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Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats.
J Biochem Mol Toxicol. 2001; 15(3):143-9.JB

Abstract

In light of evidence that some complications of diabetes mellitus may be caused or exacerbated by oxidative damage, we investigated the effects of subacute treatment with the antioxidant quercetin on tissue antioxidant defense systems in streptozotocin-induced diabetic Sprague-Dawley rats (30 days after streptozotocin induction). Quercetin, 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one, was administered at a dose of 10mg/kg/day, ip for 14 days, after which liver, kidney, brain, and heart were assayed for degree of lipid peroxidation, reduced and oxidized glutathione content, and activities of the free-radical detoxifying enzymes catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase. Treatment of normal rats with quercetin increased serum AST and increased hepatic concentration of oxidized glutathione. All tissues from diabetic animals exhibited disturbances in antioxidant defense when compared with normal controls. Quercetin treatment of diabetic rats reversed only the diabetic effects on brain oxidized glutathione concentration and on hepatic glutathione peroxidase activity. By contrast, a 20% increase in hepatic lipid peroxidation, a 40% decline in hepatic glutathione concentration, an increase in renal (23%) and cardiac (40%) glutathione peroxidase activities, and a 65% increase in cardiac catalase activity reflect intensified diabetic effects after treatment with quercetin. These results call into question the ability of therapy with the antioxidant quercetin to reverse diabetic oxidative stress in an overall sense.

Authors+Show Affiliations

Medical Sciences Program, Indiana University School of Medicine, Bloomington, IN 47405-7005, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11424224

Citation

Sanders, R A., et al. "Effects of Quercetin On Antioxidant Defense in Streptozotocin-induced Diabetic Rats." Journal of Biochemical and Molecular Toxicology, vol. 15, no. 3, 2001, pp. 143-9.
Sanders RA, Rauscher FM, Watkins JB. Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. J Biochem Mol Toxicol. 2001;15(3):143-9.
Sanders, R. A., Rauscher, F. M., & Watkins, J. B. (2001). Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. Journal of Biochemical and Molecular Toxicology, 15(3), 143-9.
Sanders RA, Rauscher FM, Watkins JB. Effects of Quercetin On Antioxidant Defense in Streptozotocin-induced Diabetic Rats. J Biochem Mol Toxicol. 2001;15(3):143-9. PubMed PMID: 11424224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. AU - Sanders,R A, AU - Rauscher,F M, AU - Watkins,J B,3rd PY - 2001/6/26/pubmed PY - 2001/9/28/medline PY - 2001/6/26/entrez SP - 143 EP - 9 JF - Journal of biochemical and molecular toxicology JO - J Biochem Mol Toxicol VL - 15 IS - 3 N2 - In light of evidence that some complications of diabetes mellitus may be caused or exacerbated by oxidative damage, we investigated the effects of subacute treatment with the antioxidant quercetin on tissue antioxidant defense systems in streptozotocin-induced diabetic Sprague-Dawley rats (30 days after streptozotocin induction). Quercetin, 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one, was administered at a dose of 10mg/kg/day, ip for 14 days, after which liver, kidney, brain, and heart were assayed for degree of lipid peroxidation, reduced and oxidized glutathione content, and activities of the free-radical detoxifying enzymes catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase. Treatment of normal rats with quercetin increased serum AST and increased hepatic concentration of oxidized glutathione. All tissues from diabetic animals exhibited disturbances in antioxidant defense when compared with normal controls. Quercetin treatment of diabetic rats reversed only the diabetic effects on brain oxidized glutathione concentration and on hepatic glutathione peroxidase activity. By contrast, a 20% increase in hepatic lipid peroxidation, a 40% decline in hepatic glutathione concentration, an increase in renal (23%) and cardiac (40%) glutathione peroxidase activities, and a 65% increase in cardiac catalase activity reflect intensified diabetic effects after treatment with quercetin. These results call into question the ability of therapy with the antioxidant quercetin to reverse diabetic oxidative stress in an overall sense. SN - 1095-6670 UR - https://www.unboundmedicine.com/medline/citation/11424224/Effects_of_quercetin_on_antioxidant_defense_in_streptozotocin_induced_diabetic_rats_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1095-6670&date=2001&volume=15&issue=3&spage=143 DB - PRIME DP - Unbound Medicine ER -