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Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant.
Infect Immun 2001; 69(8):4816-22II

Abstract

The P39 and the bacterioferrin (BFR) antigens of Brucella melitensis 16M were previously identified as T dominant antigens able to induce both delayed-type hypersensivity in sensitized guinea pigs and in vitro gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells from infected cattle. Here, we analyzed the potential for these antigens to function as a subunitary vaccine against Brucella abortus infection in BALB/c mice, and we characterized the humoral and cellular immune responses induced. Mice were injected with each of the recombinant proteins alone or adjuvanted with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. Mice immunized with the recombinant antigens with CpG ODN were the only group demonstrating both significant IFN-gamma production and T-cell proliferation in response to either Brucella extract or to the respective antigen. The same conclusion holds true for the antibody response, which was only demonstrated in mice immunized with recombinant antigens mixed with CpG ODN. The antibody titers (both immunoglobulin G1 [IgG1] and IgG2a) induced by P39 immunization were higher than the titers induced by BFR (only IgG2a). Using a B. abortus 544 challenge, the level of protection was analyzed and compared to the protection conferred by one immunization with the vaccine strain B19. Immunization with P39 and CpG ODN gave a level of protection comparable to the one conferred by B19 at 4 weeks postchallenge, and the mice were still significantly protected at 8 weeks postchallenge, although to a lesser extent than the B19-vaccinated group. Intriguingly, no protection was detected after BFR vaccination. All other groups did not demonstrate any protection.

Authors+Show Affiliations

Unité de Recherche en Biologie Moléculaire, Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11447155

Citation

Al-Mariri, A, et al. "Protection of BALB/c Mice Against Brucella Abortus 544 Challenge By Vaccination With Bacterioferritin or P39 Recombinant Proteins With CpG Oligodeoxynucleotides as Adjuvant." Infection and Immunity, vol. 69, no. 8, 2001, pp. 4816-22.
Al-Mariri A, Tibor A, Mertens P, et al. Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant. Infect Immun. 2001;69(8):4816-22.
Al-Mariri, A., Tibor, A., Mertens, P., De Bolle, X., Michel, P., Godefroid, J., ... Letesson, J. J. (2001). Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant. Infection and Immunity, 69(8), pp. 4816-22.
Al-Mariri A, et al. Protection of BALB/c Mice Against Brucella Abortus 544 Challenge By Vaccination With Bacterioferritin or P39 Recombinant Proteins With CpG Oligodeoxynucleotides as Adjuvant. Infect Immun. 2001;69(8):4816-22. PubMed PMID: 11447155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant. AU - Al-Mariri,A, AU - Tibor,A, AU - Mertens,P, AU - De Bolle,X, AU - Michel,P, AU - Godefroid,J, AU - Walravens,K, AU - Letesson,J J, PY - 2001/7/12/pubmed PY - 2001/8/24/medline PY - 2001/7/12/entrez SP - 4816 EP - 22 JF - Infection and immunity JO - Infect. Immun. VL - 69 IS - 8 N2 - The P39 and the bacterioferrin (BFR) antigens of Brucella melitensis 16M were previously identified as T dominant antigens able to induce both delayed-type hypersensivity in sensitized guinea pigs and in vitro gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells from infected cattle. Here, we analyzed the potential for these antigens to function as a subunitary vaccine against Brucella abortus infection in BALB/c mice, and we characterized the humoral and cellular immune responses induced. Mice were injected with each of the recombinant proteins alone or adjuvanted with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. Mice immunized with the recombinant antigens with CpG ODN were the only group demonstrating both significant IFN-gamma production and T-cell proliferation in response to either Brucella extract or to the respective antigen. The same conclusion holds true for the antibody response, which was only demonstrated in mice immunized with recombinant antigens mixed with CpG ODN. The antibody titers (both immunoglobulin G1 [IgG1] and IgG2a) induced by P39 immunization were higher than the titers induced by BFR (only IgG2a). Using a B. abortus 544 challenge, the level of protection was analyzed and compared to the protection conferred by one immunization with the vaccine strain B19. Immunization with P39 and CpG ODN gave a level of protection comparable to the one conferred by B19 at 4 weeks postchallenge, and the mice were still significantly protected at 8 weeks postchallenge, although to a lesser extent than the B19-vaccinated group. Intriguingly, no protection was detected after BFR vaccination. All other groups did not demonstrate any protection. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/11447155/Protection_of_BALB/c_mice_against_Brucella_abortus_544_challenge_by_vaccination_with_bacterioferritin_or_P39_recombinant_proteins_with_CpG_oligodeoxynucleotides_as_adjuvant_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=11447155 DB - PRIME DP - Unbound Medicine ER -