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Effects of isoeugenol on oxidative stress pathways in normal and streptozotocin-induced diabetic rats.
J Biochem Mol Toxicol. 2001; 15(3):159-64.JB

Abstract

Because some complications of diabetes mellitus may result from oxidative damage, we investigated the effects of subacute treatment (10mg/kg/day, intraperitoneal [ip], for 14 days) with the antioxidant isoeugenol on the oxidant defense system in normal and 30-day streptozotocin-induced diabetic Sprague-Dawley rats. Liver, kidney, brain, and heart were assayed for degree of lipid peroxidation, reduced and oxidized glutathione content, and activities of the free radical-detoxifying enzymes catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase. All tissues from diabetic animals exhibited disturbances in antioxidant defense when compared with normal controls. Treatment with isoeugenol reversed diabetic effects on hepatic glutathione peroxidase activity and on oxidized glutathione concentration in brain. Treatment with the lipophilic compound isoeugenol also decreased lipid peroxidation in both liver and heart of normal animals and decreased hepatic oxidized glutathione content in both normal and diabetic rats. Some effects of isoeugenol treatment, such as decreased activity of hepatic superoxide dismutase and glutathione reductase in diabetic rats, were unrelated to the oxidative effects of diabetes. In heart of diabetic animals, isoeugenol treatment resulted in an exacerbation of already elevated activities of catalase. These results indicate that isoeugenol therapy may not reverse diabetic oxidative stress in an overall sense.

Authors+Show Affiliations

Medical Sciences Program, Indiana University School of Medicine, Bloomington, IN 47405-7005, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11424226

Citation

Rauscher, F M., et al. "Effects of Isoeugenol On Oxidative Stress Pathways in Normal and Streptozotocin-induced Diabetic Rats." Journal of Biochemical and Molecular Toxicology, vol. 15, no. 3, 2001, pp. 159-64.
Rauscher FM, Sanders RA, Watkins JB. Effects of isoeugenol on oxidative stress pathways in normal and streptozotocin-induced diabetic rats. J Biochem Mol Toxicol. 2001;15(3):159-64.
Rauscher, F. M., Sanders, R. A., & Watkins, J. B. (2001). Effects of isoeugenol on oxidative stress pathways in normal and streptozotocin-induced diabetic rats. Journal of Biochemical and Molecular Toxicology, 15(3), 159-64.
Rauscher FM, Sanders RA, Watkins JB. Effects of Isoeugenol On Oxidative Stress Pathways in Normal and Streptozotocin-induced Diabetic Rats. J Biochem Mol Toxicol. 2001;15(3):159-64. PubMed PMID: 11424226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of isoeugenol on oxidative stress pathways in normal and streptozotocin-induced diabetic rats. AU - Rauscher,F M, AU - Sanders,R A, AU - Watkins,J B,3rd PY - 2001/6/26/pubmed PY - 2001/9/28/medline PY - 2001/6/26/entrez SP - 159 EP - 64 JF - Journal of biochemical and molecular toxicology JO - J Biochem Mol Toxicol VL - 15 IS - 3 N2 - Because some complications of diabetes mellitus may result from oxidative damage, we investigated the effects of subacute treatment (10mg/kg/day, intraperitoneal [ip], for 14 days) with the antioxidant isoeugenol on the oxidant defense system in normal and 30-day streptozotocin-induced diabetic Sprague-Dawley rats. Liver, kidney, brain, and heart were assayed for degree of lipid peroxidation, reduced and oxidized glutathione content, and activities of the free radical-detoxifying enzymes catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase. All tissues from diabetic animals exhibited disturbances in antioxidant defense when compared with normal controls. Treatment with isoeugenol reversed diabetic effects on hepatic glutathione peroxidase activity and on oxidized glutathione concentration in brain. Treatment with the lipophilic compound isoeugenol also decreased lipid peroxidation in both liver and heart of normal animals and decreased hepatic oxidized glutathione content in both normal and diabetic rats. Some effects of isoeugenol treatment, such as decreased activity of hepatic superoxide dismutase and glutathione reductase in diabetic rats, were unrelated to the oxidative effects of diabetes. In heart of diabetic animals, isoeugenol treatment resulted in an exacerbation of already elevated activities of catalase. These results indicate that isoeugenol therapy may not reverse diabetic oxidative stress in an overall sense. SN - 1095-6670 UR - https://www.unboundmedicine.com/medline/citation/11424226/Effects_of_isoeugenol_on_oxidative_stress_pathways_in_normal_and_streptozotocin_induced_diabetic_rats_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1095-6670&date=2001&volume=15&issue=3&spage=159 DB - PRIME DP - Unbound Medicine ER -