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Extracellular polysaccharides of modified strains of Erwinia spp.
Carbohydr Res. 2001 Jul 19; 333(4):295-302.CR

Abstract

The structure of the extracellular polysaccharide (EPS) produced by Erwinia chrysanthemi strain A2148 has been determined using low pressure size-exclusion and anion-exchange chromatographies, high pH anion-exchange chromatography, glycosyl-linkage analysis, and 1D 1H NMR spectroscopy. The polysaccharide is structurally similar, if not identical, to the EPS produced by E. chrysanthemi strain A350. A streptomycin-resistant strain of E. chrysanthemi Ech6 (Ech6S(+)) has been generated and has an elevated production of EPS, as does a streptomycin-resistant strain (Ech9Sm6) of E. chrysanthemi Ech9. These modified E. chrysanthemi spp. have been ribotyped and found to be closely related to their parent strains.

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Authors+Show Affiliations

Yang BY
Department of Biochemistry, College of Medicine, University of Iowa, 52242, Iowa City, IA, USA.
Brand JM
No affiliation info available
Gray JS
No affiliation info available
Montgomery R
No affiliation info available

MeSH

Carbohydrate SequenceChromatographyDickeya chrysanthemiDrug Resistance, MicrobialMolecular Sequence DataMolecular StructureNuclear Magnetic Resonance, BiomolecularPolysaccharides, BacterialRibotypingStreptomycin

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11454336

Citation

Yang, B Y., et al. "Extracellular Polysaccharides of Modified Strains of Erwinia Spp." Carbohydrate Research, vol. 333, no. 4, 2001, pp. 295-302.
Yang BY, Brand JM, Gray JS, et al. Extracellular polysaccharides of modified strains of Erwinia spp. Carbohydr Res. 2001;333(4):295-302.
Yang, B. Y., Brand, J. M., Gray, J. S., & Montgomery, R. (2001). Extracellular polysaccharides of modified strains of Erwinia spp. Carbohydrate Research, 333(4), 295-302.
Yang BY, et al. Extracellular Polysaccharides of Modified Strains of Erwinia Spp. Carbohydr Res. 2001 Jul 19;333(4):295-302. PubMed PMID: 11454336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extracellular polysaccharides of modified strains of Erwinia spp. AU - Yang,B Y, AU - Brand,J M, AU - Gray,J S, AU - Montgomery,R, PY - 2001/7/17/pubmed PY - 2001/11/3/medline PY - 2001/7/17/entrez SP - 295 EP - 302 JF - Carbohydrate research JO - Carbohydr Res VL - 333 IS - 4 N2 - The structure of the extracellular polysaccharide (EPS) produced by Erwinia chrysanthemi strain A2148 has been determined using low pressure size-exclusion and anion-exchange chromatographies, high pH anion-exchange chromatography, glycosyl-linkage analysis, and 1D 1H NMR spectroscopy. The polysaccharide is structurally similar, if not identical, to the EPS produced by E. chrysanthemi strain A350. A streptomycin-resistant strain of E. chrysanthemi Ech6 (Ech6S(+)) has been generated and has an elevated production of EPS, as does a streptomycin-resistant strain (Ech9Sm6) of E. chrysanthemi Ech9. These modified E. chrysanthemi spp. have been ribotyped and found to be closely related to their parent strains. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/11454336/Extracellular_polysaccharides_of_modified_strains_of_Erwinia_spp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008621501001550 DB - PRIME DP - Unbound Medicine ER -