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Determination of gamma-hydroxybutyrate in water and human urine by solid phase microextraction-gas chromatography/quadrupole ion trap spectrometry.
J Forensic Sci. 2001 May; 46(3):688-93.JF

Abstract

A simple method of detection was developed for gamma-hydroxybutyrate (GHB). The method involves the derivatization of GHB using a hexyl-chloroformate procedure in aqueous media (such as water or urine), extraction of the derivatization product directly from the sample using solid-phase microextraction, and subsequent separation and detection with gas chromatography quadrupole ion trap mass spectrometry. The deuterated form of GHB (GHB-D6) is used as an internal standard for quantitation. The method was linear for GHB-spiked pure water samples from 2 to 150 microg/mL GHB with a detection limit of 0.2 microg/mL. Spiked urine samples showed linearity from 5 to 500 microg/mL GHB with a detection limit of 2 microg/mL. The SPME-GC/MS method is applied to actual case samples, and the results are compared to those values obtained using a conventional GC/MS method. Sensitivity and linearity are comparable to those seen using traditional methods of separation, yet the SPME method is superior due to the simplicity, speed of analysis, reduction in solvent waste, and ability to differentiate between GHB and gamma-butyrolactone (GBL).

Authors+Show Affiliations

Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11373009

Citation

Blair, S, et al. "Determination of Gamma-hydroxybutyrate in Water and Human Urine By Solid Phase Microextraction-gas Chromatography/quadrupole Ion Trap Spectrometry." Journal of Forensic Sciences, vol. 46, no. 3, 2001, pp. 688-93.
Blair S, Song M, Hall B, et al. Determination of gamma-hydroxybutyrate in water and human urine by solid phase microextraction-gas chromatography/quadrupole ion trap spectrometry. J Forensic Sci. 2001;46(3):688-93.
Blair, S., Song, M., Hall, B., & Brodbelt, J. (2001). Determination of gamma-hydroxybutyrate in water and human urine by solid phase microextraction-gas chromatography/quadrupole ion trap spectrometry. Journal of Forensic Sciences, 46(3), 688-93.
Blair S, et al. Determination of Gamma-hydroxybutyrate in Water and Human Urine By Solid Phase Microextraction-gas Chromatography/quadrupole Ion Trap Spectrometry. J Forensic Sci. 2001;46(3):688-93. PubMed PMID: 11373009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of gamma-hydroxybutyrate in water and human urine by solid phase microextraction-gas chromatography/quadrupole ion trap spectrometry. AU - Blair,S, AU - Song,M, AU - Hall,B, AU - Brodbelt,J, PY - 2001/5/25/pubmed PY - 2001/6/8/medline PY - 2001/5/25/entrez SP - 688 EP - 93 JF - Journal of forensic sciences JO - J Forensic Sci VL - 46 IS - 3 N2 - A simple method of detection was developed for gamma-hydroxybutyrate (GHB). The method involves the derivatization of GHB using a hexyl-chloroformate procedure in aqueous media (such as water or urine), extraction of the derivatization product directly from the sample using solid-phase microextraction, and subsequent separation and detection with gas chromatography quadrupole ion trap mass spectrometry. The deuterated form of GHB (GHB-D6) is used as an internal standard for quantitation. The method was linear for GHB-spiked pure water samples from 2 to 150 microg/mL GHB with a detection limit of 0.2 microg/mL. Spiked urine samples showed linearity from 5 to 500 microg/mL GHB with a detection limit of 2 microg/mL. The SPME-GC/MS method is applied to actual case samples, and the results are compared to those values obtained using a conventional GC/MS method. Sensitivity and linearity are comparable to those seen using traditional methods of separation, yet the SPME method is superior due to the simplicity, speed of analysis, reduction in solvent waste, and ability to differentiate between GHB and gamma-butyrolactone (GBL). SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/11373009/Determination_of_gamma_hydroxybutyrate_in_water_and_human_urine_by_solid_phase_microextraction_gas_chromatography/quadrupole_ion_trap_spectrometry_ L2 - https://medlineplus.gov/clubdrugs.html DB - PRIME DP - Unbound Medicine ER -