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Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon.
Infect Immun 2001; 69(7):4627-38II

Abstract

The complete nucleotide sequence and organization of the Yersinia enterocolitica serotype 0:8 low-calcium-response (LCR) plasmid, pYVe8081, were determined. The 67,720-bp plasmid encoded all the genes known to be part of the LCR stimulon except for ylpA. Eight of 13 intact open reading frames of unknown function identified in pYVe8081 had homologues in Yersinia pestis plasmid pCD1 or in Y. enterocolitica serotype 0:9 plasmid pYVe227. A region of approximately 17 kbp showed no DNA identity to pCD1 or pYVe227 and contained six potential new genes, a possible new replicon, and two intact insertion sequence (IS) elements. One intact IS element, ISYen1, was a new IS belonging to the IS256 family. Several vestigial IS elements appeared different from the IS distribution seen in the other LCR plasmids. The RepA proteins encoded by Y. enterocolitica serotype 0:8 pYVeWA and pYVe8081 were identical. The putative pYVe8081 replicon showed significant homology to the IncL/M replicon of pMU407.1 but was only distantly related to the replicons of pCD1 and pYVe227. In contrast, the putative partitioning genes of pYVe8081 showed 97% DNA identity to the spy/sopABC loci of pCD1 and pYVe227. Sequence analysis suggests that Yersinia LCR plasmids are from a common ancestor but that Y. enterocolitica serotype 0:8 plasmid replicons may have evolved independently via cointegrate formation following a transposition event. The change in replicon structure is predicted to change the incompatibility properties of Y. enterocolitica serotype 0:8 plasmids from those of Y. enterocolitica serotype 0:9 and Y. pestis LCR plasmids.

Authors+Show Affiliations

Department of Bacterial Diseases, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910-7500, USA. norma.snellings@na.amedd.army.milNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11402007

Citation

Snellings, N J., et al. "Complete DNA Sequence of Yersinia Enterocolitica Serotype 0:8 Low-calcium-response Plasmid Reveals a New Virulence Plasmid-associated Replicon." Infection and Immunity, vol. 69, no. 7, 2001, pp. 4627-38.
Snellings NJ, Popek M, Lindler LE. Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon. Infect Immun. 2001;69(7):4627-38.
Snellings, N. J., Popek, M., & Lindler, L. E. (2001). Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon. Infection and Immunity, 69(7), pp. 4627-38.
Snellings NJ, Popek M, Lindler LE. Complete DNA Sequence of Yersinia Enterocolitica Serotype 0:8 Low-calcium-response Plasmid Reveals a New Virulence Plasmid-associated Replicon. Infect Immun. 2001;69(7):4627-38. PubMed PMID: 11402007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon. AU - Snellings,N J, AU - Popek,M, AU - Lindler,L E, PY - 2001/6/13/pubmed PY - 2001/7/20/medline PY - 2001/6/13/entrez SP - 4627 EP - 38 JF - Infection and immunity JO - Infect. Immun. VL - 69 IS - 7 N2 - The complete nucleotide sequence and organization of the Yersinia enterocolitica serotype 0:8 low-calcium-response (LCR) plasmid, pYVe8081, were determined. The 67,720-bp plasmid encoded all the genes known to be part of the LCR stimulon except for ylpA. Eight of 13 intact open reading frames of unknown function identified in pYVe8081 had homologues in Yersinia pestis plasmid pCD1 or in Y. enterocolitica serotype 0:9 plasmid pYVe227. A region of approximately 17 kbp showed no DNA identity to pCD1 or pYVe227 and contained six potential new genes, a possible new replicon, and two intact insertion sequence (IS) elements. One intact IS element, ISYen1, was a new IS belonging to the IS256 family. Several vestigial IS elements appeared different from the IS distribution seen in the other LCR plasmids. The RepA proteins encoded by Y. enterocolitica serotype 0:8 pYVeWA and pYVe8081 were identical. The putative pYVe8081 replicon showed significant homology to the IncL/M replicon of pMU407.1 but was only distantly related to the replicons of pCD1 and pYVe227. In contrast, the putative partitioning genes of pYVe8081 showed 97% DNA identity to the spy/sopABC loci of pCD1 and pYVe227. Sequence analysis suggests that Yersinia LCR plasmids are from a common ancestor but that Y. enterocolitica serotype 0:8 plasmid replicons may have evolved independently via cointegrate formation following a transposition event. The change in replicon structure is predicted to change the incompatibility properties of Y. enterocolitica serotype 0:8 plasmids from those of Y. enterocolitica serotype 0:9 and Y. pestis LCR plasmids. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/11402007/Complete_DNA_sequence_of_Yersinia_enterocolitica_serotype_0:8_low_calcium_response_plasmid_reveals_a_new_virulence_plasmid_associated_replicon_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=11402007 DB - PRIME DP - Unbound Medicine ER -