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Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for confirmation of AMPLICOR PCR-positive samples and comparison of its diagnostic performance according to storage conditions and preparation of endocervical specimens.
J Clin Microbiol. 2001 Jun; 39(6):2280-2.JC

Abstract

The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.

Authors+Show Affiliations

STD/HIV Research and Intervention Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. evandyck@itg.beNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11376070

Citation

Van Dyck, E, et al. "Evaluation of the Roche Neisseria Gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens." Journal of Clinical Microbiology, vol. 39, no. 6, 2001, pp. 2280-2.
Van Dyck E, Smet H, Van Damme L, et al. Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for confirmation of AMPLICOR PCR-positive samples and comparison of its diagnostic performance according to storage conditions and preparation of endocervical specimens. J Clin Microbiol. 2001;39(6):2280-2.
Van Dyck, E., Smet, H., Van Damme, L., & Laga, M. (2001). Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for confirmation of AMPLICOR PCR-positive samples and comparison of its diagnostic performance according to storage conditions and preparation of endocervical specimens. Journal of Clinical Microbiology, 39(6), 2280-2.
Van Dyck E, et al. Evaluation of the Roche Neisseria Gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens. J Clin Microbiol. 2001;39(6):2280-2. PubMed PMID: 11376070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for confirmation of AMPLICOR PCR-positive samples and comparison of its diagnostic performance according to storage conditions and preparation of endocervical specimens. AU - Van Dyck,E, AU - Smet,H, AU - Van Damme,L, AU - Laga,M, PY - 2001/5/29/pubmed PY - 2001/8/24/medline PY - 2001/5/29/entrez SP - 2280 EP - 2 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 39 IS - 6 N2 - The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11376070/Evaluation_of_the_Roche_Neisseria_gonorrhoeae_16S_rRNA_PCR_for_confirmation_of_AMPLICOR_PCR_positive_samples_and_comparison_of_its_diagnostic_performance_according_to_storage_conditions_and_preparation_of_endocervical_specimens_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=11376070 DB - PRIME DP - Unbound Medicine ER -