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Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats.
Histochem J 2001; 33(1):25-35HJ

Abstract

It is not known how gene expression of bone extracellular matrix molecules is controlled temporally and spatially, or how it is related with morphological differentiation of osteoblasts during embryonic osteogenesis in vivo. The present study was designed to examine gene expressions of type I collagen, osteonectin, bone sialoprotein, osteopontin, and osteocalcin during mandibular osteogenesis using in situ hybridization. Wistar rat embryos 13-20 days post coitum were used. The condensation of mesenchymal cells was formed in 14-day rat embryonic mandibles and expressed genes of pro-alpha 1 (I) collagen, osteonectin, bone sialoprotein and osteopontin. Cuboidal osteoblasts surrounding the uncalcified bone matrix were seen as early as in 15-day embryonic mandibles, while flat osteoblasts lining the surface of the calcified bone were seen from 16-day embryonic mandibles. Cuboidal osteoblasts expressed pro-alpha 1(I) collagen, osteonectin and bone sialoprotein intensely but osteopontin very weakly. In contrast, flat osteoblasts expressed osteopontin very strongly. Osteocytes expressed the extracellular matrix molecules actively, in particular, osteopontin. The present study demonstrated the distinct gene expression pattern of type I collagen, osteonectin, bone sialoprotein, osteopontin and osteocalcin during embryonic mandibular osteogenesis in vivo.

Authors+Show Affiliations

Division of Oral Molecular Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11352398

Citation

Zhu, J X., et al. "Temporal and Spatial Gene Expression of Major Bone Extracellular Matrix Molecules During Embryonic Mandibular Osteogenesis in Rats." The Histochemical Journal, vol. 33, no. 1, 2001, pp. 25-35.
Zhu JX, Sasano Y, Takahashi I, et al. Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. Histochem J. 2001;33(1):25-35.
Zhu, J. X., Sasano, Y., Takahashi, I., Mizoguchi, I., & Kagayama, M. (2001). Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. The Histochemical Journal, 33(1), pp. 25-35.
Zhu JX, et al. Temporal and Spatial Gene Expression of Major Bone Extracellular Matrix Molecules During Embryonic Mandibular Osteogenesis in Rats. Histochem J. 2001;33(1):25-35. PubMed PMID: 11352398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. AU - Zhu,J X, AU - Sasano,Y, AU - Takahashi,I, AU - Mizoguchi,I, AU - Kagayama,M, PY - 2001/5/16/pubmed PY - 2001/9/28/medline PY - 2001/5/16/entrez SP - 25 EP - 35 JF - The Histochemical journal JO - Histochem. J. VL - 33 IS - 1 N2 - It is not known how gene expression of bone extracellular matrix molecules is controlled temporally and spatially, or how it is related with morphological differentiation of osteoblasts during embryonic osteogenesis in vivo. The present study was designed to examine gene expressions of type I collagen, osteonectin, bone sialoprotein, osteopontin, and osteocalcin during mandibular osteogenesis using in situ hybridization. Wistar rat embryos 13-20 days post coitum were used. The condensation of mesenchymal cells was formed in 14-day rat embryonic mandibles and expressed genes of pro-alpha 1 (I) collagen, osteonectin, bone sialoprotein and osteopontin. Cuboidal osteoblasts surrounding the uncalcified bone matrix were seen as early as in 15-day embryonic mandibles, while flat osteoblasts lining the surface of the calcified bone were seen from 16-day embryonic mandibles. Cuboidal osteoblasts expressed pro-alpha 1(I) collagen, osteonectin and bone sialoprotein intensely but osteopontin very weakly. In contrast, flat osteoblasts expressed osteopontin very strongly. Osteocytes expressed the extracellular matrix molecules actively, in particular, osteopontin. The present study demonstrated the distinct gene expression pattern of type I collagen, osteonectin, bone sialoprotein, osteopontin and osteocalcin during embryonic mandibular osteogenesis in vivo. SN - 0018-2214 UR - https://www.unboundmedicine.com/medline/citation/11352398/Temporal_and_spatial_gene_expression_of_major_bone_extracellular_matrix_molecules_during_embryonic_mandibular_osteogenesis_in_rats_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11352398.ui DB - PRIME DP - Unbound Medicine ER -