Tags

Type your tag names separated by a space and hit enter

Induction of heat shock protein 72 protects retinal ganglion cells in a rat glaucoma model.
Invest Ophthalmol Vis Sci. 2001 Jun; 42(7):1522-30.IO

Abstract

PURPOSE

To investigate whether heat shock protein (Hsp) 72 is induced in retinal ganglion cells (RGCs) in experimental rat glaucoma and whether the induction of Hsp72 by heat stress or zinc (Zn(2+)) administration can increase survival of RGCs in the model.

METHODS

Intraocular pressure (IOP) was elevated unilaterally in Wistar rats with argon laser irradiation of the trabecular meshwork 5 days after intracameral injection of india ink. Immunohistochemical staining for Hsp72 was performed. The rats with elevated IOP were treated with heat stress once a week (six rats) or intraperitoneal injection of zinc (10 mg/kg) every two weeks (six rats). Untreated rats with elevated IOP served as a control group (six rats). Quercetin, an inhibitor of Hsp expression was injected in the rats with heat stress (six rats) and zinc injection (seven rats). Subsequent to 4 weeks of IOP elevation, RGCs were counted.

RESULTS

The IOP increase compared with the contralateral eyes was 48% +/- 4% throughout the study period. Hsp72 was detected only in the eyes with elevated IOP at 1 and 2 days and was weakly detected at 1 week of IOP elevation. A single administration of zinc strongly induced Hsp72 in RGCs of rats with elevated IOP for 2 weeks. Treatment with heat stress or zinc in rats with elevated IOP increased RGC survival after 4 weeks of IOP elevation, compared with the untreated control group (P = 0.004, n = 6). Quercetin reversed the positive effect of heat stress or zinc injection on RGC survival.

CONCLUSIONS

These results demonstrate the possibility of a novel therapeutic approach to glaucoma through an enhanced induction of the endogenous heat shock response.

Authors+Show Affiliations

Departments of Ophthalmology and. Neuroscience, Jules Stein Eye Institute, University of California Los Angeles School of Medicine, 90095-7000, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11381056

Citation

Park, K H., et al. "Induction of Heat Shock Protein 72 Protects Retinal Ganglion Cells in a Rat Glaucoma Model." Investigative Ophthalmology & Visual Science, vol. 42, no. 7, 2001, pp. 1522-30.
Park KH, Cozier F, Ong OC, et al. Induction of heat shock protein 72 protects retinal ganglion cells in a rat glaucoma model. Invest Ophthalmol Vis Sci. 2001;42(7):1522-30.
Park, K. H., Cozier, F., Ong, O. C., & Caprioli, J. (2001). Induction of heat shock protein 72 protects retinal ganglion cells in a rat glaucoma model. Investigative Ophthalmology & Visual Science, 42(7), 1522-30.
Park KH, et al. Induction of Heat Shock Protein 72 Protects Retinal Ganglion Cells in a Rat Glaucoma Model. Invest Ophthalmol Vis Sci. 2001;42(7):1522-30. PubMed PMID: 11381056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of heat shock protein 72 protects retinal ganglion cells in a rat glaucoma model. AU - Park,K H, AU - Cozier,F, AU - Ong,O C, AU - Caprioli,J, PY - 2001/5/31/pubmed PY - 2001/6/29/medline PY - 2001/5/31/entrez SP - 1522 EP - 30 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 42 IS - 7 N2 - PURPOSE: To investigate whether heat shock protein (Hsp) 72 is induced in retinal ganglion cells (RGCs) in experimental rat glaucoma and whether the induction of Hsp72 by heat stress or zinc (Zn(2+)) administration can increase survival of RGCs in the model. METHODS: Intraocular pressure (IOP) was elevated unilaterally in Wistar rats with argon laser irradiation of the trabecular meshwork 5 days after intracameral injection of india ink. Immunohistochemical staining for Hsp72 was performed. The rats with elevated IOP were treated with heat stress once a week (six rats) or intraperitoneal injection of zinc (10 mg/kg) every two weeks (six rats). Untreated rats with elevated IOP served as a control group (six rats). Quercetin, an inhibitor of Hsp expression was injected in the rats with heat stress (six rats) and zinc injection (seven rats). Subsequent to 4 weeks of IOP elevation, RGCs were counted. RESULTS: The IOP increase compared with the contralateral eyes was 48% +/- 4% throughout the study period. Hsp72 was detected only in the eyes with elevated IOP at 1 and 2 days and was weakly detected at 1 week of IOP elevation. A single administration of zinc strongly induced Hsp72 in RGCs of rats with elevated IOP for 2 weeks. Treatment with heat stress or zinc in rats with elevated IOP increased RGC survival after 4 weeks of IOP elevation, compared with the untreated control group (P = 0.004, n = 6). Quercetin reversed the positive effect of heat stress or zinc injection on RGC survival. CONCLUSIONS: These results demonstrate the possibility of a novel therapeutic approach to glaucoma through an enhanced induction of the endogenous heat shock response. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/11381056/Induction_of_heat_shock_protein_72_protects_retinal_ganglion_cells_in_a_rat_glaucoma_model_ L2 - https://iovs.arvojournals.org/article.aspx?volume=42&issue=7&page=1522 DB - PRIME DP - Unbound Medicine ER -