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Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG, and vir box from the same Ti plasmid.
J Bacteriol. 2001 Jul; 183(13):4079-89.JB

Abstract

The vir genes of octopine, nopaline, and L,L-succinamopine Ti plasmids exhibit structural and functional similarities. However, we observed differences in the interactions between octopine and nopaline vir components. The induction of an octopine virE(A6)::lacZ fusion (pSM358cd) was 2.3-fold higher in an octopine strain (A348) than in a nopaline strain (C58). Supplementation of the octopine virG(A6) in a nopaline strain with pSM358 did not completely restore virE(A6) induction. However, addition of the octopine virA(A6) to the above strain increased virE(A6) induction to a level almost comparable to that in octopine strains. In a reciprocal analysis, the induction of a nopaline virE(C58)::cat fusion (pUCD1553) was two- to threefold higher in nopaline (C58 and T37) strains than in octopine (A348 and Ach5) and L,L-succinamopine (A281) strains. Supplementation of nopaline virA(C58) and virG(C58) in an octopine strain (A348) harboring pUCD1553 increased induction levels of virE(C58)::cat fusion to a level comparable to that in a nopaline strain (C58). Our results suggest that octopine and L,L-succinamopine VirG proteins induce the octopine virE(A6) more efficiently than they do the nopaline virE(C58). Conversely, the nopaline VirG protein induces the nopaline virE(C58) more efficiently than it does the octopine virE(A6). The ability of Bo542 virG to bring about supervirulence in tobacco is observed for an octopine vir helper (LBA4404) but not for a nopaline vir helper (PMP90). Our analyses reveal that quantitative differences exist in the interactions between VirG and vir boxes of different Ti plasmids. Efficient vir gene induction in octopine and nopaline strains requires virA, virG, and vir boxes from the respective Ti plasmids.

Authors+Show Affiliations

Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, Tamil Nadu, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11395473

Citation

Krishnamohan, A, et al. "Efficient Vir Gene Induction in Agrobacterium Tumefaciens Requires virA, virG, and Vir Box From the Same Ti Plasmid." Journal of Bacteriology, vol. 183, no. 13, 2001, pp. 4079-89.
Krishnamohan A, Balaji V, Veluthambi K. Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG, and vir box from the same Ti plasmid. J Bacteriol. 2001;183(13):4079-89.
Krishnamohan, A., Balaji, V., & Veluthambi, K. (2001). Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG, and vir box from the same Ti plasmid. Journal of Bacteriology, 183(13), 4079-89.
Krishnamohan A, Balaji V, Veluthambi K. Efficient Vir Gene Induction in Agrobacterium Tumefaciens Requires virA, virG, and Vir Box From the Same Ti Plasmid. J Bacteriol. 2001;183(13):4079-89. PubMed PMID: 11395473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG, and vir box from the same Ti plasmid. AU - Krishnamohan,A, AU - Balaji,V, AU - Veluthambi,K, PY - 2001/6/8/pubmed PY - 2001/7/13/medline PY - 2001/6/8/entrez SP - 4079 EP - 89 JF - Journal of bacteriology JO - J. Bacteriol. VL - 183 IS - 13 N2 - The vir genes of octopine, nopaline, and L,L-succinamopine Ti plasmids exhibit structural and functional similarities. However, we observed differences in the interactions between octopine and nopaline vir components. The induction of an octopine virE(A6)::lacZ fusion (pSM358cd) was 2.3-fold higher in an octopine strain (A348) than in a nopaline strain (C58). Supplementation of the octopine virG(A6) in a nopaline strain with pSM358 did not completely restore virE(A6) induction. However, addition of the octopine virA(A6) to the above strain increased virE(A6) induction to a level almost comparable to that in octopine strains. In a reciprocal analysis, the induction of a nopaline virE(C58)::cat fusion (pUCD1553) was two- to threefold higher in nopaline (C58 and T37) strains than in octopine (A348 and Ach5) and L,L-succinamopine (A281) strains. Supplementation of nopaline virA(C58) and virG(C58) in an octopine strain (A348) harboring pUCD1553 increased induction levels of virE(C58)::cat fusion to a level comparable to that in a nopaline strain (C58). Our results suggest that octopine and L,L-succinamopine VirG proteins induce the octopine virE(A6) more efficiently than they do the nopaline virE(C58). Conversely, the nopaline VirG protein induces the nopaline virE(C58) more efficiently than it does the octopine virE(A6). The ability of Bo542 virG to bring about supervirulence in tobacco is observed for an octopine vir helper (LBA4404) but not for a nopaline vir helper (PMP90). Our analyses reveal that quantitative differences exist in the interactions between VirG and vir boxes of different Ti plasmids. Efficient vir gene induction in octopine and nopaline strains requires virA, virG, and vir boxes from the respective Ti plasmids. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/11395473/Efficient_vir_gene_induction_in_Agrobacterium_tumefaciens_requires_virA_virG_and_vir_box_from_the_same_Ti_plasmid_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=11395473 DB - PRIME DP - Unbound Medicine ER -