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Comparison of the effects of dietary n-3 and n-6 polyunsaturated fatty acids on very-low-density lipoprotein secretion when delivered to hepatocytes in chylomicron remnants.
Biochem J. 2001 Jul 15; 357(Pt 2):481-7.BJ

Abstract

The effects of chylomicron remnants enriched in n-3 or n-6 polyunsaturated fatty acids (derived from fish or corn oil respectively) on the secretion of very-low-density lipoprotein (VLDL) lipid and apolipoprotein B (apoB) by rat hepatocytes in culture was investigated. Remnants were prepared in vivo from chylomicrons obtained from rats given an oral dose of fish or corn oil and incubated with cultured hepatocytes for up to 16 h. The medium was then removed and the secretion of cholesterol and triacylglycerol into the whole medium or the rho<1.050 g/ml fraction during the following 7-24 h was determined. After exposure of the cells to fish-oil as compared with corn-oil remnants, secretion of both cholesterol and triacylglycerol into the whole medium was decreased by 25-35%, and secretion into the rho<1.050 g/ml fraction was decreased by 20-25%. In addition, the levels of apoB48 found in the rho<1.050 g/ml fraction were significantly lower in cells treated with fish-oil rather than corn-oil remnants, although the levels of apoB100 remained unchanged. The expression of mRNA for apoB, as determined by reverse-transcriptase PCR, however, was not significantly changed after exposure of the cells to both types of remnants. These results demonstrate that the effects of dietary n-3 polyunsaturated fatty acids in depressing hepatic VLDL secretion occur directly when they are delivered to the liver from the intestine in chylomicron remnants, and that the secretion, but not the synthesis, of apoB is targeted.

Authors+Show Affiliations

Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St., London NW1 0TU, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11439098

Citation

Zheng, X, et al. "Comparison of the Effects of Dietary N-3 and N-6 Polyunsaturated Fatty Acids On Very-low-density Lipoprotein Secretion when Delivered to Hepatocytes in Chylomicron Remnants." The Biochemical Journal, vol. 357, no. Pt 2, 2001, pp. 481-7.
Zheng X, Avella M, Botham KM. Comparison of the effects of dietary n-3 and n-6 polyunsaturated fatty acids on very-low-density lipoprotein secretion when delivered to hepatocytes in chylomicron remnants. Biochem J. 2001;357(Pt 2):481-7.
Zheng, X., Avella, M., & Botham, K. M. (2001). Comparison of the effects of dietary n-3 and n-6 polyunsaturated fatty acids on very-low-density lipoprotein secretion when delivered to hepatocytes in chylomicron remnants. The Biochemical Journal, 357(Pt 2), 481-7.
Zheng X, Avella M, Botham KM. Comparison of the Effects of Dietary N-3 and N-6 Polyunsaturated Fatty Acids On Very-low-density Lipoprotein Secretion when Delivered to Hepatocytes in Chylomicron Remnants. Biochem J. 2001 Jul 15;357(Pt 2):481-7. PubMed PMID: 11439098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the effects of dietary n-3 and n-6 polyunsaturated fatty acids on very-low-density lipoprotein secretion when delivered to hepatocytes in chylomicron remnants. AU - Zheng,X, AU - Avella,M, AU - Botham,K M, PY - 2001/7/6/pubmed PY - 2001/8/24/medline PY - 2001/7/6/entrez SP - 481 EP - 7 JF - The Biochemical journal JO - Biochem. J. VL - 357 IS - Pt 2 N2 - The effects of chylomicron remnants enriched in n-3 or n-6 polyunsaturated fatty acids (derived from fish or corn oil respectively) on the secretion of very-low-density lipoprotein (VLDL) lipid and apolipoprotein B (apoB) by rat hepatocytes in culture was investigated. Remnants were prepared in vivo from chylomicrons obtained from rats given an oral dose of fish or corn oil and incubated with cultured hepatocytes for up to 16 h. The medium was then removed and the secretion of cholesterol and triacylglycerol into the whole medium or the rho<1.050 g/ml fraction during the following 7-24 h was determined. After exposure of the cells to fish-oil as compared with corn-oil remnants, secretion of both cholesterol and triacylglycerol into the whole medium was decreased by 25-35%, and secretion into the rho<1.050 g/ml fraction was decreased by 20-25%. In addition, the levels of apoB48 found in the rho<1.050 g/ml fraction were significantly lower in cells treated with fish-oil rather than corn-oil remnants, although the levels of apoB100 remained unchanged. The expression of mRNA for apoB, as determined by reverse-transcriptase PCR, however, was not significantly changed after exposure of the cells to both types of remnants. These results demonstrate that the effects of dietary n-3 polyunsaturated fatty acids in depressing hepatic VLDL secretion occur directly when they are delivered to the liver from the intestine in chylomicron remnants, and that the secretion, but not the synthesis, of apoB is targeted. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/11439098/Comparison_of_the_effects_of_dietary_n_3_and_n_6_polyunsaturated_fatty_acids_on_very_low_density_lipoprotein_secretion_when_delivered_to_hepatocytes_in_chylomicron_remnants_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/0264-6021:3570481 DB - PRIME DP - Unbound Medicine ER -