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Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection.
Analyst. 2001 Jun; 126(6):816-20.A

Abstract

A high-performance liquid chromatographic method with electrochemical detection was developed for the determination of twelve tea catechins including four major catechins: epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG) and epigallocatechin gallate (EGCG); four of their epimers at the C-2 position, C, GC, CG and GCG; and four methylated catechin derivatives, epigallocatechin-3-O-(3-O-methyl)gallate, gallocatechin-3-O-(3-O-methyl)gallate, epigallocatechin-3-O-(4-O-methyl)gallate and epicatechin-3-O-(3-O-methyl)gallate. These catechins were separated on an ODS C18 reversed-phase column by isocratic elution with 0.1 M NaH2PO4 buffer (pH 2.5)-acetonitrile (87:13) containing 0.1 mM EDTA.2Na. The detection limits (S/N = 3) of these catechins were approximately 10-40 pmol ml-1 at an applied voltage of 600 mV. Extracting these catechins from tea leaf powder with H2O-acetonitrile (1:1) at 30 degrees C for 40 min inhibited the epimerization at C-2 significantly from these epicatechins compared to extraction with hot water at 90 degrees C. This analytical method is sensitive to and appropriate for the simultaneous determination of various biologically active catechins in green tea.

Authors+Show Affiliations

School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. sano@ys2.u-shizuoka-ken.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11445943

Citation

Sano, M, et al. "Simultaneous Determination of Twelve Tea Catechins By High-performance Liquid Chromatography With Electrochemical Detection." The Analyst, vol. 126, no. 6, 2001, pp. 816-20.
Sano M, Tabata M, Suzuki M, et al. Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection. Analyst. 2001;126(6):816-20.
Sano, M., Tabata, M., Suzuki, M., Degawa, M., Miyase, T., & Maeda-Yamamoto, M. (2001). Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection. The Analyst, 126(6), 816-20.
Sano M, et al. Simultaneous Determination of Twelve Tea Catechins By High-performance Liquid Chromatography With Electrochemical Detection. Analyst. 2001;126(6):816-20. PubMed PMID: 11445943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection. AU - Sano,M, AU - Tabata,M, AU - Suzuki,M, AU - Degawa,M, AU - Miyase,T, AU - Maeda-Yamamoto,M, PY - 2001/7/12/pubmed PY - 2001/8/10/medline PY - 2001/7/12/entrez SP - 816 EP - 20 JF - The Analyst JO - Analyst VL - 126 IS - 6 N2 - A high-performance liquid chromatographic method with electrochemical detection was developed for the determination of twelve tea catechins including four major catechins: epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG) and epigallocatechin gallate (EGCG); four of their epimers at the C-2 position, C, GC, CG and GCG; and four methylated catechin derivatives, epigallocatechin-3-O-(3-O-methyl)gallate, gallocatechin-3-O-(3-O-methyl)gallate, epigallocatechin-3-O-(4-O-methyl)gallate and epicatechin-3-O-(3-O-methyl)gallate. These catechins were separated on an ODS C18 reversed-phase column by isocratic elution with 0.1 M NaH2PO4 buffer (pH 2.5)-acetonitrile (87:13) containing 0.1 mM EDTA.2Na. The detection limits (S/N = 3) of these catechins were approximately 10-40 pmol ml-1 at an applied voltage of 600 mV. Extracting these catechins from tea leaf powder with H2O-acetonitrile (1:1) at 30 degrees C for 40 min inhibited the epimerization at C-2 significantly from these epicatechins compared to extraction with hot water at 90 degrees C. This analytical method is sensitive to and appropriate for the simultaneous determination of various biologically active catechins in green tea. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/11445943/Simultaneous_determination_of_twelve_tea_catechins_by_high_performance_liquid_chromatography_with_electrochemical_detection_ L2 - https://doi.org/10.1039/b102541b DB - PRIME DP - Unbound Medicine ER -