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Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner.
EMBO Rep 2001; 2(6):519-23ER

Abstract

The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co-reconstituted with the SecYEG complex. YidC had no effect on the SecA/SecYEG-mediated translocation of the secretory protein proOmpA; however, using a crosslinking approach, the transmembrane segment of nascent FtsQ was found to gain access to YidC via SecY. These data indicate the functional reconstitution of the initial stages of YidC-dependent membrane protein insertion via the SecYEG complex.

Authors+Show Affiliations

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11415985

Citation

van der Laan, M, et al. "Reconstitution of Sec-dependent Membrane Protein Insertion: Nascent FtsQ Interacts With YidC in a SecYEG-dependent Manner." EMBO Reports, vol. 2, no. 6, 2001, pp. 519-23.
van der Laan M, Houben EN, Nouwen N, et al. Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner. EMBO Rep. 2001;2(6):519-23.
van der Laan, M., Houben, E. N., Nouwen, N., Luirink, J., & Driessen, A. J. (2001). Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner. EMBO Reports, 2(6), pp. 519-23.
van der Laan M, et al. Reconstitution of Sec-dependent Membrane Protein Insertion: Nascent FtsQ Interacts With YidC in a SecYEG-dependent Manner. EMBO Rep. 2001;2(6):519-23. PubMed PMID: 11415985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner. AU - van der Laan,M, AU - Houben,E N, AU - Nouwen,N, AU - Luirink,J, AU - Driessen,A J, PY - 2001/6/21/pubmed PY - 2001/9/14/medline PY - 2001/6/21/entrez SP - 519 EP - 23 JF - EMBO reports JO - EMBO Rep. VL - 2 IS - 6 N2 - The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co-reconstituted with the SecYEG complex. YidC had no effect on the SecA/SecYEG-mediated translocation of the secretory protein proOmpA; however, using a crosslinking approach, the transmembrane segment of nascent FtsQ was found to gain access to YidC via SecY. These data indicate the functional reconstitution of the initial stages of YidC-dependent membrane protein insertion via the SecYEG complex. SN - 1469-221X UR - https://www.unboundmedicine.com/medline/citation/11415985/Reconstitution_of_Sec_dependent_membrane_protein_insertion:_nascent_FtsQ_interacts_with_YidC_in_a_SecYEG_dependent_manner_ L2 - http://embor.embopress.org/cgi/pmidlookup?view=long&pmid=11415985 DB - PRIME DP - Unbound Medicine ER -