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The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome.
Genetics. 2001 Jun; 158(2):613-25.G

Abstract

Dis3p, a subunit of the exosome, interacts directly with Ran. To clarify the relationship between the exosome and the RanGTPase cycle, a series of temperature-sensitive Saccharomyces cerevisiae dis3 mutants were isolated and their 5.8S rRNA processing was compared with processing in strains with mutations in a S. cerevisiae Ran homologue, Gsp1p. In both dis3 and gsp1 mutants, 3' processing of 7S-to-5.8S rRNA was blocked at three identical sites in an allele-specific manner. In contrast, the 5' end of 5.8S rRNA was terminated normally in gsp1 and in dis3. Inhibition of 5.8S rRNA maturation in gsp1 was rescued by overexpression of nuclear exosome components Dis3p, Rrp4p, and Mtr4p, but not by a cytoplasmic exosome component, Ski2p. Furthermore, gsp1 and dis3 accumulated the 5'-A0 fragment of 35S pre-rRNA, which is also degraded by the exosome, and the level of 27S rRNA was reduced. Neither 5.8S rRNA intermediates nor 5'-A0 fragments were observed in mutants defective in the nucleocytoplasmic transport, indicating that Gsp1p regulates rRNA processing through Dis3p, independent of nucleocytoplasmic transport.

Authors+Show Affiliations

Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3-1-1 Maedashi, Higashiku, Fukuoka 812-8582, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11404326

Citation

Suzuki, N, et al. "The Saccharomyces Cerevisiae Small GTPase, Gsp1p/Ran, Is Involved in 3' Processing of 7S-to-5.8S rRNA and in Degradation of the Excised 5'-A0 Fragment of 35S pre-rRNA, Both of Which Are Carried Out By the Exosome." Genetics, vol. 158, no. 2, 2001, pp. 613-25.
Suzuki N, Noguchi E, Nakashima N, et al. The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome. Genetics. 2001;158(2):613-25.
Suzuki, N., Noguchi, E., Nakashima, N., Oki, M., Ohba, T., Tartakoff, A., Ohishi, M., & Nishimoto, T. (2001). The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome. Genetics, 158(2), 613-25.
Suzuki N, et al. The Saccharomyces Cerevisiae Small GTPase, Gsp1p/Ran, Is Involved in 3' Processing of 7S-to-5.8S rRNA and in Degradation of the Excised 5'-A0 Fragment of 35S pre-rRNA, Both of Which Are Carried Out By the Exosome. Genetics. 2001;158(2):613-25. PubMed PMID: 11404326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome. AU - Suzuki,N, AU - Noguchi,E, AU - Nakashima,N, AU - Oki,M, AU - Ohba,T, AU - Tartakoff,A, AU - Ohishi,M, AU - Nishimoto,T, PY - 2001/6/19/pubmed PY - 2001/9/28/medline PY - 2001/6/19/entrez SP - 613 EP - 25 JF - Genetics JO - Genetics VL - 158 IS - 2 N2 - Dis3p, a subunit of the exosome, interacts directly with Ran. To clarify the relationship between the exosome and the RanGTPase cycle, a series of temperature-sensitive Saccharomyces cerevisiae dis3 mutants were isolated and their 5.8S rRNA processing was compared with processing in strains with mutations in a S. cerevisiae Ran homologue, Gsp1p. In both dis3 and gsp1 mutants, 3' processing of 7S-to-5.8S rRNA was blocked at three identical sites in an allele-specific manner. In contrast, the 5' end of 5.8S rRNA was terminated normally in gsp1 and in dis3. Inhibition of 5.8S rRNA maturation in gsp1 was rescued by overexpression of nuclear exosome components Dis3p, Rrp4p, and Mtr4p, but not by a cytoplasmic exosome component, Ski2p. Furthermore, gsp1 and dis3 accumulated the 5'-A0 fragment of 35S pre-rRNA, which is also degraded by the exosome, and the level of 27S rRNA was reduced. Neither 5.8S rRNA intermediates nor 5'-A0 fragments were observed in mutants defective in the nucleocytoplasmic transport, indicating that Gsp1p regulates rRNA processing through Dis3p, independent of nucleocytoplasmic transport. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/11404326/The_Saccharomyces_cerevisiae_small_GTPase_Gsp1p/Ran_is_involved_in_3'_processing_of_7S_to_5_8S_rRNA_and_in_degradation_of_the_excised_5'_A0_fragment_of_35S_pre_rRNA_both_of_which_are_carried_out_by_the_exosome_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1093/genetics/158.2.613 DB - PRIME DP - Unbound Medicine ER -