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Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro.
Blood Cells Mol Dis 2001 Jan-Feb; 27(1):148-57BC

Abstract

Both green tea extract (GTE or tea polyphenols) and aged garlic extract (AGE) effectively inhibited in vitro dehydration of sickle red blood cells induced by K-Cl cotransport or red cell storage. For K-Cl cotransport induced by 500 mM urea, 0.3 mg/ml EGCg (epigallocatechin gallate; a major component in GTE) almost completely inhibited dehydration, and 6 mg/ml AGE inhibited dehydration to 30% of the control level. Both vitamins E and C had no effect at the level of 2 mM. Different tea extracts had different degrees of inhibition, but the inhibitory activity increased when the number of hydroxyl groups in the compounds increased. With storage of sickle cells at 4 degrees C for 6 days, the cells started to undergo spontaneous dehydration when incubated at 37 degrees C. Neither inhibitors for Ca-induced K efflux nor K-Cl cotransport could inhibit cell dehydration of stored sickle cells, but both GTE and AGE effectively inhibited it. Chloride efflux measurements using a chloride electrode demonstrated that both GTE and AGE inhibited anion transport in red blood cells. The inhibitory mechanism of these compounds may be related to anion transport inhibition, although involvement of their antioxidant activities can not yet be ruled out.

Authors+Show Affiliations

Philadelphia Biomedical Research Institute, King of Prussia, Pennsylvania 19406, USA. stohnishi@aol.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11358376

Citation

Ohnishi, S T., et al. "Green Tea Extract and Aged Garlic Extract Inhibit Anion Transport and Sickle Cell Dehydration in Vitro." Blood Cells, Molecules & Diseases, vol. 27, no. 1, 2001, pp. 148-57.
Ohnishi ST, Ohnishi T, Ogunmola GB. Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro. Blood Cells Mol Dis. 2001;27(1):148-57.
Ohnishi, S. T., Ohnishi, T., & Ogunmola, G. B. (2001). Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro. Blood Cells, Molecules & Diseases, 27(1), pp. 148-57.
Ohnishi ST, Ohnishi T, Ogunmola GB. Green Tea Extract and Aged Garlic Extract Inhibit Anion Transport and Sickle Cell Dehydration in Vitro. Blood Cells Mol Dis. 2001;27(1):148-57. PubMed PMID: 11358376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro. AU - Ohnishi,S T, AU - Ohnishi,T, AU - Ogunmola,G B, PY - 2001/5/19/pubmed PY - 2001/10/5/medline PY - 2001/5/19/entrez SP - 148 EP - 57 JF - Blood cells, molecules & diseases JO - Blood Cells Mol. Dis. VL - 27 IS - 1 N2 - Both green tea extract (GTE or tea polyphenols) and aged garlic extract (AGE) effectively inhibited in vitro dehydration of sickle red blood cells induced by K-Cl cotransport or red cell storage. For K-Cl cotransport induced by 500 mM urea, 0.3 mg/ml EGCg (epigallocatechin gallate; a major component in GTE) almost completely inhibited dehydration, and 6 mg/ml AGE inhibited dehydration to 30% of the control level. Both vitamins E and C had no effect at the level of 2 mM. Different tea extracts had different degrees of inhibition, but the inhibitory activity increased when the number of hydroxyl groups in the compounds increased. With storage of sickle cells at 4 degrees C for 6 days, the cells started to undergo spontaneous dehydration when incubated at 37 degrees C. Neither inhibitors for Ca-induced K efflux nor K-Cl cotransport could inhibit cell dehydration of stored sickle cells, but both GTE and AGE effectively inhibited it. Chloride efflux measurements using a chloride electrode demonstrated that both GTE and AGE inhibited anion transport in red blood cells. The inhibitory mechanism of these compounds may be related to anion transport inhibition, although involvement of their antioxidant activities can not yet be ruled out. SN - 1079-9796 UR - https://www.unboundmedicine.com/medline/citation/11358376/Green_tea_extract_and_aged_garlic_extract_inhibit_anion_transport_and_sickle_cell_dehydration_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1079-9796(00)90368-1 DB - PRIME DP - Unbound Medicine ER -