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Simultaneous determination of phosphorus-containing amino acid-herbicides by nonionic surfactant micellar electrokinetic chromatography with laser-induced fluorescence detection.
Electrophoresis. 2001 Apr; 22(6):1175-81.E

Abstract

The potential of micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) detection for the separation and determination of phosphorus-containing amino acid-herbicides (glufosinate and glyphosate), and aminomethylphosphonic acid (the major metabolite of glyphosate), involving derivatization with fluorescein isothiocyanate (FITC) isomer I, was investigated. Different variables that affect derivatization (pH, FITC concentration, time and temperature) and separation (pH and concentration of the buffer, kind and concentration of surfactants and applied voltage) were studied. The analysis was conducted within about 8 min and the use of the nonionic surfactant Triton X-100 improved the selectivity, thus indirectly enhancing sensitivity by shifting of the interfering peaks of the FITC excess. Dynamic ranges of 2.0-3,000 microg/L, limits of detection at microgram or submicrogram-per-liter level, and relative standard deviations from 4.7 to 6.4% were obtained. The ensuing method--nonionic surfactant MEKC-- is a useful choice for the determination of these herbicides as it provides limits of detection similar or lower than those reported by existing chromatographic alternatives without the use of an additional preconcentration technique such as solid-phase extraction. The separation of a mixture of nine FITC-derivatized amino acids, selected as target compounds, was also carried out to assess the discrimination power of the nonionic surfactant MEKC method for the analysis of closely related anionic analytes.

Authors+Show Affiliations

Molina M
Department of Analytical Chemistry and Ecology, University of Córdoba, Spain.
Silva M
No affiliation info available

MeSH

AminobutyratesChromatography, Micellar Electrokinetic CapillaryFluorescenceGlycineHerbicidesLasersMolecular StructureSurface-Active Agents

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11358144

Citation

Molina, M, and M Silva. "Simultaneous Determination of Phosphorus-containing Amino Acid-herbicides By Nonionic Surfactant Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection." Electrophoresis, vol. 22, no. 6, 2001, pp. 1175-81.
Molina M, Silva M. Simultaneous determination of phosphorus-containing amino acid-herbicides by nonionic surfactant micellar electrokinetic chromatography with laser-induced fluorescence detection. Electrophoresis. 2001;22(6):1175-81.
Molina, M., & Silva, M. (2001). Simultaneous determination of phosphorus-containing amino acid-herbicides by nonionic surfactant micellar electrokinetic chromatography with laser-induced fluorescence detection. Electrophoresis, 22(6), 1175-81.
Molina M, Silva M. Simultaneous Determination of Phosphorus-containing Amino Acid-herbicides By Nonionic Surfactant Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection. Electrophoresis. 2001;22(6):1175-81. PubMed PMID: 11358144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of phosphorus-containing amino acid-herbicides by nonionic surfactant micellar electrokinetic chromatography with laser-induced fluorescence detection. AU - Molina,M, AU - Silva,M, PY - 2001/5/19/pubmed PY - 2002/1/5/medline PY - 2001/5/19/entrez SP - 1175 EP - 81 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 6 N2 - The potential of micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) detection for the separation and determination of phosphorus-containing amino acid-herbicides (glufosinate and glyphosate), and aminomethylphosphonic acid (the major metabolite of glyphosate), involving derivatization with fluorescein isothiocyanate (FITC) isomer I, was investigated. Different variables that affect derivatization (pH, FITC concentration, time and temperature) and separation (pH and concentration of the buffer, kind and concentration of surfactants and applied voltage) were studied. The analysis was conducted within about 8 min and the use of the nonionic surfactant Triton X-100 improved the selectivity, thus indirectly enhancing sensitivity by shifting of the interfering peaks of the FITC excess. Dynamic ranges of 2.0-3,000 microg/L, limits of detection at microgram or submicrogram-per-liter level, and relative standard deviations from 4.7 to 6.4% were obtained. The ensuing method--nonionic surfactant MEKC-- is a useful choice for the determination of these herbicides as it provides limits of detection similar or lower than those reported by existing chromatographic alternatives without the use of an additional preconcentration technique such as solid-phase extraction. The separation of a mixture of nine FITC-derivatized amino acids, selected as target compounds, was also carried out to assess the discrimination power of the nonionic surfactant MEKC method for the analysis of closely related anionic analytes. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11358144/Simultaneous_determination_of_phosphorus_containing_amino_acid_herbicides_by_nonionic_surfactant_micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -