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p53 and K-ras mutations in pancreatic juice samples from patients with chronic pancreatitis.
Gastrointest Endosc 2001; 53(7):734-43GE

Abstract

BACKGROUND

Mutations in p53 and ras genes are frequent in pancreatic carcinoma. Several ras mutations are consistently detected in the pancreatic juice from patients with chronic pancreatitis. The p53 gene mutations have been detected occasionally in chronic pancreatitis tissue. It was the aim of this study to evaluate the presence and clinical significance of p53 and ras mutations in clinical pancreatic juice samples from patients with chronic pancreatitis.

METHODS

Pancreatic juice was obtained from 66 patients with chronic pancreatitis and no evidence of pancreatic carcinoma (51 men, 15 women; age 17-86 years [mean 49.6 +/- 12.9]). Patients were followed prospectively for 26 +/- 3 (4-54) months. Detection of p53 gene mutations was by temperature gradient gel electrophoresis (TGGE) and single strand conformation polymorphism (SSCP) for exons 5-8. Analysis of ras mutations was performed by SSCP/polymerase chain reaction, restriction fragment length polymorphism/polymerase chain reaction. All mutations were confirmed by sequencing.

RESULTS

Five of 66 (7.5%) pancreatic juice samples contained p53 mutations, and ras mutations were detected in 6 cases (9%). Cytology was negative in all cases. No pancreatic carcinoma developed during follow-up and neither cancer cells nor preneoplastic lesions could be detected histologically in resected specimens. Although no correlation between p53 mutations and duration of pancreatitis or drinking habits was found, K-ras mutations correlated with both heavy smoking and severity of the disease.

CONCLUSION

p53 and ras mutations can be detected in a minority of pancreatic juice samples from patients with chronic pancreatitis in the absence of malignancy.

Authors+Show Affiliations

Division of Gastroenterology, Department of Medicine, University of Rostock, Rostock, Germany.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11375580

Citation

Löhr, M, et al. "P53 and K-ras Mutations in Pancreatic Juice Samples From Patients With Chronic Pancreatitis." Gastrointestinal Endoscopy, vol. 53, no. 7, 2001, pp. 734-43.
Löhr M, Müller P, Mora J, et al. P53 and K-ras mutations in pancreatic juice samples from patients with chronic pancreatitis. Gastrointest Endosc. 2001;53(7):734-43.
Löhr, M., Müller, P., Mora, J., Brinkmann, B., Ostwald, C., Farré, A., ... Liebe, S. (2001). P53 and K-ras mutations in pancreatic juice samples from patients with chronic pancreatitis. Gastrointestinal Endoscopy, 53(7), pp. 734-43.
Löhr M, et al. P53 and K-ras Mutations in Pancreatic Juice Samples From Patients With Chronic Pancreatitis. Gastrointest Endosc. 2001;53(7):734-43. PubMed PMID: 11375580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - p53 and K-ras mutations in pancreatic juice samples from patients with chronic pancreatitis. AU - Löhr,M, AU - Müller,P, AU - Mora,J, AU - Brinkmann,B, AU - Ostwald,C, AU - Farré,A, AU - Lluis,F, AU - Adam,U, AU - Stubbe,J, AU - Plath,F, AU - Nizze,H, AU - Hopt,U T, AU - Barten,M, AU - Capellá,G, AU - Liebe,S, PY - 2001/5/26/pubmed PY - 2001/8/24/medline PY - 2001/5/26/entrez SP - 734 EP - 43 JF - Gastrointestinal endoscopy JO - Gastrointest. Endosc. VL - 53 IS - 7 N2 - BACKGROUND: Mutations in p53 and ras genes are frequent in pancreatic carcinoma. Several ras mutations are consistently detected in the pancreatic juice from patients with chronic pancreatitis. The p53 gene mutations have been detected occasionally in chronic pancreatitis tissue. It was the aim of this study to evaluate the presence and clinical significance of p53 and ras mutations in clinical pancreatic juice samples from patients with chronic pancreatitis. METHODS: Pancreatic juice was obtained from 66 patients with chronic pancreatitis and no evidence of pancreatic carcinoma (51 men, 15 women; age 17-86 years [mean 49.6 +/- 12.9]). Patients were followed prospectively for 26 +/- 3 (4-54) months. Detection of p53 gene mutations was by temperature gradient gel electrophoresis (TGGE) and single strand conformation polymorphism (SSCP) for exons 5-8. Analysis of ras mutations was performed by SSCP/polymerase chain reaction, restriction fragment length polymorphism/polymerase chain reaction. All mutations were confirmed by sequencing. RESULTS: Five of 66 (7.5%) pancreatic juice samples contained p53 mutations, and ras mutations were detected in 6 cases (9%). Cytology was negative in all cases. No pancreatic carcinoma developed during follow-up and neither cancer cells nor preneoplastic lesions could be detected histologically in resected specimens. Although no correlation between p53 mutations and duration of pancreatitis or drinking habits was found, K-ras mutations correlated with both heavy smoking and severity of the disease. CONCLUSION: p53 and ras mutations can be detected in a minority of pancreatic juice samples from patients with chronic pancreatitis in the absence of malignancy. SN - 0016-5107 UR - https://www.unboundmedicine.com/medline/citation/11375580/p53_and_K_ras_mutations_in_pancreatic_juice_samples_from_patients_with_chronic_pancreatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-5107(01)29815-0 DB - PRIME DP - Unbound Medicine ER -