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Association between islets of Langerhans and pancreatic ductal system in adult rat. Where endocrine and exocrine meet together?
Diabetologia. 2001 May; 44(5):575-84.D

Abstract

AIMS/HYPOTHESIS

Studies on the functional and morphological relations between exocrine and endocrine pancreas have been conducted mainly to disclose the influence of islets of Langerhans on acinar parenchyma. Less attention has been paid to the relations occurring between islets and pancreatic ducts.

METHODS

A series of consecutive sections of normal adult rat pancreas were double stained with islet (hormones) and duct (cytokeratin 20) markers. Electron microscopy was conducted to investigate the ultra-structural features of duct-islet relations and anti-insulin immunogold labelling was carried out to reveal the presence of insulin in the pancreatic duct system.

RESULTS

Consecutive double-stained sections demonstrated that 73.60 +/- 2.97% of the islets were attached to the ducts. For each series, 93.48 +/- 5.43 % of the islets contacting the duct tree were associated with small-sized ducts or centroacinar cells. Electron microscopy revealed that some insulin and somatostatin cells do face the duct lumen. Insulin was detected within the duct lumen and in the endosomal compartment of the duct cells.

CONCLUSIONS/INTERPRETATION

The finding that most islets are connected with the duct system in the adult pancreas is discussed in terms of hormone secretion into the ducts, islet histogenesis and the relation among the three tissue components of the pancreas, the endocrine, the exocrine and the duct system.

Links

Publisher Full Text

Authors+Show Affiliations

Bertelli E
Department of Biomedical Sciences, University of Siena, Italy.
Regoli M
No affiliation info available
Orazioli D
No affiliation info available
Bendayan M
No affiliation info available

MeSH

AnimalsGlucagonImmunohistochemistryIntercellular JunctionsIslets of LangerhansKeratinsMalePancreatic DuctsRatsRats, Sprague-DawleySomatostatin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11380075

Citation

Bertelli, E, et al. "Association Between Islets of Langerhans and Pancreatic Ductal System in Adult Rat. Where Endocrine and Exocrine Meet Together?" Diabetologia, vol. 44, no. 5, 2001, pp. 575-84.
Bertelli E, Regoli M, Orazioli D, et al. Association between islets of Langerhans and pancreatic ductal system in adult rat. Where endocrine and exocrine meet together? Diabetologia. 2001;44(5):575-84.
Bertelli, E., Regoli, M., Orazioli, D., & Bendayan, M. (2001). Association between islets of Langerhans and pancreatic ductal system in adult rat. Where endocrine and exocrine meet together? Diabetologia, 44(5), 575-84.
Bertelli E, et al. Association Between Islets of Langerhans and Pancreatic Ductal System in Adult Rat. Where Endocrine and Exocrine Meet Together. Diabetologia. 2001;44(5):575-84. PubMed PMID: 11380075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between islets of Langerhans and pancreatic ductal system in adult rat. Where endocrine and exocrine meet together? AU - Bertelli,E, AU - Regoli,M, AU - Orazioli,D, AU - Bendayan,M, PY - 2001/5/31/pubmed PY - 2001/10/12/medline PY - 2001/5/31/entrez SP - 575 EP - 84 JF - Diabetologia JO - Diabetologia VL - 44 IS - 5 N2 - AIMS/HYPOTHESIS: Studies on the functional and morphological relations between exocrine and endocrine pancreas have been conducted mainly to disclose the influence of islets of Langerhans on acinar parenchyma. Less attention has been paid to the relations occurring between islets and pancreatic ducts. METHODS: A series of consecutive sections of normal adult rat pancreas were double stained with islet (hormones) and duct (cytokeratin 20) markers. Electron microscopy was conducted to investigate the ultra-structural features of duct-islet relations and anti-insulin immunogold labelling was carried out to reveal the presence of insulin in the pancreatic duct system. RESULTS: Consecutive double-stained sections demonstrated that 73.60 +/- 2.97% of the islets were attached to the ducts. For each series, 93.48 +/- 5.43 % of the islets contacting the duct tree were associated with small-sized ducts or centroacinar cells. Electron microscopy revealed that some insulin and somatostatin cells do face the duct lumen. Insulin was detected within the duct lumen and in the endosomal compartment of the duct cells. CONCLUSIONS/INTERPRETATION: The finding that most islets are connected with the duct system in the adult pancreas is discussed in terms of hormone secretion into the ducts, islet histogenesis and the relation among the three tissue components of the pancreas, the endocrine, the exocrine and the duct system. SN - 0012-186X UR - https://www.unboundmedicine.com/medline/citation/11380075/Association_between_islets_of_Langerhans_and_pancreatic_ductal_system_in_adult_rat__Where_endocrine_and_exocrine_meet_together L2 - https://doi.org/10.1007/s001250051663 DB - PRIME DP - Unbound Medicine ER -