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The differential hepatic uptake of chylomicron remnants of different fatty acid composition is not mediated by hepatic lipase.
Br J Nutr. 2001 May; 85(5):575-82.BJ

Abstract

The hypothesis that hepatic lipase mediates the differential hepatic uptake of chylomicron remnants of different fatty acid composition, demonstrated in previous work from our laboratory, was tested by investigating the effect of antibodies to the enzyme on the uptake of remnants enriched with saturated or n-3 polyunsaturated fatty acids by the perfused rat liver. After perfusion of rat livers with polyclonal antibodies to rat hepatic lipase raised in rabbits or with rabbit non-immune serum for 15 min, [3H]oleate-labelled chylomicron remnants, derived from chylomicrons of rats given a bolus of either palm (rich in saturated fatty acids) oil or fish (rich in n-3 polyunsaturated fatty acids) oil, were added. The disappearance of radioactivity from the perfusate during 120 min and its recovery in the liver at the end of the experiments were then measured. Although the rabbit anti-rat hepatic lipase antiserum was shown to inhibit hepatic lipase activity by up to 90%, and to bind extensively to hepatic sinusoidal surfaces when added to the perfusate, radioactivity from remnants of chylomicrons from rats given a bolus of fish oil as compared with palm oil disappeared from the perfusate and appeared in the liver more rapidly in the presence both the antiserum and the non-immune serum, and the differences between the uptake of the two types of remnants were similar. We conclude, therefore, that differential interaction with hepatic lipase is not responsible for the differences in the rate of removal of chylomicron remnants of different fatty acid composition from the blood.

Authors+Show Affiliations

Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St, London NW1 0TU, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11348572

Citation

Lambert, M S., et al. "The Differential Hepatic Uptake of Chylomicron Remnants of Different Fatty Acid Composition Is Not Mediated By Hepatic Lipase." The British Journal of Nutrition, vol. 85, no. 5, 2001, pp. 575-82.
Lambert MS, Avella MA, Berhane Y, et al. The differential hepatic uptake of chylomicron remnants of different fatty acid composition is not mediated by hepatic lipase. Br J Nutr. 2001;85(5):575-82.
Lambert, M. S., Avella, M. A., Berhane, Y., Shervill, E., & Botham, K. M. (2001). The differential hepatic uptake of chylomicron remnants of different fatty acid composition is not mediated by hepatic lipase. The British Journal of Nutrition, 85(5), 575-82.
Lambert MS, et al. The Differential Hepatic Uptake of Chylomicron Remnants of Different Fatty Acid Composition Is Not Mediated By Hepatic Lipase. Br J Nutr. 2001;85(5):575-82. PubMed PMID: 11348572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The differential hepatic uptake of chylomicron remnants of different fatty acid composition is not mediated by hepatic lipase. AU - Lambert,M S, AU - Avella,M A, AU - Berhane,Y, AU - Shervill,E, AU - Botham,K M, PY - 2001/5/12/pubmed PY - 2001/6/8/medline PY - 2001/5/12/entrez SP - 575 EP - 82 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 85 IS - 5 N2 - The hypothesis that hepatic lipase mediates the differential hepatic uptake of chylomicron remnants of different fatty acid composition, demonstrated in previous work from our laboratory, was tested by investigating the effect of antibodies to the enzyme on the uptake of remnants enriched with saturated or n-3 polyunsaturated fatty acids by the perfused rat liver. After perfusion of rat livers with polyclonal antibodies to rat hepatic lipase raised in rabbits or with rabbit non-immune serum for 15 min, [3H]oleate-labelled chylomicron remnants, derived from chylomicrons of rats given a bolus of either palm (rich in saturated fatty acids) oil or fish (rich in n-3 polyunsaturated fatty acids) oil, were added. The disappearance of radioactivity from the perfusate during 120 min and its recovery in the liver at the end of the experiments were then measured. Although the rabbit anti-rat hepatic lipase antiserum was shown to inhibit hepatic lipase activity by up to 90%, and to bind extensively to hepatic sinusoidal surfaces when added to the perfusate, radioactivity from remnants of chylomicrons from rats given a bolus of fish oil as compared with palm oil disappeared from the perfusate and appeared in the liver more rapidly in the presence both the antiserum and the non-immune serum, and the differences between the uptake of the two types of remnants were similar. We conclude, therefore, that differential interaction with hepatic lipase is not responsible for the differences in the rate of removal of chylomicron remnants of different fatty acid composition from the blood. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/11348572/The_differential_hepatic_uptake_of_chylomicron_remnants_of_different_fatty_acid_composition_is_not_mediated_by_hepatic_lipase_ L2 - https://www.cambridge.org/core/product/identifier/S0007114501000824/type/journal_article DB - PRIME DP - Unbound Medicine ER -