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Expression of cyclooxygenase-2 in patients with bladder carcinoma.
J Urol. 2001 May; 165(5):1468-72.JU

Abstract

PURPOSE

Cyclooxygenase-2 is considered to have an important role in the development of metastasis in cancer due to angiogenesis function. The expression of cyclooxygenase-2 was found to be up-regulated in colorectal carcinoma and other cancers. We investigated cyclooxygenase-1 and 2 expressions in patients with bladder cancer, chronic cystitis and normal bladder.

MATERIALS AND METHODS

A total of 118 specimens were obtained from patients treated at Osaka City University Hospital for bladder cancer, including 10 with chronic cystitis and 8 with normal bladder tissue. Immunohistochemistry, with affinity purified antibodies against human cyclooxygenase-1 and 2 that did not have cross-reactivity with each other, and reverse transcriptase polymerase chain reaction to study the messenger RNA expression were performed.

RESULTS

Although no marked expression of cyclooxygenase-2 was observed in the normal bladder, it was slightly seen in infiltrative inflammatory cells of chronic cystitis, and a higher expression was found in cancer cells. The extent and intensity of immunoreactive cyclooxygenase-2 polypeptides in cancer cells was statistically much greater than those in cells from normal bladder tissue. Moreover, correlation between cyclooxygenase-2 expression and tissue type or progression of bladder cancer was observed. Cyclooxygenase-2 expression was higher in grade 3 bladder cancer than in grade 1, and was higher in advanced than in early stage cancer.

CONCLUSIONS

These results demonstrate that generated cyclooxygenase-2 in the cells of patients with bladder cancer might be significant in the proliferation of bladder malignant cells and development of invasions.

Authors+Show Affiliations

Department of Urology, Osaka City University Medical School, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11342898

Citation

Yoshimura, R, et al. "Expression of Cyclooxygenase-2 in Patients With Bladder Carcinoma." The Journal of Urology, vol. 165, no. 5, 2001, pp. 1468-72.
Yoshimura R, Sano H, Mitsuhashi M, et al. Expression of cyclooxygenase-2 in patients with bladder carcinoma. J Urol. 2001;165(5):1468-72.
Yoshimura, R., Sano, H., Mitsuhashi, M., Kohno, M., Chargui, J., & Wada, S. (2001). Expression of cyclooxygenase-2 in patients with bladder carcinoma. The Journal of Urology, 165(5), 1468-72.
Yoshimura R, et al. Expression of Cyclooxygenase-2 in Patients With Bladder Carcinoma. J Urol. 2001;165(5):1468-72. PubMed PMID: 11342898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of cyclooxygenase-2 in patients with bladder carcinoma. AU - Yoshimura,R, AU - Sano,H, AU - Mitsuhashi,M, AU - Kohno,M, AU - Chargui,J, AU - Wada,S, PY - 2001/5/9/pubmed PY - 2001/5/26/medline PY - 2001/5/9/entrez SP - 1468 EP - 72 JF - The Journal of urology JO - J. Urol. VL - 165 IS - 5 N2 - PURPOSE: Cyclooxygenase-2 is considered to have an important role in the development of metastasis in cancer due to angiogenesis function. The expression of cyclooxygenase-2 was found to be up-regulated in colorectal carcinoma and other cancers. We investigated cyclooxygenase-1 and 2 expressions in patients with bladder cancer, chronic cystitis and normal bladder. MATERIALS AND METHODS: A total of 118 specimens were obtained from patients treated at Osaka City University Hospital for bladder cancer, including 10 with chronic cystitis and 8 with normal bladder tissue. Immunohistochemistry, with affinity purified antibodies against human cyclooxygenase-1 and 2 that did not have cross-reactivity with each other, and reverse transcriptase polymerase chain reaction to study the messenger RNA expression were performed. RESULTS: Although no marked expression of cyclooxygenase-2 was observed in the normal bladder, it was slightly seen in infiltrative inflammatory cells of chronic cystitis, and a higher expression was found in cancer cells. The extent and intensity of immunoreactive cyclooxygenase-2 polypeptides in cancer cells was statistically much greater than those in cells from normal bladder tissue. Moreover, correlation between cyclooxygenase-2 expression and tissue type or progression of bladder cancer was observed. Cyclooxygenase-2 expression was higher in grade 3 bladder cancer than in grade 1, and was higher in advanced than in early stage cancer. CONCLUSIONS: These results demonstrate that generated cyclooxygenase-2 in the cells of patients with bladder cancer might be significant in the proliferation of bladder malignant cells and development of invasions. SN - 0022-5347 UR - https://www.unboundmedicine.com/medline/citation/11342898/Expression_of_cyclooxygenase_2_in_patients_with_bladder_carcinoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-5347(05)66329-X DB - PRIME DP - Unbound Medicine ER -