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Allergen-induced changes in airway responsiveness are not related to indices of airway edema.
J Allergy Clin Immunol. 2001 May; 107(5):805-11.JA

Abstract

BACKGROUND

The mechanisms behind airway hyperresponsiveness in asthma are unknown. Airway wall edema has been proposed as one possible culprit of this phenomenon.

OBJECTIVE

To test the hypothesis that airway edema may be the cause of allergen-induced increases in airway responsiveness in asthma, this trial aimed at determining the relationship between allergen-induced changes in airway responsiveness to inhaled methacholine and indirect indices of edema, namely peripheral airway resistance and the levels of the plasma protein fibrinogen in bronchoalveolar lavage (BAL) fluids.

METHODS

Twenty-six atopic individuals with mild asthma were subjected to bronchoscopy at baseline and 28 hours after allergen inhalation. Before each bronchoscopy, methacholine bronchoprovocation was performed. During bronchoscopy, peripheral airway resistance measurements were obtained by wedged bronchoscopy. BAL fluids were analyzed for fibrinogen, as well as for eosinophilic cationic protein. Cytology was performed, and cytokine gene expression was assessed with competitive reverse transcriptase PCR from cell pellets.

RESULTS

A significant increase in airway responsiveness to methacholine was recorded after allergen, but this did not correlate with changes in peripheral airway resistance (which was not affected) or with BAL fibrinogen (which decreased after allergen). Other BAL outcomes confirmed that airway inflammation was produced and was characterized by a T(H)2 cytokine pattern.

CONCLUSIONS

Airway responsiveness in asthma increases after exposure to allergen in the absence of increased indirect indices of edema. The role of edema in this phenomenon should therefore be tested more vigorously.

Authors+Show Affiliations

Division of Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11344346

Citation

Peebles, R S., et al. "Allergen-induced Changes in Airway Responsiveness Are Not Related to Indices of Airway Edema." The Journal of Allergy and Clinical Immunology, vol. 107, no. 5, 2001, pp. 805-11.
Peebles RS, Wagner EM, Liu MC, et al. Allergen-induced changes in airway responsiveness are not related to indices of airway edema. J Allergy Clin Immunol. 2001;107(5):805-11.
Peebles, R. S., Wagner, E. M., Liu, M. C., Proud, D., Hamilton, R. G., & Togias, A. (2001). Allergen-induced changes in airway responsiveness are not related to indices of airway edema. The Journal of Allergy and Clinical Immunology, 107(5), 805-11.
Peebles RS, et al. Allergen-induced Changes in Airway Responsiveness Are Not Related to Indices of Airway Edema. J Allergy Clin Immunol. 2001;107(5):805-11. PubMed PMID: 11344346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Allergen-induced changes in airway responsiveness are not related to indices of airway edema. AU - Peebles,R S,Jr AU - Wagner,E M, AU - Liu,M C, AU - Proud,D, AU - Hamilton,R G, AU - Togias,A, PY - 2001/5/10/pubmed PY - 2001/6/22/medline PY - 2001/5/10/entrez SP - 805 EP - 11 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 107 IS - 5 N2 - BACKGROUND: The mechanisms behind airway hyperresponsiveness in asthma are unknown. Airway wall edema has been proposed as one possible culprit of this phenomenon. OBJECTIVE: To test the hypothesis that airway edema may be the cause of allergen-induced increases in airway responsiveness in asthma, this trial aimed at determining the relationship between allergen-induced changes in airway responsiveness to inhaled methacholine and indirect indices of edema, namely peripheral airway resistance and the levels of the plasma protein fibrinogen in bronchoalveolar lavage (BAL) fluids. METHODS: Twenty-six atopic individuals with mild asthma were subjected to bronchoscopy at baseline and 28 hours after allergen inhalation. Before each bronchoscopy, methacholine bronchoprovocation was performed. During bronchoscopy, peripheral airway resistance measurements were obtained by wedged bronchoscopy. BAL fluids were analyzed for fibrinogen, as well as for eosinophilic cationic protein. Cytology was performed, and cytokine gene expression was assessed with competitive reverse transcriptase PCR from cell pellets. RESULTS: A significant increase in airway responsiveness to methacholine was recorded after allergen, but this did not correlate with changes in peripheral airway resistance (which was not affected) or with BAL fibrinogen (which decreased after allergen). Other BAL outcomes confirmed that airway inflammation was produced and was characterized by a T(H)2 cytokine pattern. CONCLUSIONS: Airway responsiveness in asthma increases after exposure to allergen in the absence of increased indirect indices of edema. The role of edema in this phenomenon should therefore be tested more vigorously. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/11344346/Allergen_induced_changes_in_airway_responsiveness_are_not_related_to_indices_of_airway_edema_ DB - PRIME DP - Unbound Medicine ER -