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Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma.
J Allergy Clin Immunol. 2001 Jun; 107(6):1034-8.JA

Abstract

BACKGROUND

Angiogenesis is a feature of airway remodeling in bronchial asthma. The mechanism responsible for this angiogenesis is unknown. Vascular endothelial growth factor (VEGF) is a potent inducer of endothelial cells, which may contribute to chronic inflammation and angiogenesis.

OBJECTIVE

We sought to investigate the molecular mechanisms underlying increased vascularity, and we examined the mRNA expression of VEGF and its receptors (flt-1 and flk-1) within bronchial biopsy specimens from asthmatic patients and normal control subjects.

METHODS

Endobronchial biopsy specimens were examined immunocytochemically by staining with anti-type IV collagen mAb to evaluate vessel density by using computer-assisted image analysis. Specimens were also analyzed for the presence of the mRNAs of VEGF and its receptors with in situ hybridization.

RESULTS

The extent of airway vascularity was increased in asthmatic subjects compared with that in control subjects (P <.01). Asthmatic subjects exhibited a greater expression of VEGF, flt-1, and flk-1 mRNA(+) cells in the airway mucosa compared with that in control subjects (P <.001 for each comparison). The degree of vascularity was associated with the number of VEGF, flt-1, and flk-1 mRNA(+) cells. Numbers of cells expressing VEGF mRNA inversely correlated with airway caliber (r = -0.83, P <.01) and airway hyperresponsiveness (r = -0.97, P <.001). Colocalization studies showed that macrophages, eosinophils, and CD34(+) cells were the major sources of VEGF; CD34(+) cells, macrophages, and T cells expressed both flt-1 and flk-1.

CONCLUSION

These findings provide evidence that VEGF may play an important role in angiogenesis and subsequent airway remodeling in bronchial asthma.

Authors+Show Affiliations

Second Department of Internal Medicine, Toho University School of Medicine, 6-11-1, Omori-nishi, Ota-ku, Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11398081

Citation

Hoshino, M, et al. "Gene Expression of Vascular Endothelial Growth Factor and Its Receptors and Angiogenesis in Bronchial Asthma." The Journal of Allergy and Clinical Immunology, vol. 107, no. 6, 2001, pp. 1034-8.
Hoshino M, Nakamura Y, Hamid QA. Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma. J Allergy Clin Immunol. 2001;107(6):1034-8.
Hoshino, M., Nakamura, Y., & Hamid, Q. A. (2001). Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma. The Journal of Allergy and Clinical Immunology, 107(6), 1034-8.
Hoshino M, Nakamura Y, Hamid QA. Gene Expression of Vascular Endothelial Growth Factor and Its Receptors and Angiogenesis in Bronchial Asthma. J Allergy Clin Immunol. 2001;107(6):1034-8. PubMed PMID: 11398081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma. AU - Hoshino,M, AU - Nakamura,Y, AU - Hamid,Q A, PY - 2001/6/9/pubmed PY - 2001/7/6/medline PY - 2001/6/9/entrez SP - 1034 EP - 8 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 107 IS - 6 N2 - BACKGROUND: Angiogenesis is a feature of airway remodeling in bronchial asthma. The mechanism responsible for this angiogenesis is unknown. Vascular endothelial growth factor (VEGF) is a potent inducer of endothelial cells, which may contribute to chronic inflammation and angiogenesis. OBJECTIVE: We sought to investigate the molecular mechanisms underlying increased vascularity, and we examined the mRNA expression of VEGF and its receptors (flt-1 and flk-1) within bronchial biopsy specimens from asthmatic patients and normal control subjects. METHODS: Endobronchial biopsy specimens were examined immunocytochemically by staining with anti-type IV collagen mAb to evaluate vessel density by using computer-assisted image analysis. Specimens were also analyzed for the presence of the mRNAs of VEGF and its receptors with in situ hybridization. RESULTS: The extent of airway vascularity was increased in asthmatic subjects compared with that in control subjects (P <.01). Asthmatic subjects exhibited a greater expression of VEGF, flt-1, and flk-1 mRNA(+) cells in the airway mucosa compared with that in control subjects (P <.001 for each comparison). The degree of vascularity was associated with the number of VEGF, flt-1, and flk-1 mRNA(+) cells. Numbers of cells expressing VEGF mRNA inversely correlated with airway caliber (r = -0.83, P <.01) and airway hyperresponsiveness (r = -0.97, P <.001). Colocalization studies showed that macrophages, eosinophils, and CD34(+) cells were the major sources of VEGF; CD34(+) cells, macrophages, and T cells expressed both flt-1 and flk-1. CONCLUSION: These findings provide evidence that VEGF may play an important role in angiogenesis and subsequent airway remodeling in bronchial asthma. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/11398081/Gene_expression_of_vascular_endothelial_growth_factor_and_its_receptors_and_angiogenesis_in_bronchial_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(01)52965-6 DB - PRIME DP - Unbound Medicine ER -