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Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal spermatozoa-ZP binding.
Hum Reprod 2001; 16(6):1185-90HR

Abstract

Results of zona pellucida (ZP)-induced acrosome reaction (AR) are reported for 186 normospermic men with unexplained infertility and compared with 34 normal fertile men and 54 patients with disordered ZP-induced AR (DZPIAR) diagnosed after failure of standard IVF. For each ZP-induced AR test, four oocytes that failed to fertilize in IVF were incubated for 2 h with 2x10(6)/ml motile spermatozoa. Spermatozoa tightly bound to the ZP were recovered by aspirating the oocytes with a pipette and the AR assessed using pisum sativum agglutinin labelled with fluorescein. The standard deviation of the difference was 5.2% for repeated tests for ZP-induced AR on different ejaculates from 54 men. The ranges for the ZP-induced AR were 3-98% for normospermic infertile men, 24-95% for fertile men and 0-16% for DZPIAR patients. In the normospermic group, there was a significant correlation between ZP-induced AR and sperm concentration (Spearman r = 0.238, P < 0.001). Using ZP-induced AR < or =16% as the threshold for diagnosis of DZPIAR, the frequency of this condition in normospermic infertile men would be 25%. Thus DZPIAR is common with normospermic idiopathic infertility and this condition should be diagnosed before assisted reproductive technology since it requires intracytoplasmic sperm injection.

Authors+Show Affiliations

University of Melbourne Department of Obstetrics and Gynaecology, Reproductive Services, Andrology Laboratory, Royal Women's Hospital and Melbourne IVF, Victoria 3053, Australia. dyl@unimelb.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11387290

Citation

Liu, D Y., et al. "Frequency of Disordered Zona Pellucida (ZP)-induced Acrosome Reaction in Infertile Men With Normal Semen Analysis and Normal spermatozoa-ZP Binding." Human Reproduction (Oxford, England), vol. 16, no. 6, 2001, pp. 1185-90.
Liu DY, Clarke GN, Martic M, et al. Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal spermatozoa-ZP binding. Hum Reprod. 2001;16(6):1185-90.
Liu, D. Y., Clarke, G. N., Martic, M., Garrett, C., & Baker, H. W. (2001). Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal spermatozoa-ZP binding. Human Reproduction (Oxford, England), 16(6), pp. 1185-90.
Liu DY, et al. Frequency of Disordered Zona Pellucida (ZP)-induced Acrosome Reaction in Infertile Men With Normal Semen Analysis and Normal spermatozoa-ZP Binding. Hum Reprod. 2001;16(6):1185-90. PubMed PMID: 11387290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal spermatozoa-ZP binding. AU - Liu,D Y, AU - Clarke,G N, AU - Martic,M, AU - Garrett,C, AU - Baker,H W, PY - 2001/6/2/pubmed PY - 2001/8/24/medline PY - 2001/6/2/entrez SP - 1185 EP - 90 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 16 IS - 6 N2 - Results of zona pellucida (ZP)-induced acrosome reaction (AR) are reported for 186 normospermic men with unexplained infertility and compared with 34 normal fertile men and 54 patients with disordered ZP-induced AR (DZPIAR) diagnosed after failure of standard IVF. For each ZP-induced AR test, four oocytes that failed to fertilize in IVF were incubated for 2 h with 2x10(6)/ml motile spermatozoa. Spermatozoa tightly bound to the ZP were recovered by aspirating the oocytes with a pipette and the AR assessed using pisum sativum agglutinin labelled with fluorescein. The standard deviation of the difference was 5.2% for repeated tests for ZP-induced AR on different ejaculates from 54 men. The ranges for the ZP-induced AR were 3-98% for normospermic infertile men, 24-95% for fertile men and 0-16% for DZPIAR patients. In the normospermic group, there was a significant correlation between ZP-induced AR and sperm concentration (Spearman r = 0.238, P < 0.001). Using ZP-induced AR < or =16% as the threshold for diagnosis of DZPIAR, the frequency of this condition in normospermic infertile men would be 25%. Thus DZPIAR is common with normospermic idiopathic infertility and this condition should be diagnosed before assisted reproductive technology since it requires intracytoplasmic sperm injection. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/11387290/Frequency_of_disordered_zona_pellucida__ZP__induced_acrosome_reaction_in_infertile_men_with_normal_semen_analysis_and_normal_spermatozoa_ZP_binding_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/16.6.1185 DB - PRIME DP - Unbound Medicine ER -