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[Effect of various trophic factors on bacterial translocation in experimental short bowel syndrome].
Cir Pediatr. 2001 Jan; 14(1):4-8.CP

Abstract

Massive bowel resection triggers an adaptive process in the remaining intestine in spite of which, bacterial translocation (BT) is frequent under these conditions. Several trophic factors, including growth hormone (GH), epidermal growth factor (EGF) and insuline (INS) are involved in the process of adaptation in short bowel syndrome (SBS). However, the effect of GH, EGF or INS on BT has not been investigated experimentally. The aim of the study was to test the hypothesis that GH, EGF or INS administration prevents BT in rats with SBS receiving only parenteral nutrition (PN). Thirty-seven adult Wistar rats underwent central venous cannulation and were randomly assigned to one of two groups receiving for ten days four treatment regimes: PN group (N = 10) fasting, all-in-one PN solution (300 mL/kg/24 h, 280 kcal/kg/24 h), 80% gut resection including ileo-cecal valve. GH group (N = 9) fasting, same PN regime and resection plus GH (1 mg/kg/d, s.c.). EGF group (N = 9): same PN regime and resection plus EGF (150 microgr/24 h, e.v.) INS group(N = 9): same PN regime and resection plus INS (1 U.I./100 g/24 h s.c.) At the end of the experiment the rats were exanguinated and mesenteric lymph nodes and samples of systemic and portal blood were obtained and cultured. Several samples of full-thickness jejunal wall were taken for measuring cell proliferation index (PCNA) and mucosal thickness. Jejunal mucosal thickness increased by 30%, 28% and 29% and PCNA index by 21%, 20% and 25% in GH, EGF and INS, treated rats respectively in comparison with those treated with PN alone. However, contrary to our expectations, BT expressed by positive culture of intestinal germs in systemic blood was demonstrated respectively in 44%, 40% and 28% of GH, EGF and INS animals, respectively, and in 0% of PN-only rats. Although exogenous GH, EGF or INS improves gut mucosal structure in rats with SBS treated with PN, it seems to increase rather than decrease mucosal permeability to intestinal germs in them.

Authors+Show Affiliations

Servicio de Cirugía Pediátrica, Hospital Aranzazu, Complejo Hospitalario Donostia, Osakidetza, San Sebastian. ieizagui@chdo.osakidetza.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

11339120

Citation

Eizaguirre, I, et al. "[Effect of Various Trophic Factors On Bacterial Translocation in Experimental Short Bowel Syndrome]." Cirugia Pediatrica : Organo Oficial De La Sociedad Espanola De Cirugia Pediatrica, vol. 14, no. 1, 2001, pp. 4-8.
Eizaguirre I, Aldazabal P, García N, et al. [Effect of various trophic factors on bacterial translocation in experimental short bowel syndrome]. Cir Pediatr. 2001;14(1):4-8.
Eizaguirre, I., Aldazabal, P., García, N., Orgiles, I., García-Arenzana, J. M., Ariz, C., Tovar, J. A., & Candelas, S. (2001). [Effect of various trophic factors on bacterial translocation in experimental short bowel syndrome]. Cirugia Pediatrica : Organo Oficial De La Sociedad Espanola De Cirugia Pediatrica, 14(1), 4-8.
Eizaguirre I, et al. [Effect of Various Trophic Factors On Bacterial Translocation in Experimental Short Bowel Syndrome]. Cir Pediatr. 2001;14(1):4-8. PubMed PMID: 11339120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of various trophic factors on bacterial translocation in experimental short bowel syndrome]. AU - Eizaguirre,I, AU - Aldazabal,P, AU - García,N, AU - Orgiles,I, AU - García-Arenzana,J M, AU - Ariz,C, AU - Tovar,J A, AU - Candelas,S, PY - 2001/5/8/pubmed PY - 2001/8/31/medline PY - 2001/5/8/entrez SP - 4 EP - 8 JF - Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica JO - Cir Pediatr VL - 14 IS - 1 N2 - Massive bowel resection triggers an adaptive process in the remaining intestine in spite of which, bacterial translocation (BT) is frequent under these conditions. Several trophic factors, including growth hormone (GH), epidermal growth factor (EGF) and insuline (INS) are involved in the process of adaptation in short bowel syndrome (SBS). However, the effect of GH, EGF or INS on BT has not been investigated experimentally. The aim of the study was to test the hypothesis that GH, EGF or INS administration prevents BT in rats with SBS receiving only parenteral nutrition (PN). Thirty-seven adult Wistar rats underwent central venous cannulation and were randomly assigned to one of two groups receiving for ten days four treatment regimes: PN group (N = 10) fasting, all-in-one PN solution (300 mL/kg/24 h, 280 kcal/kg/24 h), 80% gut resection including ileo-cecal valve. GH group (N = 9) fasting, same PN regime and resection plus GH (1 mg/kg/d, s.c.). EGF group (N = 9): same PN regime and resection plus EGF (150 microgr/24 h, e.v.) INS group(N = 9): same PN regime and resection plus INS (1 U.I./100 g/24 h s.c.) At the end of the experiment the rats were exanguinated and mesenteric lymph nodes and samples of systemic and portal blood were obtained and cultured. Several samples of full-thickness jejunal wall were taken for measuring cell proliferation index (PCNA) and mucosal thickness. Jejunal mucosal thickness increased by 30%, 28% and 29% and PCNA index by 21%, 20% and 25% in GH, EGF and INS, treated rats respectively in comparison with those treated with PN alone. However, contrary to our expectations, BT expressed by positive culture of intestinal germs in systemic blood was demonstrated respectively in 44%, 40% and 28% of GH, EGF and INS animals, respectively, and in 0% of PN-only rats. Although exogenous GH, EGF or INS improves gut mucosal structure in rats with SBS treated with PN, it seems to increase rather than decrease mucosal permeability to intestinal germs in them. SN - 0214-1221 UR - https://www.unboundmedicine.com/medline/citation/11339120/[Effect_of_various_trophic_factors_on_bacterial_translocation_in_experimental_short_bowel_syndrome]_ DB - PRIME DP - Unbound Medicine ER -