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Determination of trimebutine maleate in rat plasma and tissues by using capillary zone electrophoresis.
Biomed Chromatogr. 2001 Jun; 15(4):248-51.BC

Abstract

A simple and rapid capillary zone electrophoresis method was developed for the determination of trimebutine maleate in rat plasma and tissues. Rat plasma and tissue homogenates were mixed with acetonitrile containing internal standard, ephedrine hydrochloride, and then centrifuged. The supernatant was dried under a stream of nitrogen, and the residue was reconstituted in methanol-water (1:1). The electrophoresis was performed in uncoated capillary with 30 mmol/L phosphate buffer of pH 6.0 as the separation electrolyte. The applied voltage was 10 kV and the UV detection was set at 214 nm. The peak height ratio vs concentration in plasma or homogenates was linear over the range of 5-500 ng/mL and the limit of quantitation was 5 ng/mL. The intra- and inter-day precision was RSD < 14% and <15%. The accuracy was relative error (RE) within +/- 14%. This method was applied to studying the pharmacokinetics and tissue distribution after a single dose of trimebutine maleate was administrated to the rats. The T(max), AUC, C(max) and t(1/2) were 30 min, 7.8 x 10(2) (ng/mL) min, 39 ng/mL and 1.7 x 10(2) min. The drug distribution was found in a decreasing order of liver, kidney, spleen, lung and heart.

Authors+Show Affiliations

Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang, People's Republic of China. fameili@ihw.com.cnNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11438965

Citation

Li, F, and L Yu. "Determination of Trimebutine Maleate in Rat Plasma and Tissues By Using Capillary Zone Electrophoresis." Biomedical Chromatography : BMC, vol. 15, no. 4, 2001, pp. 248-51.
Li F, Yu L. Determination of trimebutine maleate in rat plasma and tissues by using capillary zone electrophoresis. Biomed Chromatogr. 2001;15(4):248-51.
Li, F., & Yu, L. (2001). Determination of trimebutine maleate in rat plasma and tissues by using capillary zone electrophoresis. Biomedical Chromatography : BMC, 15(4), 248-51.
Li F, Yu L. Determination of Trimebutine Maleate in Rat Plasma and Tissues By Using Capillary Zone Electrophoresis. Biomed Chromatogr. 2001;15(4):248-51. PubMed PMID: 11438965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of trimebutine maleate in rat plasma and tissues by using capillary zone electrophoresis. AU - Li,F, AU - Yu,L, PY - 2001/7/5/pubmed PY - 2001/9/21/medline PY - 2001/7/5/entrez SP - 248 EP - 51 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 15 IS - 4 N2 - A simple and rapid capillary zone electrophoresis method was developed for the determination of trimebutine maleate in rat plasma and tissues. Rat plasma and tissue homogenates were mixed with acetonitrile containing internal standard, ephedrine hydrochloride, and then centrifuged. The supernatant was dried under a stream of nitrogen, and the residue was reconstituted in methanol-water (1:1). The electrophoresis was performed in uncoated capillary with 30 mmol/L phosphate buffer of pH 6.0 as the separation electrolyte. The applied voltage was 10 kV and the UV detection was set at 214 nm. The peak height ratio vs concentration in plasma or homogenates was linear over the range of 5-500 ng/mL and the limit of quantitation was 5 ng/mL. The intra- and inter-day precision was RSD < 14% and <15%. The accuracy was relative error (RE) within +/- 14%. This method was applied to studying the pharmacokinetics and tissue distribution after a single dose of trimebutine maleate was administrated to the rats. The T(max), AUC, C(max) and t(1/2) were 30 min, 7.8 x 10(2) (ng/mL) min, 39 ng/mL and 1.7 x 10(2) min. The drug distribution was found in a decreasing order of liver, kidney, spleen, lung and heart. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/11438965/Determination_of_trimebutine_maleate_in_rat_plasma_and_tissues_by_using_capillary_zone_electrophoresis_ L2 - https://doi.org/10.1002/bmc.52 DB - PRIME DP - Unbound Medicine ER -