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The u-boot mutation identifies a Hedgehog-regulated myogenic switch for fiber-type diversification in the zebrafish embryo.
Genes Dev. 2001 Jun 15; 15(12):1563-76.GD

Abstract

Developmental programs that govern the embryonic diversification of distinct kinds of muscles in vertebrates remain obscure. For instance, the most widely recognized attribute of early diversity among skeletal myoblasts is their ability to differentiate exclusively into fibers with slow or fast contractile properties. However, we know little about the developmental basis and genetic regulation of this seminal event in vertebrate myogenesis. Here we show that in the zebrafish, the u-boot gene acts as a myogenic switch that regulates the choice of myoblasts to adopt slow versus fast fiber developmental pathways. In u-boot mutant embryos, slow muscle precursors abort their developmental program, failing to activate expression of the homeobox gene prox1 and transfating into muscle cells with fast fiber properties. Using oligonucleotide-mediated translational inhibition, we have investigated the role of prox1 in this program. We find that it functions in the terminal step of the u-boot controlled slow fiber developmental pathway in the regulation of slow myofibril assembly. Our findings provide new insight into the genetic control of slow versus fast fiber specification and differentiation and indicate that dedicated developmental pathways exist in vertebrates for the elaboration of distinct elements of embryonic muscle pattern.

Authors+Show Affiliations

MRC Intercellular Signaling Group, Centre for Developmental Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11410536

Citation

Roy, S, et al. "The U-boot Mutation Identifies a Hedgehog-regulated Myogenic Switch for Fiber-type Diversification in the Zebrafish Embryo." Genes & Development, vol. 15, no. 12, 2001, pp. 1563-76.
Roy S, Wolff C, Ingham PW. The u-boot mutation identifies a Hedgehog-regulated myogenic switch for fiber-type diversification in the zebrafish embryo. Genes Dev. 2001;15(12):1563-76.
Roy, S., Wolff, C., & Ingham, P. W. (2001). The u-boot mutation identifies a Hedgehog-regulated myogenic switch for fiber-type diversification in the zebrafish embryo. Genes & Development, 15(12), 1563-76.
Roy S, Wolff C, Ingham PW. The U-boot Mutation Identifies a Hedgehog-regulated Myogenic Switch for Fiber-type Diversification in the Zebrafish Embryo. Genes Dev. 2001 Jun 15;15(12):1563-76. PubMed PMID: 11410536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The u-boot mutation identifies a Hedgehog-regulated myogenic switch for fiber-type diversification in the zebrafish embryo. AU - Roy,S, AU - Wolff,C, AU - Ingham,P W, PY - 2001/6/19/pubmed PY - 2001/7/28/medline PY - 2001/6/19/entrez SP - 1563 EP - 76 JF - Genes & development JO - Genes Dev VL - 15 IS - 12 N2 - Developmental programs that govern the embryonic diversification of distinct kinds of muscles in vertebrates remain obscure. For instance, the most widely recognized attribute of early diversity among skeletal myoblasts is their ability to differentiate exclusively into fibers with slow or fast contractile properties. However, we know little about the developmental basis and genetic regulation of this seminal event in vertebrate myogenesis. Here we show that in the zebrafish, the u-boot gene acts as a myogenic switch that regulates the choice of myoblasts to adopt slow versus fast fiber developmental pathways. In u-boot mutant embryos, slow muscle precursors abort their developmental program, failing to activate expression of the homeobox gene prox1 and transfating into muscle cells with fast fiber properties. Using oligonucleotide-mediated translational inhibition, we have investigated the role of prox1 in this program. We find that it functions in the terminal step of the u-boot controlled slow fiber developmental pathway in the regulation of slow myofibril assembly. Our findings provide new insight into the genetic control of slow versus fast fiber specification and differentiation and indicate that dedicated developmental pathways exist in vertebrates for the elaboration of distinct elements of embryonic muscle pattern. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/11410536/The_u_boot_mutation_identifies_a_Hedgehog_regulated_myogenic_switch_for_fiber_type_diversification_in_the_zebrafish_embryo_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=11410536 DB - PRIME DP - Unbound Medicine ER -