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Moderation of skeletal muscle reperfusion injury by a sLe(x)-glycosylated complement inhibitory protein.
Am J Physiol Cell Physiol. 2001 Jul; 281(1):C224-30.AJ

Abstract

The role of the sialyl Lewis(x) (sLe(x))-decorated version of soluble complement receptor type 1 (sCR1) in moderating skeletal muscle reperfusion injury, by antagonizing neutrophil endothelial selectin interaction and complement activation, is examined. Mice underwent 2 h of hindlimb ischemia and 3 h of reperfusion. Permeability index (PI) was assessed by extravasation of 125I-labeled albumin. Neutrophil depletion and complement inhibition with sCR1 reduced permeability by 72% (PI 0.81 +/- 0.10) compared with a 42% decrease (PI 1.53 +/- 0.08) observed in neutropenic mice, indicating that part of the complement-mediated injury is neutrophil independent. sCR1sLe(x) treatment reduced PI by 70% (PI 0.86 +/- 0.06), an additional 20% decrease compared with sCR1 treatment (PI 1.32 +/- 0.08). Treatment with sCR1sLe(x) 0.5 and 1 h after reperfusion reduced permeability by 63% (PI 0.09 +/- 0.07) and 52% (PI 1.24 +/- 0.09), respectively, compared with the respective decreases of 41% (PI 1.41 +/- 0.10) and 32% (PI 1.61 +/- 0.07) after sCR1 treatment. Muscle immunohistochemistry stained for sCR1 only on the vascular endothelium of sCR1sLe(x)-treated mice. In conclusion, sCR1sLe(x) is more effective than sCR1 in moderating skeletal muscle reperfusion injury.

Authors+Show Affiliations

Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11401845

Citation

Kyriakides, C, et al. "Moderation of Skeletal Muscle Reperfusion Injury By a sLe(x)-glycosylated Complement Inhibitory Protein." American Journal of Physiology. Cell Physiology, vol. 281, no. 1, 2001, pp. C224-30.
Kyriakides C, Wang Y, Austen WG, et al. Moderation of skeletal muscle reperfusion injury by a sLe(x)-glycosylated complement inhibitory protein. Am J Physiol Cell Physiol. 2001;281(1):C224-30.
Kyriakides, C., Wang, Y., Austen, W. G., Favuzza, J., Kobzik, L., Moore, F. D., & Hechtman, H. B. (2001). Moderation of skeletal muscle reperfusion injury by a sLe(x)-glycosylated complement inhibitory protein. American Journal of Physiology. Cell Physiology, 281(1), C224-30.
Kyriakides C, et al. Moderation of Skeletal Muscle Reperfusion Injury By a sLe(x)-glycosylated Complement Inhibitory Protein. Am J Physiol Cell Physiol. 2001;281(1):C224-30. PubMed PMID: 11401845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Moderation of skeletal muscle reperfusion injury by a sLe(x)-glycosylated complement inhibitory protein. AU - Kyriakides,C, AU - Wang,Y, AU - Austen,W G,Jr AU - Favuzza,J, AU - Kobzik,L, AU - Moore,F D,Jr AU - Hechtman,H B, PY - 2001/6/13/pubmed PY - 2001/7/13/medline PY - 2001/6/13/entrez SP - C224 EP - 30 JF - American journal of physiology. Cell physiology JO - Am J Physiol Cell Physiol VL - 281 IS - 1 N2 - The role of the sialyl Lewis(x) (sLe(x))-decorated version of soluble complement receptor type 1 (sCR1) in moderating skeletal muscle reperfusion injury, by antagonizing neutrophil endothelial selectin interaction and complement activation, is examined. Mice underwent 2 h of hindlimb ischemia and 3 h of reperfusion. Permeability index (PI) was assessed by extravasation of 125I-labeled albumin. Neutrophil depletion and complement inhibition with sCR1 reduced permeability by 72% (PI 0.81 +/- 0.10) compared with a 42% decrease (PI 1.53 +/- 0.08) observed in neutropenic mice, indicating that part of the complement-mediated injury is neutrophil independent. sCR1sLe(x) treatment reduced PI by 70% (PI 0.86 +/- 0.06), an additional 20% decrease compared with sCR1 treatment (PI 1.32 +/- 0.08). Treatment with sCR1sLe(x) 0.5 and 1 h after reperfusion reduced permeability by 63% (PI 0.09 +/- 0.07) and 52% (PI 1.24 +/- 0.09), respectively, compared with the respective decreases of 41% (PI 1.41 +/- 0.10) and 32% (PI 1.61 +/- 0.07) after sCR1 treatment. Muscle immunohistochemistry stained for sCR1 only on the vascular endothelium of sCR1sLe(x)-treated mice. In conclusion, sCR1sLe(x) is more effective than sCR1 in moderating skeletal muscle reperfusion injury. SN - 0363-6143 UR - https://www.unboundmedicine.com/medline/citation/11401845/Moderation_of_skeletal_muscle_reperfusion_injury_by_a_sLe_x__glycosylated_complement_inhibitory_protein_ L2 - https://journals.physiology.org/doi/10.1152/ajpcell.2001.281.1.C224?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -