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Co-localization of vascular endothelial growth factor and its two receptors flt-1 and kdr in the mink placenta.
Placenta. 2001 May; 22(5):457-65.P

Abstract

Placental angiogenesis plays an important role in placental development and morphogenesis. Vascular endothelial growth factor (VEGF) is a well-known angiogenic growth factor, which has previously been localized in different epitheliochorial and haemochorial placenta types. In the present study VEGF and its Flt-1(VEGFR-1) and KDR (VEGFR-2) receptors were immunolocalized in the endotheliochorial mink placenta throughout gestation. VEGF, Flt-1 and KDR co-localized to fetal and maternal microvascular endothelial cells, but with a temporal difference, displaying KDR in endothelial cells throughout gestation, whereas the VEGF and Flt-1 maternal endothelial cell staining was most intense during late gestation. Additionally, KDR was found in vascular related mesenchymal cells. The VEGF-receptors were also localized in non-endothelial cells, e.g. the uterine luminal and glandular epithelium as well as the trophoblast. Our results are in agreement with former studies, showing the different effects of the Flt-1-and KDR receptors in respect of angiogenesis. More importantly, the present study of the endotheliochorial placenta localizes the VEGF-ligand-receptor system in non-endothelial cells, and thereby strengthen the hypothesis that VEGF, apart from its well-established angiogenic properties, must also have additional functional roles in the establishment and development of the placenta.

Authors+Show Affiliations

Institute of Anatomy, Physiology and Cell Biology, The Royal Veterinary and Agricultural University, Bülowsvej 13, Copenhagen, DK-1870, Denmark. hw@kvl.dkNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11373156

Citation

Winther, H, and V Dantzer. "Co-localization of Vascular Endothelial Growth Factor and Its Two Receptors Flt-1 and Kdr in the Mink Placenta." Placenta, vol. 22, no. 5, 2001, pp. 457-65.
Winther H, Dantzer V. Co-localization of vascular endothelial growth factor and its two receptors flt-1 and kdr in the mink placenta. Placenta. 2001;22(5):457-65.
Winther, H., & Dantzer, V. (2001). Co-localization of vascular endothelial growth factor and its two receptors flt-1 and kdr in the mink placenta. Placenta, 22(5), 457-65.
Winther H, Dantzer V. Co-localization of Vascular Endothelial Growth Factor and Its Two Receptors Flt-1 and Kdr in the Mink Placenta. Placenta. 2001;22(5):457-65. PubMed PMID: 11373156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-localization of vascular endothelial growth factor and its two receptors flt-1 and kdr in the mink placenta. AU - Winther,H, AU - Dantzer,V, PY - 2001/5/25/pubmed PY - 2001/8/3/medline PY - 2001/5/25/entrez SP - 457 EP - 65 JF - Placenta JO - Placenta VL - 22 IS - 5 N2 - Placental angiogenesis plays an important role in placental development and morphogenesis. Vascular endothelial growth factor (VEGF) is a well-known angiogenic growth factor, which has previously been localized in different epitheliochorial and haemochorial placenta types. In the present study VEGF and its Flt-1(VEGFR-1) and KDR (VEGFR-2) receptors were immunolocalized in the endotheliochorial mink placenta throughout gestation. VEGF, Flt-1 and KDR co-localized to fetal and maternal microvascular endothelial cells, but with a temporal difference, displaying KDR in endothelial cells throughout gestation, whereas the VEGF and Flt-1 maternal endothelial cell staining was most intense during late gestation. Additionally, KDR was found in vascular related mesenchymal cells. The VEGF-receptors were also localized in non-endothelial cells, e.g. the uterine luminal and glandular epithelium as well as the trophoblast. Our results are in agreement with former studies, showing the different effects of the Flt-1-and KDR receptors in respect of angiogenesis. More importantly, the present study of the endotheliochorial placenta localizes the VEGF-ligand-receptor system in non-endothelial cells, and thereby strengthen the hypothesis that VEGF, apart from its well-established angiogenic properties, must also have additional functional roles in the establishment and development of the placenta. SN - 0143-4004 UR - https://www.unboundmedicine.com/medline/citation/11373156/Co_localization_of_vascular_endothelial_growth_factor_and_its_two_receptors_flt_1_and_kdr_in_the_mink_placenta_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(01)90655-1 DB - PRIME DP - Unbound Medicine ER -