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Homozygous deletion in a neuroblastoma cell line defined by a high-density STS map spanning human chromosome band 1p36.
Genes Chromosomes Cancer. 2001 Aug; 31(4):326-32.GC

Abstract

Recent molecular studies have shown a relatively high rate of loss of heterozygosity (LOH) in neuroblastoma (NB) as well as other types of tumors in human chromosome band 1p36. To identify candidate tumor suppressor genes in NB, we searched for homozygous deletions in NB cell lines with PCR according to a high-density sequence tagged site (STS)-content map spanning 1p35-36. Among 25 NB cell lines examined, only one cell line, NB-1, showed no signal with 27 STSs in a 480 kb region in 1p36.2. The sequence analysis has revealed that the defective region included seven known genes (E4, KIF1B, SCYA5, PGD, Cortistatin, DFF45, and PEX14), nine expressed sequence tags (ESTs), and two microsatellite markers. These genes are related to apoptosis, an ubiquitin-proteasome pathway, a neuronal microtubule-associated motor molecule, and components of a common translocation machinery. The region between the DFF45 and KIF1B genes was defined as homozygous deletion by Southern blotting. The search in LOH regions with high-density STSs may be useful for the isolation and identification of tumor suppressor genes in other tumors as well as NBs.

Authors+Show Affiliations

Gene Bank, Tsukuba Institute, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11433523

Citation

Chen, Y Z., et al. "Homozygous Deletion in a Neuroblastoma Cell Line Defined By a High-density STS Map Spanning Human Chromosome Band 1p36." Genes, Chromosomes & Cancer, vol. 31, no. 4, 2001, pp. 326-32.
Chen YZ, Soeda E, Yang HW, et al. Homozygous deletion in a neuroblastoma cell line defined by a high-density STS map spanning human chromosome band 1p36. Genes Chromosomes Cancer. 2001;31(4):326-32.
Chen, Y. Z., Soeda, E., Yang, H. W., Takita, J., Chai, L., Horii, A., Inazawa, J., Ohki, M., & Hayashi, Y. (2001). Homozygous deletion in a neuroblastoma cell line defined by a high-density STS map spanning human chromosome band 1p36. Genes, Chromosomes & Cancer, 31(4), 326-32.
Chen YZ, et al. Homozygous Deletion in a Neuroblastoma Cell Line Defined By a High-density STS Map Spanning Human Chromosome Band 1p36. Genes Chromosomes Cancer. 2001;31(4):326-32. PubMed PMID: 11433523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Homozygous deletion in a neuroblastoma cell line defined by a high-density STS map spanning human chromosome band 1p36. AU - Chen,Y Z, AU - Soeda,E, AU - Yang,H W, AU - Takita,J, AU - Chai,L, AU - Horii,A, AU - Inazawa,J, AU - Ohki,M, AU - Hayashi,Y, PY - 2001/7/4/pubmed PY - 2001/8/3/medline PY - 2001/7/4/entrez SP - 326 EP - 32 JF - Genes, chromosomes & cancer JO - Genes Chromosomes Cancer VL - 31 IS - 4 N2 - Recent molecular studies have shown a relatively high rate of loss of heterozygosity (LOH) in neuroblastoma (NB) as well as other types of tumors in human chromosome band 1p36. To identify candidate tumor suppressor genes in NB, we searched for homozygous deletions in NB cell lines with PCR according to a high-density sequence tagged site (STS)-content map spanning 1p35-36. Among 25 NB cell lines examined, only one cell line, NB-1, showed no signal with 27 STSs in a 480 kb region in 1p36.2. The sequence analysis has revealed that the defective region included seven known genes (E4, KIF1B, SCYA5, PGD, Cortistatin, DFF45, and PEX14), nine expressed sequence tags (ESTs), and two microsatellite markers. These genes are related to apoptosis, an ubiquitin-proteasome pathway, a neuronal microtubule-associated motor molecule, and components of a common translocation machinery. The region between the DFF45 and KIF1B genes was defined as homozygous deletion by Southern blotting. The search in LOH regions with high-density STSs may be useful for the isolation and identification of tumor suppressor genes in other tumors as well as NBs. SN - 1045-2257 UR - https://www.unboundmedicine.com/medline/citation/11433523/Homozygous_deletion_in_a_neuroblastoma_cell_line_defined_by_a_high_density_STS_map_spanning_human_chromosome_band_1p36_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1045-2257&date=2001&volume=31&issue=4&spage=326 DB - PRIME DP - Unbound Medicine ER -