TY - JOUR T1 - Diagnosis of pancreatic adenocarcinoma by detection of human telomerase reverse transcriptase messenger RNA in pancreatic juice with sample qualification. AU - Seki,K, AU - Suda,T, AU - Aoyagi,Y, AU - Sugawara,S, AU - Natsui,M, AU - Motoyama,H, AU - Shirai,Y, AU - Sekine,T, AU - Kawai,H, AU - Mita,Y, AU - Waguri,N, AU - Kuroiwa,T, AU - Igarashi,M, AU - Asakura,H, PY - 2001/7/13/pubmed PY - 2001/10/26/medline PY - 2001/7/13/entrez SP - 1976 EP - 81 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin Cancer Res VL - 7 IS - 7 N2 - PURPOSE: We evaluated the diagnostic efficacy of detection of human telomerase reverse transcriptase (hTERT) message, a catalytic domain of human telomerase, in endoscopic retrograde pancreatography (ERP)-derived pancreatic juice. EXPERIMENTAL DESIGN: Both hTERT and CD25 expression were detected by reverse transcription-PCR (RT-PCR) in 17 patients with pancreatic adenocarcinoma (PC), 12 patients with chronic pancreatitis (CP), and 7 patients with no ERP abnormality (N). In the same patients, beta-actin message was semiquantified by competitive RT-PCR. K-ras codon 12 mutations were concomitantly analyzed by enriched PCR-SSCP in 11 and 7 PC and CP cases, respectively. RESULTS: Expression of hTERT was detected in 88% of PC cases and 17% of CP cases but not in the normal control (N). Alterations in K-ras were detected in 73% of PC cases and 57% of CP cases, respectively. beta-Actin mRNA was expressed in >3.0 x 10(1) copies/microl in all but two PC cases in which hTERT mRNA was not detected. CD25-positive and -negative peripheral lymphocytes were isolated from a normal volunteer using a fluorescent activating cell sorter. The hTERT message was detected in CD25-positive peripheral lymphocytes and in 18, 25, and 0% of the pancreatic juice samples from PC, CP, and N cases, respectively. All CP cases expressing hTERT message were also CD25 positive. CONCLUSIONS: These results suggest that detection of hTERT mRNA in pancreatic juice is a powerful tool to discriminate PC from CP, particularly when the samples are qualified against beta-actin mRNA levels and contaminating CD25-positive lymphocytes. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/11448913/Diagnosis_of_pancreatic_adenocarcinoma_by_detection_of_human_telomerase_reverse_transcriptase_messenger_RNA_in_pancreatic_juice_with_sample_qualification_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11448913 DB - PRIME DP - Unbound Medicine ER -