmentation. Mutant osteoblasts also showed increased activity of caspase-8 and effector caspases (-3, -6, -7) constitutively. This was related to protein kinase C activation because the selective protein kinase C inhibitor calphostin C inhibited caspase-8, effector caspases, and apoptosis in mutant osteoblasts. Apert osteoblasts also showed increased expression of interleukin (IL)-1alpha, IL-1beta, Fas, and Bax, and decreased Bcl-2 levels. Specific neutralizing anti-IL-1 antibody reduced Fas levels, Bax expression, effector caspases activity, and apoptosis in mutant cells. Thus, the Apert S252W FGFR-2 mutation promotes apoptosis in human osteoblasts through activation of protein kinase C, overexpression of IL-1 and Fas, activation of caspase-8, and increased Bax/Bcl-2 levels, leading to increased effector caspases and DNA fragmentation. This identifies a complex FGFR-2 signaling pathway involved in the premature apoptosis induced by the Apert S252W FGFR-2 mutation in human calvaria osteoblasts.

Authors+Show Affiliations

INSERM U 349 Affiliated CNRS, Lariboisière Hospital, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11337381

Citation

Lemonnier, J, et al. "Increased Osteoblast Apoptosis in Apert Craniosynostosis: Role of Protein Kinase C and Interleukin-1." The American Journal of Pathology, vol. 158, no. 5, 2001, pp. 1833-42.
Lemonnier J, Haÿ E, Delannoy P, et al. Increased osteoblast apoptosis in apert craniosynostosis: role of protein kinase C and interleukin-1. Am J Pathol. 2001;158(5):1833-42.
Lemonnier, J., Haÿ, E., Delannoy, P., Fromigué, O., Lomri, A., Modrowski, D., & Marie, P. J. (2001). Increased osteoblast apoptosis in apert craniosynostosis: role of protein kinase C and interleukin-1. The American Journal of Pathology, 158(5), 1833-42.
Lemonnier J, et al. Increased Osteoblast Apoptosis in Apert Craniosynostosis: Role of Protein Kinase C and Interleukin-1. Am J Pathol. 2001;158(5):1833-42. PubMed PMID: 11337381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased osteoblast apoptosis in apert craniosynostosis: role of protein kinase C and interleukin-1. AU - Lemonnier,J, AU - Haÿ,E, AU - Delannoy,P, AU - Fromigué,O, AU - Lomri,A, AU - Modrowski,D, AU - Marie,P J, PY - 2001/5/5/pubmed PY - 2001/6/22/medline PY - 2001/5/5/entrez SP - 1833 EP - 42 JF - The American journal of pathology JO - Am J Pathol VL - 158 IS - 5 N2 - Apert syndrome is an autosomal dominant disorder characterized by premature cranial ossification resulting from fibroblast growth factor receptor-2 (FGFR-2)-activating mutations. We have studied the effects of the prominent S252W FGFR-2 Apert mutation on apoptosis and the underlying mechanisms in human mutant osteoblasts. In vivo analysis of terminal deoxynucleotidyl transferase-mediated nick-end labeling revealed premature apoptosis of mature osteoblasts and osteocytes in the Apert suture compared to normal coronal suture. In vitro, mutant osteoblasts showed increased apoptosis, as demonstrated by terminal deoxynucleotidyl transferase-mediated nick-end labeling analysis, trypan blue staining, and DNA fragmentation. Mutant osteoblasts also showed increased activity of caspase-8 and effector caspases (-3, -6, -7) constitutively. This was related to protein kinase C activation because the selective protein kinase C inhibitor calphostin C inhibited caspase-8, effector caspases, and apoptosis in mutant osteoblasts. Apert osteoblasts also showed increased expression of interleukin (IL)-1alpha, IL-1beta, Fas, and Bax, and decreased Bcl-2 levels. Specific neutralizing anti-IL-1 antibody reduced Fas levels, Bax expression, effector caspases activity, and apoptosis in mutant cells. Thus, the Apert S252W FGFR-2 mutation promotes apoptosis in human osteoblasts through activation of protein kinase C, overexpression of IL-1 and Fas, activation of caspase-8, and increased Bax/Bcl-2 levels, leading to increased effector caspases and DNA fragmentation. This identifies a complex FGFR-2 signaling pathway involved in the premature apoptosis induced by the Apert S252W FGFR-2 mutation in human calvaria osteoblasts. SN - 0002-9440 UR - https://www.unboundmedicine.com/medline/citation/11337381/Increased_osteoblast_apoptosis_in_apert_craniosynostosis:_role_of_protein_kinase_C_and_interleukin_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9440(10)64139-9 DB - PRIME DP - Unbound Medicine ER -