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Characterization of the manganese-containing superoxide dismutase and its gene regulation in stress response of Schizosaccharomyces pombe.
Biochem Biophys Res Commun. 2001 May 18; 283(4):908-14.BB

Abstract

Fission yeast Schizosaccharomyces pombe contains two superoxide dismutases (SODs), one in the cytosol and the other in mitochondria. The sod2+ gene encoding putative mitochondrial superoxide dismutase containing manganese (MnSOD) has been isolated. Purification and analysis of the sod2+ gene product revealed that it contained only manganese as a cofactor, thus verified to be a genuine MnSOD. It was localized in mitochondria as expected. Its N-terminal amino acid sequence indicated that the mitochondrial targeting sequence of 21 amino acids was removed. The native form consisted of two identical subunits. The sod2+ expression was induced by external stresses, such as treatments with superoxide generators, high osmolarity, and heat. The induction by these stress treatments depended on Wis1-Spc1 MAPK signal transduction pathway being independent of transcription factors Atf1 or Pap1. The sod2 disruption rendered cells sensitive to various superoxide-generators, heat, and high osmolarity, suggesting that the mitochondrial MnSOD acts as a general defense agent against multiple stresses.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Seoul National University, Seoul, 151-742, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11350071

Citation

Jeong, J H., et al. "Characterization of the Manganese-containing Superoxide Dismutase and Its Gene Regulation in Stress Response of Schizosaccharomyces Pombe." Biochemical and Biophysical Research Communications, vol. 283, no. 4, 2001, pp. 908-14.
Jeong JH, Kwon ES, Roe JH. Characterization of the manganese-containing superoxide dismutase and its gene regulation in stress response of Schizosaccharomyces pombe. Biochem Biophys Res Commun. 2001;283(4):908-14.
Jeong, J. H., Kwon, E. S., & Roe, J. H. (2001). Characterization of the manganese-containing superoxide dismutase and its gene regulation in stress response of Schizosaccharomyces pombe. Biochemical and Biophysical Research Communications, 283(4), 908-14.
Jeong JH, Kwon ES, Roe JH. Characterization of the Manganese-containing Superoxide Dismutase and Its Gene Regulation in Stress Response of Schizosaccharomyces Pombe. Biochem Biophys Res Commun. 2001 May 18;283(4):908-14. PubMed PMID: 11350071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the manganese-containing superoxide dismutase and its gene regulation in stress response of Schizosaccharomyces pombe. AU - Jeong,J H, AU - Kwon,E S, AU - Roe,J H, PY - 2001/5/15/pubmed PY - 2001/6/22/medline PY - 2001/5/15/entrez SP - 908 EP - 14 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 283 IS - 4 N2 - Fission yeast Schizosaccharomyces pombe contains two superoxide dismutases (SODs), one in the cytosol and the other in mitochondria. The sod2+ gene encoding putative mitochondrial superoxide dismutase containing manganese (MnSOD) has been isolated. Purification and analysis of the sod2+ gene product revealed that it contained only manganese as a cofactor, thus verified to be a genuine MnSOD. It was localized in mitochondria as expected. Its N-terminal amino acid sequence indicated that the mitochondrial targeting sequence of 21 amino acids was removed. The native form consisted of two identical subunits. The sod2+ expression was induced by external stresses, such as treatments with superoxide generators, high osmolarity, and heat. The induction by these stress treatments depended on Wis1-Spc1 MAPK signal transduction pathway being independent of transcription factors Atf1 or Pap1. The sod2 disruption rendered cells sensitive to various superoxide-generators, heat, and high osmolarity, suggesting that the mitochondrial MnSOD acts as a general defense agent against multiple stresses. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/11350071/Characterization_of_the_manganese_containing_superoxide_dismutase_and_its_gene_regulation_in_stress_response_of_Schizosaccharomyces_pombe_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(01)94853-3 DB - PRIME DP - Unbound Medicine ER -