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Transcription of the ibpB heat-shock gene is under control of sigma(32)- and sigma(54)-promoters, a third regulon of heat-shock response.
Biochem Biophys Res Commun. 2001 Jun 01; 284(1):57-64.BB

Abstract

The expression of the ibpAibpB heat-shock operon of Escherichia coli was found previously not to conform to the known pattern of expression of the sigma(32)-regulated operons because the rpoH gene mutation inactivating the sigma(32) protein did not abolish the ibp induction. We show here that this effect can depend partly on the sigma(54)-promoter that is inducible by heat shock, located upstream of the ibpB, the distal gene of the operon. It may also depend on a metabolic signal, postulated by others, and possibly required for the expression of the ibpAB genes. Thus, the ibpB gene can be translated from the transcript covering the whole operon starting from the sigma(32)-promoter and from the ibpB gene transcript starting from the sigma(54)-promoter. These results indicate that the ibpB gene is a second member of the sigma(54)-heat-shock regulon in E. coli besides pspA-E operon. Thus, heat-shock response involves three regulons controlled by sigma(32), sigma(24), and sigma(54) RNA polymerase subunits.

Authors+Show Affiliations

Department of Biochemistry, University of Gdańsk, Kladki 24, Gdańsk, 80 822, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11374870

Citation

Kuczyńska-Wisńik, D, et al. "Transcription of the ibpB Heat-shock Gene Is Under Control of Sigma(32)- and Sigma(54)-promoters, a Third Regulon of Heat-shock Response." Biochemical and Biophysical Research Communications, vol. 284, no. 1, 2001, pp. 57-64.
Kuczyńska-Wisńik D, Laskowska E, Taylor A. Transcription of the ibpB heat-shock gene is under control of sigma(32)- and sigma(54)-promoters, a third regulon of heat-shock response. Biochem Biophys Res Commun. 2001;284(1):57-64.
Kuczyńska-Wisńik, D., Laskowska, E., & Taylor, A. (2001). Transcription of the ibpB heat-shock gene is under control of sigma(32)- and sigma(54)-promoters, a third regulon of heat-shock response. Biochemical and Biophysical Research Communications, 284(1), 57-64.
Kuczyńska-Wisńik D, Laskowska E, Taylor A. Transcription of the ibpB Heat-shock Gene Is Under Control of Sigma(32)- and Sigma(54)-promoters, a Third Regulon of Heat-shock Response. Biochem Biophys Res Commun. 2001 Jun 1;284(1):57-64. PubMed PMID: 11374870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcription of the ibpB heat-shock gene is under control of sigma(32)- and sigma(54)-promoters, a third regulon of heat-shock response. AU - Kuczyńska-Wisńik,D, AU - Laskowska,E, AU - Taylor,A, PY - 2001/5/26/pubmed PY - 2001/7/20/medline PY - 2001/5/26/entrez SP - 57 EP - 64 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 284 IS - 1 N2 - The expression of the ibpAibpB heat-shock operon of Escherichia coli was found previously not to conform to the known pattern of expression of the sigma(32)-regulated operons because the rpoH gene mutation inactivating the sigma(32) protein did not abolish the ibp induction. We show here that this effect can depend partly on the sigma(54)-promoter that is inducible by heat shock, located upstream of the ibpB, the distal gene of the operon. It may also depend on a metabolic signal, postulated by others, and possibly required for the expression of the ibpAB genes. Thus, the ibpB gene can be translated from the transcript covering the whole operon starting from the sigma(32)-promoter and from the ibpB gene transcript starting from the sigma(54)-promoter. These results indicate that the ibpB gene is a second member of the sigma(54)-heat-shock regulon in E. coli besides pspA-E operon. Thus, heat-shock response involves three regulons controlled by sigma(32), sigma(24), and sigma(54) RNA polymerase subunits. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/11374870/Transcription_of_the_ibpB_heat_shock_gene_is_under_control_of_sigma_32___and_sigma_54__promoters_a_third_regulon_of_heat_shock_response_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006291X01949265 DB - PRIME DP - Unbound Medicine ER -