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Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-).
Infect Immun. 2001 Jul; 69(7):4447-57.II

Abstract

Sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H(-) have emerged as important causes of diarrheal diseases and the hemolytic-uremic syndrome in Germany. In this study, we characterized a 32-kb fragment of the plasmid of SF EHEC O157:H(-), pSFO157, which differs markedly from plasmid pO157 of classical non-sorbitol-fermenting EHEC O157:H7. We found a cluster of six genes, termed sfpA, sfpH, sfpC, sfpD, sfpJ, and sfpG, which mediate mannose-resistant hemagglutination and the expression of fimbriae. sfp genes are similar to the pap genes, encoding P-fimbriae of uropathogenic E. coli, but the sfp cluster lacks homologues of genes encoding subunits of a tip fibrillum as well as regulatory genes. The major pilin, SfpA, despite its similarity to PapA, does not cluster together with known PapA alleles in a phylogenetic tree but is structurally related to the PmpA pilin of Proteus mirabilis. The putative adhesin gene sfpG, responsible for the hemagglutination phenotype, shows significant homology neither to papG nor to other known sequences. Sfp fimbriae are 3 to 5 nm in diameter, in contrast to P-fimbriae, which are 7 nm in diameter. PCR analyses showed that the sfp gene cluster is a characteristic of SF EHEC O157:H(-) strains and is not present in other EHEC isolates, diarrheagenic E. coli, or other Enterobacteriaceae. The sfp gene cluster is flanked by two blocks of insertion sequences and an origin of plasmid replication, indicating that horizontal gene transfer may have contributed to the presence of Sfp fimbriae in SF EHEC O157:H(-).

Authors+Show Affiliations

Institut für Hygiene und Mikrobiologie der Universität Würzburg, D-97080 Würzburg, Germany. wbrunder@hygiene.uni-wuerzburg.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11401985

Citation

Brunder, W, et al. "Novel Type of Fimbriae Encoded By the Large Plasmid of Sorbitol-fermenting Enterohemorrhagic Escherichia Coli O157:H(-)." Infection and Immunity, vol. 69, no. 7, 2001, pp. 4447-57.
Brunder W, Khan AS, Hacker J, et al. Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-). Infect Immun. 2001;69(7):4447-57.
Brunder, W., Khan, A. S., Hacker, J., & Karch, H. (2001). Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-). Infection and Immunity, 69(7), 4447-57.
Brunder W, et al. Novel Type of Fimbriae Encoded By the Large Plasmid of Sorbitol-fermenting Enterohemorrhagic Escherichia Coli O157:H(-). Infect Immun. 2001;69(7):4447-57. PubMed PMID: 11401985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-). AU - Brunder,W, AU - Khan,A S, AU - Hacker,J, AU - Karch,H, PY - 2001/6/13/pubmed PY - 2001/7/20/medline PY - 2001/6/13/entrez SP - 4447 EP - 57 JF - Infection and immunity JO - Infect Immun VL - 69 IS - 7 N2 - Sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H(-) have emerged as important causes of diarrheal diseases and the hemolytic-uremic syndrome in Germany. In this study, we characterized a 32-kb fragment of the plasmid of SF EHEC O157:H(-), pSFO157, which differs markedly from plasmid pO157 of classical non-sorbitol-fermenting EHEC O157:H7. We found a cluster of six genes, termed sfpA, sfpH, sfpC, sfpD, sfpJ, and sfpG, which mediate mannose-resistant hemagglutination and the expression of fimbriae. sfp genes are similar to the pap genes, encoding P-fimbriae of uropathogenic E. coli, but the sfp cluster lacks homologues of genes encoding subunits of a tip fibrillum as well as regulatory genes. The major pilin, SfpA, despite its similarity to PapA, does not cluster together with known PapA alleles in a phylogenetic tree but is structurally related to the PmpA pilin of Proteus mirabilis. The putative adhesin gene sfpG, responsible for the hemagglutination phenotype, shows significant homology neither to papG nor to other known sequences. Sfp fimbriae are 3 to 5 nm in diameter, in contrast to P-fimbriae, which are 7 nm in diameter. PCR analyses showed that the sfp gene cluster is a characteristic of SF EHEC O157:H(-) strains and is not present in other EHEC isolates, diarrheagenic E. coli, or other Enterobacteriaceae. The sfp gene cluster is flanked by two blocks of insertion sequences and an origin of plasmid replication, indicating that horizontal gene transfer may have contributed to the presence of Sfp fimbriae in SF EHEC O157:H(-). SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/11401985/Novel_type_of_fimbriae_encoded_by_the_large_plasmid_of_sorbitol_fermenting_enterohemorrhagic_Escherichia_coli_O157:H____ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=11401985 DB - PRIME DP - Unbound Medicine ER -