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Quantitative analysis of hTERT mRNA expression in colorectal cancer.
Am J Gastroenterol. 2001 Jun; 96(6):1895-900.AJ

Abstract

OBJECTIVES

Telomerase is highly activated in a variety of malignant neoplasms including colon cancer. Among the major components of telomerase, human telomerase reverse transcriptase (hTERT) is thought to regulate telomerase activity. To assess the importance of telomerase for the diagnosis of colorectal cancer, we measured the expression of hTERT mRNA and telomerase activity in a large series of 140 colorectal cancers, 140 adjacent normal tissues, and 20 adenomas.

METHODS

The expression level of hTERT was measured quantitatively by competitive reverse transcriptase-polymerase chain reaction (RT-PCR), and telomerase activity was examined by telomeric repeat amplification protocol (TRAP) assay in the same samples.

RESULTS

The median expression level of hTERT mRNA in carcinomas was significantly higher than that in either adenomas or normal tissues. The median level of hTERT in adenomas was significantly higher than that in normal tissues. Telomerase activities in carcinomas were significantly higher than those in either adenomas or normal tissues. Telomerase activities in adenomas were also significantly higher than those in normal tissues. Furthermore, the relative expression levels of hTERT mRNA in adenomas and carcinomas were significantly correlated with the relative telomerase activities; the Spearman rank correlation was 0.53 (p = 0.021) and 0.18 (p = 0.031), respectively.

CONCLUSIONS

Our data suggest that determination of hTERT mRNA by competitive RT-PCR is superior in quantitative accuracy and sensitivity and would support the importance of telomerase activity for the diagnosis of colorectal cancer.

Authors+Show Affiliations

Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

11419845

Citation

Niiyama, H, et al. "Quantitative Analysis of hTERT mRNA Expression in Colorectal Cancer." The American Journal of Gastroenterology, vol. 96, no. 6, 2001, pp. 1895-900.
Niiyama H, Mizumoto K, Sato N, et al. Quantitative analysis of hTERT mRNA expression in colorectal cancer. Am J Gastroenterol. 2001;96(6):1895-900.
Niiyama, H., Mizumoto, K., Sato, N., Nagai, E., Mibu, R., Fukui, T., Kinoshita, M., & Tanaka, M. (2001). Quantitative analysis of hTERT mRNA expression in colorectal cancer. The American Journal of Gastroenterology, 96(6), 1895-900.
Niiyama H, et al. Quantitative Analysis of hTERT mRNA Expression in Colorectal Cancer. Am J Gastroenterol. 2001;96(6):1895-900. PubMed PMID: 11419845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of hTERT mRNA expression in colorectal cancer. AU - Niiyama,H, AU - Mizumoto,K, AU - Sato,N, AU - Nagai,E, AU - Mibu,R, AU - Fukui,T, AU - Kinoshita,M, AU - Tanaka,M, PY - 2001/6/23/pubmed PY - 2001/8/31/medline PY - 2001/6/23/entrez SP - 1895 EP - 900 JF - The American journal of gastroenterology JO - Am J Gastroenterol VL - 96 IS - 6 N2 - OBJECTIVES: Telomerase is highly activated in a variety of malignant neoplasms including colon cancer. Among the major components of telomerase, human telomerase reverse transcriptase (hTERT) is thought to regulate telomerase activity. To assess the importance of telomerase for the diagnosis of colorectal cancer, we measured the expression of hTERT mRNA and telomerase activity in a large series of 140 colorectal cancers, 140 adjacent normal tissues, and 20 adenomas. METHODS: The expression level of hTERT was measured quantitatively by competitive reverse transcriptase-polymerase chain reaction (RT-PCR), and telomerase activity was examined by telomeric repeat amplification protocol (TRAP) assay in the same samples. RESULTS: The median expression level of hTERT mRNA in carcinomas was significantly higher than that in either adenomas or normal tissues. The median level of hTERT in adenomas was significantly higher than that in normal tissues. Telomerase activities in carcinomas were significantly higher than those in either adenomas or normal tissues. Telomerase activities in adenomas were also significantly higher than those in normal tissues. Furthermore, the relative expression levels of hTERT mRNA in adenomas and carcinomas were significantly correlated with the relative telomerase activities; the Spearman rank correlation was 0.53 (p = 0.021) and 0.18 (p = 0.031), respectively. CONCLUSIONS: Our data suggest that determination of hTERT mRNA by competitive RT-PCR is superior in quantitative accuracy and sensitivity and would support the importance of telomerase activity for the diagnosis of colorectal cancer. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/11419845/Quantitative_analysis_of_hTERT_mRNA_expression_in_colorectal_cancer_ L2 - https://Insights.ovid.com/pubmed?pmid=11419845 DB - PRIME DP - Unbound Medicine ER -