TY - JOUR T1 - Determination of phenols, flavones, and lignans in virgin olive oils by solid-phase extraction and high-performance liquid chromatography with diode array ultraviolet detection. AU - Mateos,R, AU - Espartero,J L, AU - Trujillo,M, AU - Ríos,J J, AU - León-Camacho,M, AU - Alcudia,F, AU - Cert,A, PY - 2001/5/23/pubmed PY - 2002/1/5/medline PY - 2001/5/23/entrez SP - 2185 EP - 92 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 49 IS - 5 N2 - A simple analytical method for the quantitative determination of phenols, flavones, and lignans in virgin olive oils was developed. The polar fraction was isolated from small amounts of oil sample (2.5 g) by solid-phase extraction (SPE) using diol-phase cartridges, and the extract was analyzed by reversed-phase HPLC coupled with diode array UV detection. Chromatographic separation of pinoresinol, cinnamic acid, and 1-acetoxypinoresinol was achieved. Repeatability (RSD < 6.5%), recovery (> 90%), and response factors for each identified component were determined. SPE on amino-phase cartridges was used for isolating acidic phenols and as an aid for phenol identification. For the first time, 2-(4-hydroxyphenyl)ethyl acetate was detected in olive oils. The aldehydic structure of the ligstroside aglycon was confirmed by NMR spectroscopy. The colorimetric determination of total o-diphenolic compounds by reaction with molybdate was consistent with their HPLC determination. Differences between results obtained by liquid-liquid extraction and SPE were not statistically significant. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/11368575/Determination_of_phenols_flavones_and_lignans_in_virgin_olive_oils_by_solid_phase_extraction_and_high_performance_liquid_chromatography_with_diode_array_ultraviolet_detection_ DB - PRIME DP - Unbound Medicine ER -