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Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery.
Eur J Pharmacol. 2001 May 11; 419(2-3):231-42.EJ

Abstract

Mechanisms underlying acetylcholine-induced endothelium-independent vasodilation were studied in the rat mesenteric vascular bed isolated from Wistar rats. In preparations without endothelium, and contracted by perfusion with Krebs solution containing methoxamine (2-7 microM), perfusion of acetylcholine (1-100 microM) for 1 min produced a concentration-dependent vasodilation. Denervation of denuded preparations by cold storage (4 degrees C for 72 h) abolished the acetylcholine-induced vasodilation; 10 and 100 nM atropine abolished 1 and 10 microM acetylcholine-induced vasodilation, but it inhibited only 20% of vasodilation by 100 microM acetylcholine. The acetylcholine-induced atropine-resistant vasodilation was inhibited by 10 and 100 microM hexamethonium, 5 microM guanethidine, 50 microM bretylium, in vitro 6-hydroxydopamine (2 mM for 20 min, twice), 1 microM capsaicin and 0.5 microM calcitonin gene-related peptide (CGRP)-(8-37) (CGRP receptor antagonist). These findings suggest that the acetylcholine-induced endothelium-independent nicotinic vasodilation requires the presence of intact adrenergic nerves, and is mediated by endogenous CGRP released from CGRP-containing nerves.

Authors+Show Affiliations

Department of Clinical Pharmaceutical Science, Graduate School of Natural Science and Technology, Okayama University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11426846

Citation

Shiraki, H, et al. "Adrenergic Nerves Mediate Acetylcholine-induced Endothelium-independent Vasodilation in the Rat Mesenteric Resistance Artery." European Journal of Pharmacology, vol. 419, no. 2-3, 2001, pp. 231-42.
Shiraki H, Kawasaki H, Tezuka S, et al. Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery. Eur J Pharmacol. 2001;419(2-3):231-42.
Shiraki, H., Kawasaki, H., Tezuka, S., Nakatsuma, A., Nawa, H., Araki, H., Gomita, Y., & Kurosaki, Y. (2001). Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery. European Journal of Pharmacology, 419(2-3), 231-42.
Shiraki H, et al. Adrenergic Nerves Mediate Acetylcholine-induced Endothelium-independent Vasodilation in the Rat Mesenteric Resistance Artery. Eur J Pharmacol. 2001 May 11;419(2-3):231-42. PubMed PMID: 11426846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery. AU - Shiraki,H, AU - Kawasaki,H, AU - Tezuka,S, AU - Nakatsuma,A, AU - Nawa,H, AU - Araki,H, AU - Gomita,Y, AU - Kurosaki,Y, PY - 2001/6/28/pubmed PY - 2001/11/3/medline PY - 2001/6/28/entrez SP - 231 EP - 42 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 419 IS - 2-3 N2 - Mechanisms underlying acetylcholine-induced endothelium-independent vasodilation were studied in the rat mesenteric vascular bed isolated from Wistar rats. In preparations without endothelium, and contracted by perfusion with Krebs solution containing methoxamine (2-7 microM), perfusion of acetylcholine (1-100 microM) for 1 min produced a concentration-dependent vasodilation. Denervation of denuded preparations by cold storage (4 degrees C for 72 h) abolished the acetylcholine-induced vasodilation; 10 and 100 nM atropine abolished 1 and 10 microM acetylcholine-induced vasodilation, but it inhibited only 20% of vasodilation by 100 microM acetylcholine. The acetylcholine-induced atropine-resistant vasodilation was inhibited by 10 and 100 microM hexamethonium, 5 microM guanethidine, 50 microM bretylium, in vitro 6-hydroxydopamine (2 mM for 20 min, twice), 1 microM capsaicin and 0.5 microM calcitonin gene-related peptide (CGRP)-(8-37) (CGRP receptor antagonist). These findings suggest that the acetylcholine-induced endothelium-independent nicotinic vasodilation requires the presence of intact adrenergic nerves, and is mediated by endogenous CGRP released from CGRP-containing nerves. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/11426846/Adrenergic_nerves_mediate_acetylcholine_induced_endothelium_independent_vasodilation_in_the_rat_mesenteric_resistance_artery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(01)00981-5 DB - PRIME DP - Unbound Medicine ER -