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The fatty acid composition of chylomicron remnants influences their binding and internalization by isolated hepatocytes.
Eur J Biochem. 2001 Jul; 268(14):3983-92.EJ

Abstract

The binding and internalization of (125)I-labelled chylomicron remnants derived from palm, olive, corn, or fish oil (rich in saturated, monounsaturated, n-6, or n-3 polyunsaturated fatty acids, respectively) by hepatocytes from rats fed a low-fat diet or a diet supplemented with the corresponding fat for 21 days was investigated. In hepatocytes from rats fed the low-fat diet, the association of radioactivity with the cells at 4 degrees C (a measure of initial binding only) was similar with all types of remnants tested, but was more rapid at 37 degrees C (a measure of binding plus internalization) when fish oil, as compared to olive, corn or palm oil remnants, was used, and similar differences in the internalization of the particles were observed. In contrast, when hepatocytes from rats fed the fat-supplemented diets were used, the rate of association at 37 degrees C of remnants with cells from rats fed palm, corn or fish oil was similar, and higher than that found with cells from animals fed olive oil, and in this case these differences were mainly due to changes in the binding of the particles to the cells at 4 degrees C. Both excess low-density lipoprotein (LDL), which inhibits remnant uptake by the LDL receptor, and lactoferrin, which blocks the LDL receptor-related protein (LRP), were found to decrease the association of the remnants with cells from rats fed the low-fat and high-fat diets. However, in hepatocytes from animals given the low-fat diet, most of the differences between the various types of particle were retained in the presence of lactoferrin, but abolished in the presence of LDL. In contrast, in cells from rats fed the high-fat diets, the differences were reduced by both lactoferrin and LDL. These findings demonstrate that the hepatic uptake of chylomicron remnants is influenced both by the fatty acid composition of the particles, and by longer-term adaptive changes in liver tissue, and suggest that the former effects are mediated mainly by the LDL receptor, while the latter may involve both the LDL receptor and the LRP.

Authors+Show Affiliations

Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11453992

Citation

Lambert, M S., et al. "The Fatty Acid Composition of Chylomicron Remnants Influences Their Binding and Internalization By Isolated Hepatocytes." European Journal of Biochemistry, vol. 268, no. 14, 2001, pp. 3983-92.
Lambert MS, Avella MA, Berhane Y, et al. The fatty acid composition of chylomicron remnants influences their binding and internalization by isolated hepatocytes. Eur J Biochem. 2001;268(14):3983-92.
Lambert, M. S., Avella, M. A., Berhane, Y., Shervill, E., & Botham, K. M. (2001). The fatty acid composition of chylomicron remnants influences their binding and internalization by isolated hepatocytes. European Journal of Biochemistry, 268(14), 3983-92.
Lambert MS, et al. The Fatty Acid Composition of Chylomicron Remnants Influences Their Binding and Internalization By Isolated Hepatocytes. Eur J Biochem. 2001;268(14):3983-92. PubMed PMID: 11453992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fatty acid composition of chylomicron remnants influences their binding and internalization by isolated hepatocytes. AU - Lambert,M S, AU - Avella,M A, AU - Berhane,Y, AU - Shervill,E, AU - Botham,K M, PY - 2001/7/17/pubmed PY - 2001/8/17/medline PY - 2001/7/17/entrez SP - 3983 EP - 92 JF - European journal of biochemistry JO - Eur. J. Biochem. VL - 268 IS - 14 N2 - The binding and internalization of (125)I-labelled chylomicron remnants derived from palm, olive, corn, or fish oil (rich in saturated, monounsaturated, n-6, or n-3 polyunsaturated fatty acids, respectively) by hepatocytes from rats fed a low-fat diet or a diet supplemented with the corresponding fat for 21 days was investigated. In hepatocytes from rats fed the low-fat diet, the association of radioactivity with the cells at 4 degrees C (a measure of initial binding only) was similar with all types of remnants tested, but was more rapid at 37 degrees C (a measure of binding plus internalization) when fish oil, as compared to olive, corn or palm oil remnants, was used, and similar differences in the internalization of the particles were observed. In contrast, when hepatocytes from rats fed the fat-supplemented diets were used, the rate of association at 37 degrees C of remnants with cells from rats fed palm, corn or fish oil was similar, and higher than that found with cells from animals fed olive oil, and in this case these differences were mainly due to changes in the binding of the particles to the cells at 4 degrees C. Both excess low-density lipoprotein (LDL), which inhibits remnant uptake by the LDL receptor, and lactoferrin, which blocks the LDL receptor-related protein (LRP), were found to decrease the association of the remnants with cells from rats fed the low-fat and high-fat diets. However, in hepatocytes from animals given the low-fat diet, most of the differences between the various types of particle were retained in the presence of lactoferrin, but abolished in the presence of LDL. In contrast, in cells from rats fed the high-fat diets, the differences were reduced by both lactoferrin and LDL. These findings demonstrate that the hepatic uptake of chylomicron remnants is influenced both by the fatty acid composition of the particles, and by longer-term adaptive changes in liver tissue, and suggest that the former effects are mediated mainly by the LDL receptor, while the latter may involve both the LDL receptor and the LRP. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/11453992/The_fatty_acid_composition_of_chylomicron_remnants_influences_their_binding_and_internalization_by_isolated_hepatocytes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=2001&volume=268&issue=14&spage=3983 DB - PRIME DP - Unbound Medicine ER -