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In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats.

Abstract

Reactive oxygen species (ROS) may be involved in the toxicity of chlorpyrifos-ethyl (CE) [O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate]. We have, therefore, examined the in vivo effects of CE on the rat erythrocyte antioxidant system and evaluated the ameliorating effects of melatonin and a combination of vitamin E and vitamin C on the oxidative damage induced by CE. The experimental groups were: (1) control group, (2) CE-treated group (CE), (3) vitamin E plus vitamin C treatment group (Vit), (4) melatonin-treated group (Mel), (5) vitamin E plus vitamin C plus CE treatment group (Vit + CE), and (6) melatonin plus CE treatment group (Mel + CE). Vitamin E and vitamin C were administered intramuscularly once a day for 6 consecutive days at 150 and 200 mg/kg, respectively, in the Vit and Vit + CE groups. Melatonin was administered intramuscularly at 10 mg/kg per day for 6 consecutive days in the Mel and Mel + CE groups. At the end of the fifth day, the rats of CE, Vit + CE and Mel + CE groups were treated orally with the first of two equal doses of 41 mg/kg CE, the second oral dose being given 21 h later. Blood samples were taken 24 h after the first CE administration. Levels of thiobarbituric acid reactive substance (TBARS), antioxidant defence potential (AOP), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were determined in erythrocytes. In comparison with the control group, oral administration of CE significantly (P < 0.05) stimulated TBARS activity while significantly (P < 0.05) inhibiting AOP and the activities of SOD and CAT. However, GSH-Px activity remained unchanged by CE treatment. Treatment with melatonin and vitamins E plus C significantly (P < 0.05) reduced the CE-induced increase of TBARS, and overcame the inhibitory effect of CE on SOD and CAT, but not on AOP. Melatonin treatment significantly (P < 0.05) increased only GSH-Px activity, irrespective of the effect of CE. These results suggest that CE treatment increases in vivo lipid peroxidation and decreases antioxidant defence by increasing oxidative stress in erythrocytes of rats, and melatonin and a combination of vitamin E and vitamin C can reduce this lipoperoxidative effect.

Authors+Show Affiliations

,

Department of Biochemistry and Clinical Biochemistry, Suleyman Demirel University, School of Medicine, 32040 Isparta, Turkey. drfatih2000@hotmail.com

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Source

Archives of toxicology 75:2 2001 Apr pg 88-96

MeSH

Administration, Oral
Animals
Antioxidants
Ascorbic Acid
Catalase
Dose-Response Relationship, Drug
Drug Therapy, Combination
Erythrocytes
Glutathione Peroxidase
Injections, Intramuscular
Insecticides
Lipid Peroxidation
Male
Melatonin
Organothiophosphorus Compounds
Oxidative Stress
Rats
Rats, Wistar
Superoxide Dismutase
Thiobarbituric Acid Reactive Substances
Vitamin D

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11354911

Citation

Gultekin, F, et al. "In Vivo Changes in Antioxidant Systems and Protective Role of Melatonin and a Combination of Vitamin C and Vitamin E On Oxidative Damage in Erythrocytes Induced By Chlorpyrifos-ethyl in Rats." Archives of Toxicology, vol. 75, no. 2, 2001, pp. 88-96.
Gultekin F, Delibas N, Yasar S, et al. In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol. 2001;75(2):88-96.
Gultekin, F., Delibas, N., Yasar, S., & Kilinc, I. (2001). In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Archives of Toxicology, 75(2), pp. 88-96.
Gultekin F, et al. In Vivo Changes in Antioxidant Systems and Protective Role of Melatonin and a Combination of Vitamin C and Vitamin E On Oxidative Damage in Erythrocytes Induced By Chlorpyrifos-ethyl in Rats. Arch Toxicol. 2001;75(2):88-96. PubMed PMID: 11354911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. AU - Gultekin,F, AU - Delibas,N, AU - Yasar,S, AU - Kilinc,I, PY - 2001/5/17/pubmed PY - 2001/9/28/medline PY - 2001/5/17/entrez SP - 88 EP - 96 JF - Archives of toxicology JO - Arch. Toxicol. VL - 75 IS - 2 N2 - Reactive oxygen species (ROS) may be involved in the toxicity of chlorpyrifos-ethyl (CE) [O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate]. We have, therefore, examined the in vivo effects of CE on the rat erythrocyte antioxidant system and evaluated the ameliorating effects of melatonin and a combination of vitamin E and vitamin C on the oxidative damage induced by CE. The experimental groups were: (1) control group, (2) CE-treated group (CE), (3) vitamin E plus vitamin C treatment group (Vit), (4) melatonin-treated group (Mel), (5) vitamin E plus vitamin C plus CE treatment group (Vit + CE), and (6) melatonin plus CE treatment group (Mel + CE). Vitamin E and vitamin C were administered intramuscularly once a day for 6 consecutive days at 150 and 200 mg/kg, respectively, in the Vit and Vit + CE groups. Melatonin was administered intramuscularly at 10 mg/kg per day for 6 consecutive days in the Mel and Mel + CE groups. At the end of the fifth day, the rats of CE, Vit + CE and Mel + CE groups were treated orally with the first of two equal doses of 41 mg/kg CE, the second oral dose being given 21 h later. Blood samples were taken 24 h after the first CE administration. Levels of thiobarbituric acid reactive substance (TBARS), antioxidant defence potential (AOP), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were determined in erythrocytes. In comparison with the control group, oral administration of CE significantly (P < 0.05) stimulated TBARS activity while significantly (P < 0.05) inhibiting AOP and the activities of SOD and CAT. However, GSH-Px activity remained unchanged by CE treatment. Treatment with melatonin and vitamins E plus C significantly (P < 0.05) reduced the CE-induced increase of TBARS, and overcame the inhibitory effect of CE on SOD and CAT, but not on AOP. Melatonin treatment significantly (P < 0.05) increased only GSH-Px activity, irrespective of the effect of CE. These results suggest that CE treatment increases in vivo lipid peroxidation and decreases antioxidant defence by increasing oxidative stress in erythrocytes of rats, and melatonin and a combination of vitamin E and vitamin C can reduce this lipoperoxidative effect. SN - 0340-5761 UR - https://www.unboundmedicine.com/medline/citation/11354911/In_vivo_changes_in_antioxidant_systems_and_protective_role_of_melatonin_and_a_combination_of_vitamin_C_and_vitamin_E_on_oxidative_damage_in_erythrocytes_induced_by_chlorpyrifos_ethyl_in_rats_ L2 - https://medlineplus.gov/vitaminc.html DB - PRIME DP - Unbound Medicine ER -