Tags

Type your tag names separated by a space and hit enter

Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro.

Abstract

Both green tea extract (GTE or tea polyphenols) and aged garlic extract (AGE) effectively inhibited in vitro dehydration of sickle red blood cells induced by K-Cl cotransport or red cell storage. For K-Cl cotransport induced by 500 mM urea, 0.3 mg/ml EGCg (epigallocatechin gallate; a major component in GTE) almost completely inhibited dehydration, and 6 mg/ml AGE inhibited dehydration to 30% of the control level. Both vitamins E and C had no effect at the level of 2 mM. Different tea extracts had different degrees of inhibition, but the inhibitory activity increased when the number of hydroxyl groups in the compounds increased. With storage of sickle cells at 4 degrees C for 6 days, the cells started to undergo spontaneous dehydration when incubated at 37 degrees C. Neither inhibitors for Ca-induced K efflux nor K-Cl cotransport could inhibit cell dehydration of stored sickle cells, but both GTE and AGE effectively inhibited it. Chloride efflux measurements using a chloride electrode demonstrated that both GTE and AGE inhibited anion transport in red blood cells. The inhibitory mechanism of these compounds may be related to anion transport inhibition, although involvement of their antioxidant activities can not yet be ruled out.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Philadelphia Biomedical Research Institute, King of Prussia, Pennsylvania 19406, USA. stohnishi@aol.com

    ,

    Source

    MeSH

    Anemia, Sickle Cell
    Beverages
    Carrier Proteins
    Dehydration
    Erythrocyte Aging
    Garlic
    Humans
    Plant Extracts
    Plants, Medicinal
    Symporters

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    11358376

    Citation

    Ohnishi, S T., et al. "Green Tea Extract and Aged Garlic Extract Inhibit Anion Transport and Sickle Cell Dehydration in Vitro." Blood Cells, Molecules & Diseases, vol. 27, no. 1, 2001, pp. 148-57.
    Ohnishi ST, Ohnishi T, Ogunmola GB. Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro. Blood Cells Mol Dis. 2001;27(1):148-57.
    Ohnishi, S. T., Ohnishi, T., & Ogunmola, G. B. (2001). Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro. Blood Cells, Molecules & Diseases, 27(1), pp. 148-57.
    Ohnishi ST, Ohnishi T, Ogunmola GB. Green Tea Extract and Aged Garlic Extract Inhibit Anion Transport and Sickle Cell Dehydration in Vitro. Blood Cells Mol Dis. 2001;27(1):148-57. PubMed PMID: 11358376.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro. AU - Ohnishi,S T, AU - Ohnishi,T, AU - Ogunmola,G B, PY - 2001/5/19/pubmed PY - 2001/10/5/medline PY - 2001/5/19/entrez SP - 148 EP - 57 JF - Blood cells, molecules & diseases JO - Blood Cells Mol. Dis. VL - 27 IS - 1 N2 - Both green tea extract (GTE or tea polyphenols) and aged garlic extract (AGE) effectively inhibited in vitro dehydration of sickle red blood cells induced by K-Cl cotransport or red cell storage. For K-Cl cotransport induced by 500 mM urea, 0.3 mg/ml EGCg (epigallocatechin gallate; a major component in GTE) almost completely inhibited dehydration, and 6 mg/ml AGE inhibited dehydration to 30% of the control level. Both vitamins E and C had no effect at the level of 2 mM. Different tea extracts had different degrees of inhibition, but the inhibitory activity increased when the number of hydroxyl groups in the compounds increased. With storage of sickle cells at 4 degrees C for 6 days, the cells started to undergo spontaneous dehydration when incubated at 37 degrees C. Neither inhibitors for Ca-induced K efflux nor K-Cl cotransport could inhibit cell dehydration of stored sickle cells, but both GTE and AGE effectively inhibited it. Chloride efflux measurements using a chloride electrode demonstrated that both GTE and AGE inhibited anion transport in red blood cells. The inhibitory mechanism of these compounds may be related to anion transport inhibition, although involvement of their antioxidant activities can not yet be ruled out. SN - 1079-9796 UR - https://www.unboundmedicine.com/medline/citation/11358376/Green_tea_extract_and_aged_garlic_extract_inhibit_anion_transport_and_sickle_cell_dehydration_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1079-9796(00)90368-1 DB - PRIME DP - Unbound Medicine ER -