Tags

Type your tag names separated by a space and hit enter

Epigenetics of latent Epstein-Barr virus genomes: high resolution methylation analysis of the bidirectional promoter region of latent membrane protein 1 and 2B genes.
Biol Chem. 2001 Apr; 382(4):699-705.BC

Abstract

We analysed the methylation patterns of CpG dinucleotides in a bidirectional promoter region (LRS, LMP 1 regulatory sequences) of latent Epstein-Barr virus (EBV) genomes using automated fluorescent genomic sequencing after bisulfite-induced modification of DNA. Transcripts for two latent membrane proteins, LMP 1 (a transforming protein) and LMP 2B, are initiated in this region in opposite directions. We found that B cell lines and a clone expressing LMP 1 carried EBV genomes with unmethylated or hypomethylated LRS, while highly methylated CpG dinucleotides were present at each position or at discrete sites and within hypermethylated regions in LMP 1 negative cells. Comparison of high resolution methylation maps suggests that CpG methylation-mediated direct interference with binding of nuclear factors LBF 2, 3, 7, AML1/LBF1, LBF5 and LBF6 or methylation of CpGs within an E-box sequence (where activators as well as repressors can bind) is not the major mechanism in silencing of the LMP 1 promoter. Although a role for CpG methylation within binding sites of Sp1 and 3, ATF/CRE and a sis-inducible factor (SIF) cannot be excluded, hypermethylation of LRS or regions within LRS in LMP 1 negative cells suggests a role for an indirect mechanism, via methylcytosine binding proteins, in silencing of the LMP 1 promoter.

Authors+Show Affiliations

Division of Virology, National Center for Epidemiology, Budapest, Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11405234

Citation

Takacs, M, et al. "Epigenetics of Latent Epstein-Barr Virus Genomes: High Resolution Methylation Analysis of the Bidirectional Promoter Region of Latent Membrane Protein 1 and 2B Genes." Biological Chemistry, vol. 382, no. 4, 2001, pp. 699-705.
Takacs M, Salamon D, Myöhänen S, et al. Epigenetics of latent Epstein-Barr virus genomes: high resolution methylation analysis of the bidirectional promoter region of latent membrane protein 1 and 2B genes. Biol Chem. 2001;382(4):699-705.
Takacs, M., Salamon, D., Myöhänen, S., Li, H., Segesdi, J., Ujvari, D., Uhlig, J., Niller, H. H., Wolf, H., Berencsi, G., & Minarovits, J. (2001). Epigenetics of latent Epstein-Barr virus genomes: high resolution methylation analysis of the bidirectional promoter region of latent membrane protein 1 and 2B genes. Biological Chemistry, 382(4), 699-705.
Takacs M, et al. Epigenetics of Latent Epstein-Barr Virus Genomes: High Resolution Methylation Analysis of the Bidirectional Promoter Region of Latent Membrane Protein 1 and 2B Genes. Biol Chem. 2001;382(4):699-705. PubMed PMID: 11405234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epigenetics of latent Epstein-Barr virus genomes: high resolution methylation analysis of the bidirectional promoter region of latent membrane protein 1 and 2B genes. AU - Takacs,M, AU - Salamon,D, AU - Myöhänen,S, AU - Li,H, AU - Segesdi,J, AU - Ujvari,D, AU - Uhlig,J, AU - Niller,H H, AU - Wolf,H, AU - Berencsi,G, AU - Minarovits,J, PY - 2001/6/19/pubmed PY - 2002/1/5/medline PY - 2001/6/19/entrez SP - 699 EP - 705 JF - Biological chemistry JO - Biol. Chem. VL - 382 IS - 4 N2 - We analysed the methylation patterns of CpG dinucleotides in a bidirectional promoter region (LRS, LMP 1 regulatory sequences) of latent Epstein-Barr virus (EBV) genomes using automated fluorescent genomic sequencing after bisulfite-induced modification of DNA. Transcripts for two latent membrane proteins, LMP 1 (a transforming protein) and LMP 2B, are initiated in this region in opposite directions. We found that B cell lines and a clone expressing LMP 1 carried EBV genomes with unmethylated or hypomethylated LRS, while highly methylated CpG dinucleotides were present at each position or at discrete sites and within hypermethylated regions in LMP 1 negative cells. Comparison of high resolution methylation maps suggests that CpG methylation-mediated direct interference with binding of nuclear factors LBF 2, 3, 7, AML1/LBF1, LBF5 and LBF6 or methylation of CpGs within an E-box sequence (where activators as well as repressors can bind) is not the major mechanism in silencing of the LMP 1 promoter. Although a role for CpG methylation within binding sites of Sp1 and 3, ATF/CRE and a sis-inducible factor (SIF) cannot be excluded, hypermethylation of LRS or regions within LRS in LMP 1 negative cells suggests a role for an indirect mechanism, via methylcytosine binding proteins, in silencing of the LMP 1 promoter. SN - 1431-6730 UR - https://www.unboundmedicine.com/medline/citation/11405234/Epigenetics_of_latent_Epstein_Barr_virus_genomes:_high_resolution_methylation_analysis_of_the_bidirectional_promoter_region_of_latent_membrane_protein_1_and_2B_genes_ L2 - https://www.degruyter.com/doi/10.1515/BC.2001.083 DB - PRIME DP - Unbound Medicine ER -