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Diagnosis of pancreatic adenocarcinoma by detection of human telomerase reverse transcriptase messenger RNA in pancreatic juice with sample qualification.
Clin Cancer Res. 2001 Jul; 7(7):1976-81.CC

Abstract

PURPOSE

We evaluated the diagnostic efficacy of detection of human telomerase reverse transcriptase (hTERT) message, a catalytic domain of human telomerase, in endoscopic retrograde pancreatography (ERP)-derived pancreatic juice.

EXPERIMENTAL DESIGN

Both hTERT and CD25 expression were detected by reverse transcription-PCR (RT-PCR) in 17 patients with pancreatic adenocarcinoma (PC), 12 patients with chronic pancreatitis (CP), and 7 patients with no ERP abnormality (N). In the same patients, beta-actin message was semiquantified by competitive RT-PCR. K-ras codon 12 mutations were concomitantly analyzed by enriched PCR-SSCP in 11 and 7 PC and CP cases, respectively.

RESULTS

Expression of hTERT was detected in 88% of PC cases and 17% of CP cases but not in the normal control (N). Alterations in K-ras were detected in 73% of PC cases and 57% of CP cases, respectively. beta-Actin mRNA was expressed in >3.0 x 10(1) copies/microl in all but two PC cases in which hTERT mRNA was not detected. CD25-positive and -negative peripheral lymphocytes were isolated from a normal volunteer using a fluorescent activating cell sorter. The hTERT message was detected in CD25-positive peripheral lymphocytes and in 18, 25, and 0% of the pancreatic juice samples from PC, CP, and N cases, respectively. All CP cases expressing hTERT message were also CD25 positive.

CONCLUSIONS

These results suggest that detection of hTERT mRNA in pancreatic juice is a powerful tool to discriminate PC from CP, particularly when the samples are qualified against beta-actin mRNA levels and contaminating CD25-positive lymphocytes.

Authors+Show Affiliations

Department of Molecular Genetics, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8122, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11448913

Citation

Seki, K, et al. "Diagnosis of Pancreatic Adenocarcinoma By Detection of Human Telomerase Reverse Transcriptase Messenger RNA in Pancreatic Juice With Sample Qualification." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 7, no. 7, 2001, pp. 1976-81.
Seki K, Suda T, Aoyagi Y, et al. Diagnosis of pancreatic adenocarcinoma by detection of human telomerase reverse transcriptase messenger RNA in pancreatic juice with sample qualification. Clin Cancer Res. 2001;7(7):1976-81.
Seki, K., Suda, T., Aoyagi, Y., Sugawara, S., Natsui, M., Motoyama, H., Shirai, Y., Sekine, T., Kawai, H., Mita, Y., Waguri, N., Kuroiwa, T., Igarashi, M., & Asakura, H. (2001). Diagnosis of pancreatic adenocarcinoma by detection of human telomerase reverse transcriptase messenger RNA in pancreatic juice with sample qualification. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 7(7), 1976-81.
Seki K, et al. Diagnosis of Pancreatic Adenocarcinoma By Detection of Human Telomerase Reverse Transcriptase Messenger RNA in Pancreatic Juice With Sample Qualification. Clin Cancer Res. 2001;7(7):1976-81. PubMed PMID: 11448913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of pancreatic adenocarcinoma by detection of human telomerase reverse transcriptase messenger RNA in pancreatic juice with sample qualification. AU - Seki,K, AU - Suda,T, AU - Aoyagi,Y, AU - Sugawara,S, AU - Natsui,M, AU - Motoyama,H, AU - Shirai,Y, AU - Sekine,T, AU - Kawai,H, AU - Mita,Y, AU - Waguri,N, AU - Kuroiwa,T, AU - Igarashi,M, AU - Asakura,H, PY - 2001/7/13/pubmed PY - 2001/10/26/medline PY - 2001/7/13/entrez SP - 1976 EP - 81 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin. Cancer Res. VL - 7 IS - 7 N2 - PURPOSE: We evaluated the diagnostic efficacy of detection of human telomerase reverse transcriptase (hTERT) message, a catalytic domain of human telomerase, in endoscopic retrograde pancreatography (ERP)-derived pancreatic juice. EXPERIMENTAL DESIGN: Both hTERT and CD25 expression were detected by reverse transcription-PCR (RT-PCR) in 17 patients with pancreatic adenocarcinoma (PC), 12 patients with chronic pancreatitis (CP), and 7 patients with no ERP abnormality (N). In the same patients, beta-actin message was semiquantified by competitive RT-PCR. K-ras codon 12 mutations were concomitantly analyzed by enriched PCR-SSCP in 11 and 7 PC and CP cases, respectively. RESULTS: Expression of hTERT was detected in 88% of PC cases and 17% of CP cases but not in the normal control (N). Alterations in K-ras were detected in 73% of PC cases and 57% of CP cases, respectively. beta-Actin mRNA was expressed in >3.0 x 10(1) copies/microl in all but two PC cases in which hTERT mRNA was not detected. CD25-positive and -negative peripheral lymphocytes were isolated from a normal volunteer using a fluorescent activating cell sorter. The hTERT message was detected in CD25-positive peripheral lymphocytes and in 18, 25, and 0% of the pancreatic juice samples from PC, CP, and N cases, respectively. All CP cases expressing hTERT message were also CD25 positive. CONCLUSIONS: These results suggest that detection of hTERT mRNA in pancreatic juice is a powerful tool to discriminate PC from CP, particularly when the samples are qualified against beta-actin mRNA levels and contaminating CD25-positive lymphocytes. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/11448913/Diagnosis_of_pancreatic_adenocarcinoma_by_detection_of_human_telomerase_reverse_transcriptase_messenger_RNA_in_pancreatic_juice_with_sample_qualification_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11448913 DB - PRIME DP - Unbound Medicine ER -