Tags

Type your tag names separated by a space and hit enter

Validated gas chromatographic-mass spectrometric assay for determination of the antifreezes ethylene glycol and diethylene glycol in human plasma after microwave-assisted pivalylation.
J Chromatogr B Biomed Sci Appl. 2001 Apr 25; 754(2):401-9.JC

Abstract

A gas chromatographic-mass spectrometric assay is described for identification and quantification of the antifreezes ethylene glycol (EG) and diethylene glycol (DEG) in plasma for early diagnosis of a glycol intoxication. After addition of 1,3-propanediol as internal standard, the plasma sample was deproteinized by acetone and an aliquot of the supernatant was evaporated followed by microwave-assisted pivalylation. After gas chromatographic separation, the glycols were first identified by comparison of the full mass spectra with reference spectra and then quantified. The quantification has been validated according to the criteria established by the Journal of Chromatography B. The assay was found to be selective. The calibration curves for EG and DEG were linear from 0.1 g/l to 1.0 g/l. The limit of detection for EG and DEG was 0.01 g/l and the limit of quantification for both was 0.1 g/l. The absolute recoveries were 50 and 65% for the low quality control samples and 51 and 73% for the high quality control samples of EG and DEG, respectively. Intra- and inter-day accuracy and precision were inside the required limits. The glycols in frozen plasma samples were stable for more than 6 months. The method was successfully applied to several authentic plasma samples from patients intoxicated with glycols. It has also been suitable for analysis of EG and DEG in urine.

Authors+Show Affiliations

Department of Experimental and Clinical Toxicology, Institute of Pharmacology and Toxicology, University of Saarland, Homburg, Saar, Germany. hans.maurer@med-rz.uni-saarland.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11339283

Citation

Maurer, H H., et al. "Validated Gas Chromatographic-mass Spectrometric Assay for Determination of the Antifreezes Ethylene Glycol and Diethylene Glycol in Human Plasma After Microwave-assisted Pivalylation." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 754, no. 2, 2001, pp. 401-9.
Maurer HH, Peters FT, Paul LD, et al. Validated gas chromatographic-mass spectrometric assay for determination of the antifreezes ethylene glycol and diethylene glycol in human plasma after microwave-assisted pivalylation. J Chromatogr B Biomed Sci Appl. 2001;754(2):401-9.
Maurer, H. H., Peters, F. T., Paul, L. D., & Kraemer, T. (2001). Validated gas chromatographic-mass spectrometric assay for determination of the antifreezes ethylene glycol and diethylene glycol in human plasma after microwave-assisted pivalylation. Journal of Chromatography. B, Biomedical Sciences and Applications, 754(2), 401-9.
Maurer HH, et al. Validated Gas Chromatographic-mass Spectrometric Assay for Determination of the Antifreezes Ethylene Glycol and Diethylene Glycol in Human Plasma After Microwave-assisted Pivalylation. J Chromatogr B Biomed Sci Appl. 2001 Apr 25;754(2):401-9. PubMed PMID: 11339283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated gas chromatographic-mass spectrometric assay for determination of the antifreezes ethylene glycol and diethylene glycol in human plasma after microwave-assisted pivalylation. AU - Maurer,H H, AU - Peters,F T, AU - Paul,L D, AU - Kraemer,T, PY - 2001/5/8/pubmed PY - 2001/8/24/medline PY - 2001/5/8/entrez SP - 401 EP - 9 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 754 IS - 2 N2 - A gas chromatographic-mass spectrometric assay is described for identification and quantification of the antifreezes ethylene glycol (EG) and diethylene glycol (DEG) in plasma for early diagnosis of a glycol intoxication. After addition of 1,3-propanediol as internal standard, the plasma sample was deproteinized by acetone and an aliquot of the supernatant was evaporated followed by microwave-assisted pivalylation. After gas chromatographic separation, the glycols were first identified by comparison of the full mass spectra with reference spectra and then quantified. The quantification has been validated according to the criteria established by the Journal of Chromatography B. The assay was found to be selective. The calibration curves for EG and DEG were linear from 0.1 g/l to 1.0 g/l. The limit of detection for EG and DEG was 0.01 g/l and the limit of quantification for both was 0.1 g/l. The absolute recoveries were 50 and 65% for the low quality control samples and 51 and 73% for the high quality control samples of EG and DEG, respectively. Intra- and inter-day accuracy and precision were inside the required limits. The glycols in frozen plasma samples were stable for more than 6 months. The method was successfully applied to several authentic plasma samples from patients intoxicated with glycols. It has also been suitable for analysis of EG and DEG in urine. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/11339283/Validated_gas_chromatographic_mass_spectrometric_assay_for_determination_of_the_antifreezes_ethylene_glycol_and_diethylene_glycol_in_human_plasma_after_microwave_assisted_pivalylation_ DB - PRIME DP - Unbound Medicine ER -