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Pseudomonas aeruginosa GacA, a factor in multihost virulence, is also essential for biofilm formation.
Mol Microbiol. 2001 Jun; 40(5):1215-26.MM

Abstract

We have investigated a potential role for GacA, the response regulator of the GacA/GacS two-component regulatory system, in Pseudomonas aeruginosa biofilm formation. When gacA was disrupted in strain PA14, a 10-fold reduction in biofilm formation capacity resulted relative to wild-type PA14. However, no significant difference was observed in the planktonic growth rate of PA14 gacA(-). Providing gacA in trans on the multicopy vector pUCP-gacA abrogated the biofilm formation defect. Scanning electron microscopy of biofilms formed by PA14 gacA(-) revealed diffuse clusters of cells that failed to aggregate into microcolonies, implying a deficit in biofilm development or surface translocation. Motility assays revealed no decrease in PA14 gacA(-) twitching or swimming abilities, indicating that the defect in biofilm formation is independent of flagellar-mediated attachment and solid surface translocation by pili. Autoinducer and alginate bioassays were performed similarly, and no difference in production levels was observed, indicating that this is not merely an upstream effect on either quorum sensing or alginate production. Antibiotic susceptibility profiling demonstrated that PA14 gacA(-) biofilms have moderately decreased resistance to a range of antibiotics relative to PA14 wild type. This study establishes GacA as a new and independent regulatory element in P. aeruginosa biofilm formation.

Authors+Show Affiliations

Department of Biological Sciences, The University of Calgary, 2500 University Drive NW, Calgary AB, Canada T2N 1N4.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11401724

Citation

Parkins, M D., et al. "Pseudomonas Aeruginosa GacA, a Factor in Multihost Virulence, Is Also Essential for Biofilm Formation." Molecular Microbiology, vol. 40, no. 5, 2001, pp. 1215-26.
Parkins MD, Ceri H, Storey DG. Pseudomonas aeruginosa GacA, a factor in multihost virulence, is also essential for biofilm formation. Mol Microbiol. 2001;40(5):1215-26.
Parkins, M. D., Ceri, H., & Storey, D. G. (2001). Pseudomonas aeruginosa GacA, a factor in multihost virulence, is also essential for biofilm formation. Molecular Microbiology, 40(5), 1215-26.
Parkins MD, Ceri H, Storey DG. Pseudomonas Aeruginosa GacA, a Factor in Multihost Virulence, Is Also Essential for Biofilm Formation. Mol Microbiol. 2001;40(5):1215-26. PubMed PMID: 11401724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pseudomonas aeruginosa GacA, a factor in multihost virulence, is also essential for biofilm formation. AU - Parkins,M D, AU - Ceri,H, AU - Storey,D G, PY - 2001/6/13/pubmed PY - 2001/8/24/medline PY - 2001/6/13/entrez SP - 1215 EP - 26 JF - Molecular microbiology JO - Mol. Microbiol. VL - 40 IS - 5 N2 - We have investigated a potential role for GacA, the response regulator of the GacA/GacS two-component regulatory system, in Pseudomonas aeruginosa biofilm formation. When gacA was disrupted in strain PA14, a 10-fold reduction in biofilm formation capacity resulted relative to wild-type PA14. However, no significant difference was observed in the planktonic growth rate of PA14 gacA(-). Providing gacA in trans on the multicopy vector pUCP-gacA abrogated the biofilm formation defect. Scanning electron microscopy of biofilms formed by PA14 gacA(-) revealed diffuse clusters of cells that failed to aggregate into microcolonies, implying a deficit in biofilm development or surface translocation. Motility assays revealed no decrease in PA14 gacA(-) twitching or swimming abilities, indicating that the defect in biofilm formation is independent of flagellar-mediated attachment and solid surface translocation by pili. Autoinducer and alginate bioassays were performed similarly, and no difference in production levels was observed, indicating that this is not merely an upstream effect on either quorum sensing or alginate production. Antibiotic susceptibility profiling demonstrated that PA14 gacA(-) biofilms have moderately decreased resistance to a range of antibiotics relative to PA14 wild type. This study establishes GacA as a new and independent regulatory element in P. aeruginosa biofilm formation. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/11401724/Pseudomonas_aeruginosa_GacA_a_factor_in_multihost_virulence_is_also_essential_for_biofilm_formation_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2001&volume=40&issue=5&spage=1215 DB - PRIME DP - Unbound Medicine ER -