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Comparison of cell-wall polysaccharides from Nectria cinnabarina with those from the group of Nectria with Sesquicillium anamorphs.
Microbiology (Reading). 2001 Jul; 147(Pt 7):1839-1849.M

Abstract

Alkali-extractable and water-soluble polysaccharides were purified from cell walls of five species of Sesquicillium or its teleomorphs, Nectria lasiacidis and Nectria impariphialis, and from Nectria cinnabarina, the type species of Nectria, a heterogeneous genus that belongs to the Hypocreales. Methylation and NMR analyses for determination of linkage types and structure were performed and indicated differences between the polysaccharides purified during the present study and those isolated from other nectrioid fungi, namely the presence of 5-O-substituted galactofuranose (-->5)-Galf-(1-->) in the main chain together with 2,6-di-O-substituted galactofuranose (-->2,6)-Galf-(1-->) residues in Sesquicillium buxi and Sesquicillium pseudosetosum. The polysaccharide from N. impariphialis was similar to those obtained from the above species, although an additional residue of 6-O-substituted glucopyranose (-->6)-Glcp-(1-->), was detected in some side chains. In N. lasiacidis and Sesquicillium candelabrum the polysaccharide contained an additional branching point of 5,6-di-O-substituted galactofuranose (-->5,6)-Galf-(1-->) linked to terminal N-acetylglucosamine GlcNAc-(1-->). These chains were linked to a small mannan core. All these polysaccharides showed major differences to the polysaccharide of N. cinnabarina, which was formed by a main chain of (1-->6)-beta-linked galactofuranose units almost fully branched at positions 2-O by either single residues of glucopyranose or acidic chains containing glucuronic acid and mannose.

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Authors+Show Affiliations

Ahrazem O
Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain1.
Prieto A
Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain1.
Gómez-Miranda B
Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain1.
Bernabé M
Instituto de Quı́mica Orgánica, Departamento de Quı́mica Orgánica Biológica, CSIC, Juan de la Cierva 3, 28006-Madrid, Spain2.
Leal JA
Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain1.

MeSH

AscomycotaCarbohydrate ConformationCarbohydrate SequenceCell WallMagnetic Resonance SpectroscopyMolecular Sequence DataPolysaccharides

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11429461

Citation

Ahrazem, Oussama, et al. "Comparison of Cell-wall Polysaccharides From Nectria Cinnabarina With Those From the Group of Nectria With Sesquicillium Anamorphs." Microbiology (Reading, England), vol. 147, no. Pt 7, 2001, pp. 1839-1849.
Ahrazem O, Prieto A, Gómez-Miranda B, et al. Comparison of cell-wall polysaccharides from Nectria cinnabarina with those from the group of Nectria with Sesquicillium anamorphs. Microbiology (Reading). 2001;147(Pt 7):1839-1849.
Ahrazem, O., Prieto, A., Gómez-Miranda, B., Bernabé, M., & Leal, J. A. (2001). Comparison of cell-wall polysaccharides from Nectria cinnabarina with those from the group of Nectria with Sesquicillium anamorphs. Microbiology (Reading, England), 147(Pt 7), 1839-1849. https://doi.org/10.1099/00221287-147-7-1839
Ahrazem O, et al. Comparison of Cell-wall Polysaccharides From Nectria Cinnabarina With Those From the Group of Nectria With Sesquicillium Anamorphs. Microbiology (Reading). 2001;147(Pt 7):1839-1849. PubMed PMID: 11429461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of cell-wall polysaccharides from Nectria cinnabarina with those from the group of Nectria with Sesquicillium anamorphs. AU - Ahrazem,Oussama, AU - Prieto,Alicia, AU - Gómez-Miranda,Begoña, AU - Bernabé,Manuel, AU - Leal,J Antonio, PY - 2001/6/29/pubmed PY - 2002/1/5/medline PY - 2001/6/29/entrez SP - 1839 EP - 1849 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 147 IS - Pt 7 N2 - Alkali-extractable and water-soluble polysaccharides were purified from cell walls of five species of Sesquicillium or its teleomorphs, Nectria lasiacidis and Nectria impariphialis, and from Nectria cinnabarina, the type species of Nectria, a heterogeneous genus that belongs to the Hypocreales. Methylation and NMR analyses for determination of linkage types and structure were performed and indicated differences between the polysaccharides purified during the present study and those isolated from other nectrioid fungi, namely the presence of 5-O-substituted galactofuranose (-->5)-Galf-(1-->) in the main chain together with 2,6-di-O-substituted galactofuranose (-->2,6)-Galf-(1-->) residues in Sesquicillium buxi and Sesquicillium pseudosetosum. The polysaccharide from N. impariphialis was similar to those obtained from the above species, although an additional residue of 6-O-substituted glucopyranose (-->6)-Glcp-(1-->), was detected in some side chains. In N. lasiacidis and Sesquicillium candelabrum the polysaccharide contained an additional branching point of 5,6-di-O-substituted galactofuranose (-->5,6)-Galf-(1-->) linked to terminal N-acetylglucosamine GlcNAc-(1-->). These chains were linked to a small mannan core. All these polysaccharides showed major differences to the polysaccharide of N. cinnabarina, which was formed by a main chain of (1-->6)-beta-linked galactofuranose units almost fully branched at positions 2-O by either single residues of glucopyranose or acidic chains containing glucuronic acid and mannose. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/11429461/Comparison_of_cell_wall_polysaccharides_from_Nectria_cinnabarina_with_those_from_the_group_of_Nectria_with_Sesquicillium_anamorphs_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/00221287-147-7-1839 DB - PRIME DP - Unbound Medicine ER -