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The effect of TGF-beta1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array.
Invest Ophthalmol Vis Sci. 2001 Jul; 42(8):1691-7.IO

Abstract

PURPOSE

TGF-betas regulate cell proliferation and differentiation, and they play important roles in maintenance of corneal epithelium. However, the precise function of TGF-betas in the corneal epithelium remains unclear. In this study, cDNA expression array technology was used to demonstrate the effect of TGF-beta1 on the simultaneous expression of a large number of genes in cultured human corneal epithelial cells (HCECs). The change in protein level expression of the specific genes influenced by TGF-beta1 was also investigated.

METHODS

Human cDNA expression array technology was used to study the simultaneous expression of 1176 specific cellular genes in HCECs incubated with TGF-beta1 (10 ng/ml). Moreover, gene-specific semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to confirm the gene expression pattern measured by the cDNA expression array. Western blot analysis was used to examine protein expression of the specific genes in the presence or absence of TGF-beta1.

RESULTS

TGF-beta1 significantly upregulated the expression of 19 genes and significantly downregulated ras-related protein, caspase10, and beta4-integrin in the treated HCECs. The expression of 277 genes including alpha3-integrin, PAI-2, transferrin receptor, and cyclin-D1 was studied. Semiquantitative RT-PCR analysis confirmed the TGF-beta1-mediated changes in expression patterns of these genes. Furthermore, Western blot analysis revealed that TGF-beta1 remarkably decreased PAI-2, transferrin receptor, and integrin alpha3, and increased caspase10 on the protein level.

CONCLUSIONS

TGF-beta1 regulates the expression of specific types of genes in HCECs. These results strongly suggest that TGF-beta1 is critically involved in the maintenance of the corneal epithelium through the control of a network of various signal-transduction pathways.

Authors+Show Affiliations

Department of Ophthalmology, Osaka University Medical School, E-7, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11431430

Citation

Hayashida-Hibino, S, et al. "The Effect of TGF-beta1 On Differential Gene Expression Profiles in Human Corneal Epithelium Studied By cDNA Expression Array." Investigative Ophthalmology & Visual Science, vol. 42, no. 8, 2001, pp. 1691-7.
Hayashida-Hibino S, Watanabe H, Nishida K, et al. The effect of TGF-beta1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array. Invest Ophthalmol Vis Sci. 2001;42(8):1691-7.
Hayashida-Hibino, S., Watanabe, H., Nishida, K., Tsujikawa, M., Tanaka, T., Hori, Y., Saishin, Y., & Tano, Y. (2001). The effect of TGF-beta1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array. Investigative Ophthalmology & Visual Science, 42(8), 1691-7.
Hayashida-Hibino S, et al. The Effect of TGF-beta1 On Differential Gene Expression Profiles in Human Corneal Epithelium Studied By cDNA Expression Array. Invest Ophthalmol Vis Sci. 2001;42(8):1691-7. PubMed PMID: 11431430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of TGF-beta1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array. AU - Hayashida-Hibino,S, AU - Watanabe,H, AU - Nishida,K, AU - Tsujikawa,M, AU - Tanaka,T, AU - Hori,Y, AU - Saishin,Y, AU - Tano,Y, PY - 2001/6/30/pubmed PY - 2001/8/3/medline PY - 2001/6/30/entrez SP - 1691 EP - 7 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 42 IS - 8 N2 - PURPOSE: TGF-betas regulate cell proliferation and differentiation, and they play important roles in maintenance of corneal epithelium. However, the precise function of TGF-betas in the corneal epithelium remains unclear. In this study, cDNA expression array technology was used to demonstrate the effect of TGF-beta1 on the simultaneous expression of a large number of genes in cultured human corneal epithelial cells (HCECs). The change in protein level expression of the specific genes influenced by TGF-beta1 was also investigated. METHODS: Human cDNA expression array technology was used to study the simultaneous expression of 1176 specific cellular genes in HCECs incubated with TGF-beta1 (10 ng/ml). Moreover, gene-specific semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to confirm the gene expression pattern measured by the cDNA expression array. Western blot analysis was used to examine protein expression of the specific genes in the presence or absence of TGF-beta1. RESULTS: TGF-beta1 significantly upregulated the expression of 19 genes and significantly downregulated ras-related protein, caspase10, and beta4-integrin in the treated HCECs. The expression of 277 genes including alpha3-integrin, PAI-2, transferrin receptor, and cyclin-D1 was studied. Semiquantitative RT-PCR analysis confirmed the TGF-beta1-mediated changes in expression patterns of these genes. Furthermore, Western blot analysis revealed that TGF-beta1 remarkably decreased PAI-2, transferrin receptor, and integrin alpha3, and increased caspase10 on the protein level. CONCLUSIONS: TGF-beta1 regulates the expression of specific types of genes in HCECs. These results strongly suggest that TGF-beta1 is critically involved in the maintenance of the corneal epithelium through the control of a network of various signal-transduction pathways. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/11431430/The_effect_of_TGF_beta1_on_differential_gene_expression_profiles_in_human_corneal_epithelium_studied_by_cDNA_expression_array_ L2 - https://iovs.arvojournals.org/article.aspx?volume=42&issue=8&page=1691 DB - PRIME DP - Unbound Medicine ER -