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Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers.
Biosci Biotechnol Biochem. 2001 Apr; 65(4):743-51.BB

Abstract

The effects of medium-chain fatty acids (MCFA) on intracellular calcium (Ca2+) levels and actin filaments in the Caco-2 monolayer were investigated. A site-dependent increase in intracellular Ca2+ levels caused by decanoic acid (C10) at 13 mM was observed by confocal laser scanning microscopy. The area in which the intracellular Ca2+ levels was increased was measured by image analysis, and increased to 11% of the total area of the monolayer within 1 minute. This was maintained for 5 minutes, and decreased thereafter. The other MCFAs did not significantly increase the intracellular Ca2+ levels. Obvious morphological changes of actin filaments were induced by only C10 among C8-C14. The area in which actin filaments were depleted was also quantified, and the increase in area became significant after 40 minutes. The area of the actin-depleted spot corresponded to the area occupied by 5 to 10 cells as well as that in which the intracellular Ca2+ level was increased. The effectiveness of only C10 suggested that the mechanism of the absorption enhancement by C10 would be different from that by the other MCFAs, or that C10 has some additional physiological functions although the mechanism of the enhancement is the same as for the other MCFAs.

Authors+Show Affiliations

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan. kimura@kais.kyoto-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11388448

Citation

Kimura, Y, et al. "Effects of Medium-chain Fatty Acids On Intracellular Calcium Levels and the Cytoskeleton in Human Intestinal (Caco-2) Cell Monolayers." Bioscience, Biotechnology, and Biochemistry, vol. 65, no. 4, 2001, pp. 743-51.
Kimura Y, Hosoda Y, Yamaguchi M, et al. Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers. Biosci Biotechnol Biochem. 2001;65(4):743-51.
Kimura, Y., Hosoda, Y., Yamaguchi, M., Nagano, H., Shima, M., Adachi, S., & Matsuno, R. (2001). Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers. Bioscience, Biotechnology, and Biochemistry, 65(4), 743-51.
Kimura Y, et al. Effects of Medium-chain Fatty Acids On Intracellular Calcium Levels and the Cytoskeleton in Human Intestinal (Caco-2) Cell Monolayers. Biosci Biotechnol Biochem. 2001;65(4):743-51. PubMed PMID: 11388448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers. AU - Kimura,Y, AU - Hosoda,Y, AU - Yamaguchi,M, AU - Nagano,H, AU - Shima,M, AU - Adachi,S, AU - Matsuno,R, PY - 2001/6/5/pubmed PY - 2001/10/26/medline PY - 2001/6/5/entrez SP - 743 EP - 51 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 65 IS - 4 N2 - The effects of medium-chain fatty acids (MCFA) on intracellular calcium (Ca2+) levels and actin filaments in the Caco-2 monolayer were investigated. A site-dependent increase in intracellular Ca2+ levels caused by decanoic acid (C10) at 13 mM was observed by confocal laser scanning microscopy. The area in which the intracellular Ca2+ levels was increased was measured by image analysis, and increased to 11% of the total area of the monolayer within 1 minute. This was maintained for 5 minutes, and decreased thereafter. The other MCFAs did not significantly increase the intracellular Ca2+ levels. Obvious morphological changes of actin filaments were induced by only C10 among C8-C14. The area in which actin filaments were depleted was also quantified, and the increase in area became significant after 40 minutes. The area of the actin-depleted spot corresponded to the area occupied by 5 to 10 cells as well as that in which the intracellular Ca2+ level was increased. The effectiveness of only C10 suggested that the mechanism of the absorption enhancement by C10 would be different from that by the other MCFAs, or that C10 has some additional physiological functions although the mechanism of the enhancement is the same as for the other MCFAs. SN - 0916-8451 UR - https://www.unboundmedicine.com/medline/citation/11388448/Effects_of_medium_chain_fatty_acids_on_intracellular_calcium_levels_and_the_cytoskeleton_in_human_intestinal__Caco_2__cell_monolayers_ L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/bbb/65.743?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -