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Polyamine and redox modulation of [3H]MK-801 binding to N-methyl-D-aspartate receptors in the spinal cord and cerebral cortex.
Pharmacol Toxicol. 2001 Jun; 88(6):342-8.PT

Abstract

The pharmacology of N-methyl-D-aspartate (NMDA) receptors shows regional differences in affinity for various agonists and antagonists. We have investigated the modulatory mechanisms acting via the polyamine, redox and proton sites in the cerebral cortex and the spinal cord of adult, male rats using [3H]MK-801 binding. The affinity for glycine-independent spermine stimulation was one magnitude higher in cerebrocortical than in spinal cord membranes while the affinity for the spermine antagonist arcaine was similar. Spermine abolished the inhibiting effect of low pH in both regions. Thus, the difference in the polyamine site between the two regions seems to be restricted to agonist binding. The proportion of high affinity/total ifenprodil binding was approximately 35% both in the spinal cord and the cerebral cortex, suggesting similar relative amounts of the NMDA receptor subunit 2B. The affinity of ifenprodil to the high affinity site was however significantly higher in the cerebral cortex. Redox modulatory agents had similar effects in the two regions but spermine fully counteracted the inhibiting effect of 0.2 mM 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) in the cerebral cortex while there was only a partial effect in the spinal cord. These data show that the regional pharmacological heterogeneity involves several of the mechanisms regulating the function of the NMDA receptor. The data also indicate that the NMDA receptor subunit 2B is much more common in spinal cord than previously suggested.

Authors+Show Affiliations

Division of Geriatric Medicine, NEUROTEC, Karolinska Institutet, Stockholm, Sweden. erik.sundstrom@neurotec.ki.seNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11453375

Citation

Sundström, E, and L L. Mo. "Polyamine and Redox Modulation of [3H]MK-801 Binding to N-methyl-D-aspartate Receptors in the Spinal Cord and Cerebral Cortex." Pharmacology & Toxicology, vol. 88, no. 6, 2001, pp. 342-8.
Sundström E, Mo LL. Polyamine and redox modulation of [3H]MK-801 binding to N-methyl-D-aspartate receptors in the spinal cord and cerebral cortex. Pharmacol Toxicol. 2001;88(6):342-8.
Sundström, E., & Mo, L. L. (2001). Polyamine and redox modulation of [3H]MK-801 binding to N-methyl-D-aspartate receptors in the spinal cord and cerebral cortex. Pharmacology & Toxicology, 88(6), 342-8.
Sundström E, Mo LL. Polyamine and Redox Modulation of [3H]MK-801 Binding to N-methyl-D-aspartate Receptors in the Spinal Cord and Cerebral Cortex. Pharmacol Toxicol. 2001;88(6):342-8. PubMed PMID: 11453375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyamine and redox modulation of [3H]MK-801 binding to N-methyl-D-aspartate receptors in the spinal cord and cerebral cortex. AU - Sundström,E, AU - Mo,L L, PY - 2001/7/17/pubmed PY - 2002/7/18/medline PY - 2001/7/17/entrez SP - 342 EP - 8 JF - Pharmacology & toxicology JO - Pharmacol Toxicol VL - 88 IS - 6 N2 - The pharmacology of N-methyl-D-aspartate (NMDA) receptors shows regional differences in affinity for various agonists and antagonists. We have investigated the modulatory mechanisms acting via the polyamine, redox and proton sites in the cerebral cortex and the spinal cord of adult, male rats using [3H]MK-801 binding. The affinity for glycine-independent spermine stimulation was one magnitude higher in cerebrocortical than in spinal cord membranes while the affinity for the spermine antagonist arcaine was similar. Spermine abolished the inhibiting effect of low pH in both regions. Thus, the difference in the polyamine site between the two regions seems to be restricted to agonist binding. The proportion of high affinity/total ifenprodil binding was approximately 35% both in the spinal cord and the cerebral cortex, suggesting similar relative amounts of the NMDA receptor subunit 2B. The affinity of ifenprodil to the high affinity site was however significantly higher in the cerebral cortex. Redox modulatory agents had similar effects in the two regions but spermine fully counteracted the inhibiting effect of 0.2 mM 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) in the cerebral cortex while there was only a partial effect in the spinal cord. These data show that the regional pharmacological heterogeneity involves several of the mechanisms regulating the function of the NMDA receptor. The data also indicate that the NMDA receptor subunit 2B is much more common in spinal cord than previously suggested. SN - 0901-9928 UR - https://www.unboundmedicine.com/medline/citation/11453375/Polyamine_and_redox_modulation_of_[3H]MK_801_binding_to_N_methyl_D_aspartate_receptors_in_the_spinal_cord_and_cerebral_cortex_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11453375.ui DB - PRIME DP - Unbound Medicine ER -